Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA)
Twelve probiotic Lactobacillus strains for poultry were characterised by amplified ribosomal DNA restriction analysis (ARDRA) using Sau3AI, TaqI, HaeIII and AluI restriction endonucleases. Species-specific and strain-specific restriction patterns were observed from the bacterial strains. Numerical a...
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| Format: | Conference or Workshop Item |
| Language: | English English |
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Trans Tech Publications, Switzerland
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/30474/ http://umpir.ump.edu.my/id/eprint/30474/1/5.1%20Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified.pdf http://umpir.ump.edu.my/id/eprint/30474/7/Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified%20ribosomal%20dna%20restriction%20analysis.pdf |
| _version_ | 1848823522680373248 |
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| author | Lee, Chin Mei Sieo, Chin Chin Norhani, Abdullah Ho, Yin Wan |
| author_facet | Lee, Chin Mei Sieo, Chin Chin Norhani, Abdullah Ho, Yin Wan |
| author_sort | Lee, Chin Mei |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Twelve probiotic Lactobacillus strains for poultry were characterised by amplified ribosomal DNA restriction analysis (ARDRA) using Sau3AI, TaqI, HaeIII and AluI restriction endonucleases. Species-specific and strain-specific restriction patterns were observed from the bacterial strains. Numerical analysis of composite analysis of ARDRA exhibited D value of 0.8456. Whereas, the caculated D values of ARDRA patterns generated by Sau3AI, TaqI, HaeIII and AluI were 0.8309, 0.8382,0.8088 and 0.8088, repectively. Composite analysis of ARDRA was the most discriminative method when compared to the individual analysis. ARDRA could distinguished L. reuteri C 10 and L. panis C 17 into single strains. The 16S rRNA gene restriction patterns were also able to group L. gallinarum I 16 and I 26 into single strains. Lactobacillus brevis I 12, I 23, I 25, I 211 and I 218 seem to be multiple clones of the same bacterial strain as are L. reuteri C 1 and C 16. ARDRA is a valuable fingerprinting method to discriminate probiotic Lactobacillus strains. |
| first_indexed | 2025-11-15T02:58:29Z |
| format | Conference or Workshop Item |
| id | ump-30474 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T02:58:29Z |
| publishDate | 2020 |
| publisher | Trans Tech Publications, Switzerland |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-304742021-02-26T03:25:54Z http://umpir.ump.edu.my/id/eprint/30474/ Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA) Lee, Chin Mei Sieo, Chin Chin Norhani, Abdullah Ho, Yin Wan TA Engineering (General). Civil engineering (General) Twelve probiotic Lactobacillus strains for poultry were characterised by amplified ribosomal DNA restriction analysis (ARDRA) using Sau3AI, TaqI, HaeIII and AluI restriction endonucleases. Species-specific and strain-specific restriction patterns were observed from the bacterial strains. Numerical analysis of composite analysis of ARDRA exhibited D value of 0.8456. Whereas, the caculated D values of ARDRA patterns generated by Sau3AI, TaqI, HaeIII and AluI were 0.8309, 0.8382,0.8088 and 0.8088, repectively. Composite analysis of ARDRA was the most discriminative method when compared to the individual analysis. ARDRA could distinguished L. reuteri C 10 and L. panis C 17 into single strains. The 16S rRNA gene restriction patterns were also able to group L. gallinarum I 16 and I 26 into single strains. Lactobacillus brevis I 12, I 23, I 25, I 211 and I 218 seem to be multiple clones of the same bacterial strain as are L. reuteri C 1 and C 16. ARDRA is a valuable fingerprinting method to discriminate probiotic Lactobacillus strains. Trans Tech Publications, Switzerland 2020 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/30474/1/5.1%20Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified.pdf pdf en http://umpir.ump.edu.my/id/eprint/30474/7/Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified%20ribosomal%20dna%20restriction%20analysis.pdf Lee, Chin Mei and Sieo, Chin Chin and Norhani, Abdullah and Ho, Yin Wan (2020) Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA). In: Symposium on Industrial Science and Technology, SISTEC 2019 , 22-23 Aug. 2019 , Kuantan, Malaysia. pp. 302-308., 981. ISSN 1662-9752 (Published) https://doi.org/10.4028/www.scientific.net/MSF.981.302 |
| spellingShingle | TA Engineering (General). Civil engineering (General) Lee, Chin Mei Sieo, Chin Chin Norhani, Abdullah Ho, Yin Wan Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA) |
| title | Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA) |
| title_full | Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA) |
| title_fullStr | Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA) |
| title_full_unstemmed | Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA) |
| title_short | Characterisations of probiotic Lactobacillus strains by amplified ribosomal dna restriction analysis (ARDRA) |
| title_sort | characterisations of probiotic lactobacillus strains by amplified ribosomal dna restriction analysis (ardra) |
| topic | TA Engineering (General). Civil engineering (General) |
| url | http://umpir.ump.edu.my/id/eprint/30474/ http://umpir.ump.edu.my/id/eprint/30474/ http://umpir.ump.edu.my/id/eprint/30474/1/5.1%20Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified.pdf http://umpir.ump.edu.my/id/eprint/30474/7/Characterisations%20of%20probiotic%20Lactobacillus%20strains%20by%20amplified%20ribosomal%20dna%20restriction%20analysis.pdf |