Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin

A 5 mg/mL solution of water, methanol and acetone extracts of seaweeds were used for α-glucosidase inhibition assay hyphenated with high performance liquid chromatography–mass spectrometry (HPLC–HRMS). The results showed acetone extracts of Undaria pinnatifida has the strongest inhibitory effect aga...

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Main Authors: Nazikussabah, Zaharudin, Staerk, Dan, Dragsted, Lars Ove
Format: Article
Language:English
Published: Elsevier Ltd 2019
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/29668/
http://umpir.ump.edu.my/id/eprint/29668/7/Inhibition%20of%20aglucosidase.pdf
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author Nazikussabah, Zaharudin
Staerk, Dan
Dragsted, Lars Ove
author_facet Nazikussabah, Zaharudin
Staerk, Dan
Dragsted, Lars Ove
author_sort Nazikussabah, Zaharudin
building UMP Institutional Repository
collection Online Access
description A 5 mg/mL solution of water, methanol and acetone extracts of seaweeds were used for α-glucosidase inhibition assay hyphenated with high performance liquid chromatography–mass spectrometry (HPLC–HRMS). The results showed acetone extracts of Undaria pinnatifida has the strongest inhibitory effect against α-glucosidase activity with IC50 0.08 ± 0.002 mg/mL. The active compound found in Undaria pinnatifida was identified as fucoxanthin. Analytical standard sample of fucoxanthin significantly inhibited α-glucosidase with IC50 value 0.047 ± 0.001 mg/mL. An inhibition kinetics study indicates that fucoxanthin is showing mixed-type inhibition. These results suggest that Undaria pinnatifida has a potential to inhibit α-glucosidase and may be used as a bioactive food ingredient for glycaemic control.
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publishDate 2019
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spelling ump-296682020-11-19T01:46:12Z http://umpir.ump.edu.my/id/eprint/29668/ Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin Nazikussabah, Zaharudin Staerk, Dan Dragsted, Lars Ove QD Chemistry A 5 mg/mL solution of water, methanol and acetone extracts of seaweeds were used for α-glucosidase inhibition assay hyphenated with high performance liquid chromatography–mass spectrometry (HPLC–HRMS). The results showed acetone extracts of Undaria pinnatifida has the strongest inhibitory effect against α-glucosidase activity with IC50 0.08 ± 0.002 mg/mL. The active compound found in Undaria pinnatifida was identified as fucoxanthin. Analytical standard sample of fucoxanthin significantly inhibited α-glucosidase with IC50 value 0.047 ± 0.001 mg/mL. An inhibition kinetics study indicates that fucoxanthin is showing mixed-type inhibition. These results suggest that Undaria pinnatifida has a potential to inhibit α-glucosidase and may be used as a bioactive food ingredient for glycaemic control. Elsevier Ltd 2019-01-01 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/29668/7/Inhibition%20of%20aglucosidase.pdf Nazikussabah, Zaharudin and Staerk, Dan and Dragsted, Lars Ove (2019) Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin. Food Chemistry, 270. pp. 481-486. ISSN 0308-8146. (Published) https://doi.org/10.1016/j.foodchem.2018.07.142 https://doi.org/10.1016/j.foodchem.2018.07.142
spellingShingle QD Chemistry
Nazikussabah, Zaharudin
Staerk, Dan
Dragsted, Lars Ove
Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin
title Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin
title_full Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin
title_fullStr Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin
title_full_unstemmed Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin
title_short Inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin
title_sort inhibition of α-glucosidase activity by selected edible seaweeds and fucoxanthin
topic QD Chemistry
url http://umpir.ump.edu.my/id/eprint/29668/
http://umpir.ump.edu.my/id/eprint/29668/
http://umpir.ump.edu.my/id/eprint/29668/
http://umpir.ump.edu.my/id/eprint/29668/7/Inhibition%20of%20aglucosidase.pdf