Effect of solid to water ratio, time and temperature on aqueous extraction of gallic acid from labisia pumila var alata of Malaysia

Labisa pumila var alata contains bioactive compounds such as gallic acid that is of use for pharmaceutical and nutraceutical industries. Aqueous extraction is the traditional method that extracts phytochemicals from plant material. The study aimed to find the optimum condition for maximum gallic aci...

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Main Authors: Noor Adilah, Md Salehan, Nailaa, Aishath, Azilah, Ajit, Ahmad Ziad, Sulaiman
Format: Article
Language:English
Published: PCSIR Scientific Information Centre 2020
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/31088/
http://umpir.ump.edu.my/id/eprint/31088/1/Effect%20of%20solid%20to%20water%20ratio%20time%20and%20temperature.pdf
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author Noor Adilah, Md Salehan
Nailaa, Aishath
Azilah, Ajit
Ahmad Ziad, Sulaiman
author_facet Noor Adilah, Md Salehan
Nailaa, Aishath
Azilah, Ajit
Ahmad Ziad, Sulaiman
author_sort Noor Adilah, Md Salehan
building UMP Institutional Repository
collection Online Access
description Labisa pumila var alata contains bioactive compounds such as gallic acid that is of use for pharmaceutical and nutraceutical industries. Aqueous extraction is the traditional method that extracts phytochemicals from plant material. The study aimed to find the optimum condition for maximum gallic acid yield from ground L. pumila leaves using aqueous extraction. The results revealed that the maxium gallic acid yield obtained was at 1:10 sample to water ratio for 8 h at 50 °C. The maximum yield of gallic acid obtained was 1.025 mg gallic acid per g dried leaves (mg/g). The identification of gallic acid was done on Liquid Chromatograph Mass Spectrometer Quadrupole Time-of-Flight (LCMS-Q-TOF) by comparison to that of reference standard. The morphological structure of the extract that was obtained at optimum condition showed less denaturation of cell wall which indicates that still some gallic acid could be trapped in the sample matrix and other methods need to be employed to release them.
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institution Universiti Malaysia Pahang
institution_category Local University
language English
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publishDate 2020
publisher PCSIR Scientific Information Centre
recordtype eprints
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spelling ump-310882021-04-28T04:57:39Z http://umpir.ump.edu.my/id/eprint/31088/ Effect of solid to water ratio, time and temperature on aqueous extraction of gallic acid from labisia pumila var alata of Malaysia Noor Adilah, Md Salehan Nailaa, Aishath Azilah, Ajit Ahmad Ziad, Sulaiman TP Chemical technology Labisa pumila var alata contains bioactive compounds such as gallic acid that is of use for pharmaceutical and nutraceutical industries. Aqueous extraction is the traditional method that extracts phytochemicals from plant material. The study aimed to find the optimum condition for maximum gallic acid yield from ground L. pumila leaves using aqueous extraction. The results revealed that the maxium gallic acid yield obtained was at 1:10 sample to water ratio for 8 h at 50 °C. The maximum yield of gallic acid obtained was 1.025 mg gallic acid per g dried leaves (mg/g). The identification of gallic acid was done on Liquid Chromatograph Mass Spectrometer Quadrupole Time-of-Flight (LCMS-Q-TOF) by comparison to that of reference standard. The morphological structure of the extract that was obtained at optimum condition showed less denaturation of cell wall which indicates that still some gallic acid could be trapped in the sample matrix and other methods need to be employed to release them. PCSIR Scientific Information Centre 2020-07-15 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/31088/1/Effect%20of%20solid%20to%20water%20ratio%20time%20and%20temperature.pdf Noor Adilah, Md Salehan and Nailaa, Aishath and Azilah, Ajit and Ahmad Ziad, Sulaiman (2020) Effect of solid to water ratio, time and temperature on aqueous extraction of gallic acid from labisia pumila var alata of Malaysia. Pakistan Journal of Scientific and Industrial Research, Series B: Biological Sciences, 63 (2). pp. 93-99. ISSN 2223-2567. (Published) https://v2.pjsir.org/index.php/biological-sciences/article/view/1273
spellingShingle TP Chemical technology
Noor Adilah, Md Salehan
Nailaa, Aishath
Azilah, Ajit
Ahmad Ziad, Sulaiman
Effect of solid to water ratio, time and temperature on aqueous extraction of gallic acid from labisia pumila var alata of Malaysia
title Effect of solid to water ratio, time and temperature on aqueous extraction of gallic acid from labisia pumila var alata of Malaysia
title_full Effect of solid to water ratio, time and temperature on aqueous extraction of gallic acid from labisia pumila var alata of Malaysia
title_fullStr Effect of solid to water ratio, time and temperature on aqueous extraction of gallic acid from labisia pumila var alata of Malaysia
title_full_unstemmed Effect of solid to water ratio, time and temperature on aqueous extraction of gallic acid from labisia pumila var alata of Malaysia
title_short Effect of solid to water ratio, time and temperature on aqueous extraction of gallic acid from labisia pumila var alata of Malaysia
title_sort effect of solid to water ratio, time and temperature on aqueous extraction of gallic acid from labisia pumila var alata of malaysia
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/31088/
http://umpir.ump.edu.my/id/eprint/31088/
http://umpir.ump.edu.my/id/eprint/31088/1/Effect%20of%20solid%20to%20water%20ratio%20time%20and%20temperature.pdf