Optimization of Rebaudioside A extraction from stevia leaves using response surface methodology

Rebaudioside A or Reb A is an extractable component from stevia leaves or scientifically known as Stevia rebaudiana. Reb A is famous for its exceptional sweetness and widely used as a non-caloric sweetener. Its potential widespread use requires an easy and effective extraction method. In this study,...

Full description

Bibliographic Details
Main Authors: Kalaiarasan, Muthusamy, Mimi Sakinah, A. M.
Format: Conference or Workshop Item
Language:English
Published: IOP Publishing 2020
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/30010/
http://umpir.ump.edu.my/id/eprint/30010/7/Optimization%20of%20Rebaudioside%20A%20extraction%20from%20stevia.pdf
_version_ 1848823413390442496
author Kalaiarasan, Muthusamy
Mimi Sakinah, A. M.
author_facet Kalaiarasan, Muthusamy
Mimi Sakinah, A. M.
author_sort Kalaiarasan, Muthusamy
building UMP Institutional Repository
collection Online Access
description Rebaudioside A or Reb A is an extractable component from stevia leaves or scientifically known as Stevia rebaudiana. Reb A is famous for its exceptional sweetness and widely used as a non-caloric sweetener. Its potential widespread use requires an easy and effective extraction method. In this study, extraction of Reb A from stevia leaves with parameters such as temperature, material ratio and extraction time were investigated. Time of extraction were 1, 1.5, 2, 2.5 and 3 hours. Temperature was varied at 400C, 450C, 500C, 550C and 600C. Material ratio was 1:5, 1:10, 1:15, 1:20 and 1:25. Then, using Design Expert software, the ranges of the parameters were entered in the Central Composite Design (CCD) to create 20 different combinations of parameters for extraction. After executing the experiments, the yield of Reb A obtained was keyed in Response Surface Methodology (RSM) for optimization. Finally, the optimum condition was tested and validated by calculating the percentage error. Since the percentage error was less than 10%, the optimum condition, which is, 350C, 1:6 material ratio and 5 hours of extraction was accepted
first_indexed 2025-11-15T02:56:44Z
format Conference or Workshop Item
id ump-30010
institution Universiti Malaysia Pahang
institution_category Local University
language English
last_indexed 2025-11-15T02:56:44Z
publishDate 2020
publisher IOP Publishing
recordtype eprints
repository_type Digital Repository
spelling ump-300102021-09-06T02:39:41Z http://umpir.ump.edu.my/id/eprint/30010/ Optimization of Rebaudioside A extraction from stevia leaves using response surface methodology Kalaiarasan, Muthusamy Mimi Sakinah, A. M. T Technology (General) Rebaudioside A or Reb A is an extractable component from stevia leaves or scientifically known as Stevia rebaudiana. Reb A is famous for its exceptional sweetness and widely used as a non-caloric sweetener. Its potential widespread use requires an easy and effective extraction method. In this study, extraction of Reb A from stevia leaves with parameters such as temperature, material ratio and extraction time were investigated. Time of extraction were 1, 1.5, 2, 2.5 and 3 hours. Temperature was varied at 400C, 450C, 500C, 550C and 600C. Material ratio was 1:5, 1:10, 1:15, 1:20 and 1:25. Then, using Design Expert software, the ranges of the parameters were entered in the Central Composite Design (CCD) to create 20 different combinations of parameters for extraction. After executing the experiments, the yield of Reb A obtained was keyed in Response Surface Methodology (RSM) for optimization. Finally, the optimum condition was tested and validated by calculating the percentage error. Since the percentage error was less than 10%, the optimum condition, which is, 350C, 1:6 material ratio and 5 hours of extraction was accepted IOP Publishing 2020 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/30010/7/Optimization%20of%20Rebaudioside%20A%20extraction%20from%20stevia.pdf Kalaiarasan, Muthusamy and Mimi Sakinah, A. M. (2020) Optimization of Rebaudioside A extraction from stevia leaves using response surface methodology. In: IOP Conference Series: Materials Science and Engineering, Energy Security and Chemical Engineering Congress , 17-19 July 2019 , Kuala Lumpur, Malaysia. pp. 1-10., 736 (062024). ISSN 1757-899X (Published) https://doi.org/10.1088/1757-899X/736/6/062024
spellingShingle T Technology (General)
Kalaiarasan, Muthusamy
Mimi Sakinah, A. M.
Optimization of Rebaudioside A extraction from stevia leaves using response surface methodology
title Optimization of Rebaudioside A extraction from stevia leaves using response surface methodology
title_full Optimization of Rebaudioside A extraction from stevia leaves using response surface methodology
title_fullStr Optimization of Rebaudioside A extraction from stevia leaves using response surface methodology
title_full_unstemmed Optimization of Rebaudioside A extraction from stevia leaves using response surface methodology
title_short Optimization of Rebaudioside A extraction from stevia leaves using response surface methodology
title_sort optimization of rebaudioside a extraction from stevia leaves using response surface methodology
topic T Technology (General)
url http://umpir.ump.edu.my/id/eprint/30010/
http://umpir.ump.edu.my/id/eprint/30010/
http://umpir.ump.edu.my/id/eprint/30010/7/Optimization%20of%20Rebaudioside%20A%20extraction%20from%20stevia.pdf