Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering
Introduction: In search for cross-linkers with multifunctional characteristics, the present work investigated the utility of quaternary ammonium organosilane (QOS) as a potential cross-linker for electrospun collagen nanofibers. We hypothesized that the quaternary ammonium ions improve the electrosp...
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| Format: | Article |
| Language: | English |
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Dovepress
2018
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| Online Access: | http://umpir.ump.edu.my/id/eprint/29827/ http://umpir.ump.edu.my/id/eprint/29827/1/Antimicrobial%20quaternary%20ammonium%20organosilane%20cross-linked%20nanofibrous%20collagen.pdf |
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| author | Dhand, Chetna Balakrishnan, Yamini Ong, Seow Theng Dwivedi, Neeraj Venugopal, Jayarama Reddy Harini, Sriram Leung, Chak Ming Low, Kenny Zhi Wei Loh, Xian Jun Beuerman, Roger W. Ramakrishna, Seeram Verma, Navin Kumar Lakshminarayanan, Rajamani |
| author_facet | Dhand, Chetna Balakrishnan, Yamini Ong, Seow Theng Dwivedi, Neeraj Venugopal, Jayarama Reddy Harini, Sriram Leung, Chak Ming Low, Kenny Zhi Wei Loh, Xian Jun Beuerman, Roger W. Ramakrishna, Seeram Verma, Navin Kumar Lakshminarayanan, Rajamani |
| author_sort | Dhand, Chetna |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Introduction: In search for cross-linkers with multifunctional characteristics, the present work investigated the utility of quaternary ammonium organosilane (QOS) as a potential cross-linker for electrospun collagen nanofibers. We hypothesized that the quaternary ammonium ions improve the electrospinnability by reducing the surface tension and confer antimicrobial properties, while the formation of siloxane after alkaline hydrolysis could cross-link collagen and stimulate cell proliferation. Materials and methods: QOS collagen nanofibers were electrospun by incorporating various concentrations of QOS (0.1%–10% w/w) and were cross-linked in situ after exposure to ammonium carbonate. The QOS cross-linked scaffolds were characterized and their biological properties were evaluated in terms of their biocompatibility, cellular adhesion and metabolic activity for primary human dermal fibroblasts and human fetal osteoblasts. Results and discussion: The study revealed that 1) QOS cross-linking increased the flexibility of otherwise rigid collagen nanofibers and improved the thermal stability; 2) QOS cross-linked mats displayed potent antibacterial activity and 3) the biocompatibility of the composite mats depended on the amount of QOS present in dope solution – at low QOS concentrations (0.1% w/w), the mats promoted mammalian cell proliferation and growth, whereas at higher QOS concentrations, cytotoxic effect was observed. Conclusion: This study demonstrates that QOS cross-linked mats possess anti-infective properties and confer niches for cellular growth and proliferation, thus offering a useful approach, which is important for hard and soft tissue engineering and regenerative medicine. |
| first_indexed | 2025-11-15T02:56:06Z |
| format | Article |
| id | ump-29827 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:56:06Z |
| publishDate | 2018 |
| publisher | Dovepress |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-298272020-11-13T02:50:32Z http://umpir.ump.edu.my/id/eprint/29827/ Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering Dhand, Chetna Balakrishnan, Yamini Ong, Seow Theng Dwivedi, Neeraj Venugopal, Jayarama Reddy Harini, Sriram Leung, Chak Ming Low, Kenny Zhi Wei Loh, Xian Jun Beuerman, Roger W. Ramakrishna, Seeram Verma, Navin Kumar Lakshminarayanan, Rajamani TP Chemical technology Introduction: In search for cross-linkers with multifunctional characteristics, the present work investigated the utility of quaternary ammonium organosilane (QOS) as a potential cross-linker for electrospun collagen nanofibers. We hypothesized that the quaternary ammonium ions improve the electrospinnability by reducing the surface tension and confer antimicrobial properties, while the formation of siloxane after alkaline hydrolysis could cross-link collagen and stimulate cell proliferation. Materials and methods: QOS collagen nanofibers were electrospun by incorporating various concentrations of QOS (0.1%–10% w/w) and were cross-linked in situ after exposure to ammonium carbonate. The QOS cross-linked scaffolds were characterized and their biological properties were evaluated in terms of their biocompatibility, cellular adhesion and metabolic activity for primary human dermal fibroblasts and human fetal osteoblasts. Results and discussion: The study revealed that 1) QOS cross-linking increased the flexibility of otherwise rigid collagen nanofibers and improved the thermal stability; 2) QOS cross-linked mats displayed potent antibacterial activity and 3) the biocompatibility of the composite mats depended on the amount of QOS present in dope solution – at low QOS concentrations (0.1% w/w), the mats promoted mammalian cell proliferation and growth, whereas at higher QOS concentrations, cytotoxic effect was observed. Conclusion: This study demonstrates that QOS cross-linked mats possess anti-infective properties and confer niches for cellular growth and proliferation, thus offering a useful approach, which is important for hard and soft tissue engineering and regenerative medicine. Dovepress 2018-08-03 Article PeerReviewed pdf en cc_by_nc http://umpir.ump.edu.my/id/eprint/29827/1/Antimicrobial%20quaternary%20ammonium%20organosilane%20cross-linked%20nanofibrous%20collagen.pdf Dhand, Chetna and Balakrishnan, Yamini and Ong, Seow Theng and Dwivedi, Neeraj and Venugopal, Jayarama Reddy and Harini, Sriram and Leung, Chak Ming and Low, Kenny Zhi Wei and Loh, Xian Jun and Beuerman, Roger W. and Ramakrishna, Seeram and Verma, Navin Kumar and Lakshminarayanan, Rajamani (2018) Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering. International Journal of Nanomedicine, 13. pp. 4473-4492. ISSN 1178-2013. (Published) https://doi.org/10.2147/IJN.S159770 https://doi.org/10.2147/IJN.S159770 |
| spellingShingle | TP Chemical technology Dhand, Chetna Balakrishnan, Yamini Ong, Seow Theng Dwivedi, Neeraj Venugopal, Jayarama Reddy Harini, Sriram Leung, Chak Ming Low, Kenny Zhi Wei Loh, Xian Jun Beuerman, Roger W. Ramakrishna, Seeram Verma, Navin Kumar Lakshminarayanan, Rajamani Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title | Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title_full | Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title_fullStr | Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title_full_unstemmed | Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title_short | Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title_sort | antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/29827/ http://umpir.ump.edu.my/id/eprint/29827/ http://umpir.ump.edu.my/id/eprint/29827/ http://umpir.ump.edu.my/id/eprint/29827/1/Antimicrobial%20quaternary%20ammonium%20organosilane%20cross-linked%20nanofibrous%20collagen.pdf |