A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs
Polyphenols are a class of bioactive plant secondary metabolites that are thought to have beneficial effects on gut health, such as modulation of mucosal immune and inflammatory responses and regulation of parasite burdens. Here, we examined the interactions between a polyphenol-rich diet supplement...
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| Format: | Article |
| Language: | English |
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Public Library of Science
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/29966/ http://umpir.ump.edu.my/id/eprint/29966/7/A%20polyphenol-enriched%20diet%20and%20Ascaris.pdf |
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| author | Williams, Andrew R. Krych, Lukasz Hajar Fauzan, Ahmad Nejsum, Peter Skovgaard, Kerstin Nielsen, Dennis S. Thamsborg, Stig M. |
| author_facet | Williams, Andrew R. Krych, Lukasz Hajar Fauzan, Ahmad Nejsum, Peter Skovgaard, Kerstin Nielsen, Dennis S. Thamsborg, Stig M. |
| author_sort | Williams, Andrew R. |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Polyphenols are a class of bioactive plant secondary metabolites that are thought to have beneficial effects on gut health, such as modulation of mucosal immune and inflammatory responses and regulation of parasite burdens. Here, we examined the interactions between a polyphenol-rich diet supplement and infection with the enteric nematode Ascaris suum in pigs. Pigs were fed either a basal diet or the same diet supplemented with grape pomace (GP), an industrial by-product rich in polyphenols such as oligomeric proanthocyanidins. Half of the animals in each group were then inoculated with A. suum for 14 days to assess parasite establishment, acquisition of local and systemic immune responses and effects on the gut microbiome. Despite in vitro anthelmintic activity of GP-extracts, numbers of parasite larvae in the intestine were not altered by GP-supplementation. However, the bioactive diet significantly increased numbers of eosinophils induced by A. suum infection in the duodenum, jejunum and ileum, and modulated gene expression in the jejunal mucosa of infected pigs. Both GP-supplementation and A. suum infection induced significant and apparently similar changes in the composition of the prokaryotic gut microbiota, and both also decreased concentrations of isobutyric and isovaleric acid (branched-chain short chain fatty acids) in the colon. Our results demonstrate that while a polyphenol-enriched diet in pigs may not directly influence A. suum establishment, it significantly modulates the subsequent host response to helminth infection. Our results suggest an influence of diet on immune function which may potentially be exploited to enhance immunity to helminths. |
| first_indexed | 2025-11-15T02:56:35Z |
| format | Article |
| id | ump-29966 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:56:35Z |
| publishDate | 2017 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-299662020-11-23T07:25:14Z http://umpir.ump.edu.my/id/eprint/29966/ A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs Williams, Andrew R. Krych, Lukasz Hajar Fauzan, Ahmad Nejsum, Peter Skovgaard, Kerstin Nielsen, Dennis S. Thamsborg, Stig M. QL Zoology QR Microbiology QR180 Immunology Polyphenols are a class of bioactive plant secondary metabolites that are thought to have beneficial effects on gut health, such as modulation of mucosal immune and inflammatory responses and regulation of parasite burdens. Here, we examined the interactions between a polyphenol-rich diet supplement and infection with the enteric nematode Ascaris suum in pigs. Pigs were fed either a basal diet or the same diet supplemented with grape pomace (GP), an industrial by-product rich in polyphenols such as oligomeric proanthocyanidins. Half of the animals in each group were then inoculated with A. suum for 14 days to assess parasite establishment, acquisition of local and systemic immune responses and effects on the gut microbiome. Despite in vitro anthelmintic activity of GP-extracts, numbers of parasite larvae in the intestine were not altered by GP-supplementation. However, the bioactive diet significantly increased numbers of eosinophils induced by A. suum infection in the duodenum, jejunum and ileum, and modulated gene expression in the jejunal mucosa of infected pigs. Both GP-supplementation and A. suum infection induced significant and apparently similar changes in the composition of the prokaryotic gut microbiota, and both also decreased concentrations of isobutyric and isovaleric acid (branched-chain short chain fatty acids) in the colon. Our results demonstrate that while a polyphenol-enriched diet in pigs may not directly influence A. suum establishment, it significantly modulates the subsequent host response to helminth infection. Our results suggest an influence of diet on immune function which may potentially be exploited to enhance immunity to helminths. Public Library of Science 2017 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/29966/7/A%20polyphenol-enriched%20diet%20and%20Ascaris.pdf Williams, Andrew R. and Krych, Lukasz and Hajar Fauzan, Ahmad and Nejsum, Peter and Skovgaard, Kerstin and Nielsen, Dennis S. and Thamsborg, Stig M. (2017) A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs. PLoS ONE, 12 (10). pp. 1-21. ISSN 1932-6203. (Published) https://doi.org/10.1371/journal.pone.0186546 https://doi.org/10.1371/journal.pone.0186546 |
| spellingShingle | QL Zoology QR Microbiology QR180 Immunology Williams, Andrew R. Krych, Lukasz Hajar Fauzan, Ahmad Nejsum, Peter Skovgaard, Kerstin Nielsen, Dennis S. Thamsborg, Stig M. A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs |
| title | A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs |
| title_full | A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs |
| title_fullStr | A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs |
| title_full_unstemmed | A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs |
| title_short | A polyphenol-enriched diet and Ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs |
| title_sort | polyphenol-enriched diet and ascaris suum infection modulate mucosal immune responses and gut microbiota composition in pigs |
| topic | QL Zoology QR Microbiology QR180 Immunology |
| url | http://umpir.ump.edu.my/id/eprint/29966/ http://umpir.ump.edu.my/id/eprint/29966/ http://umpir.ump.edu.my/id/eprint/29966/ http://umpir.ump.edu.my/id/eprint/29966/7/A%20polyphenol-enriched%20diet%20and%20Ascaris.pdf |