D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation
Production ᴅ-xylonic acid from recombinant E. coli BL21 (DE3) at a small scale has been successfully done several times. In an attempt to scale up the production process to a bigger scale, fermentation in bioreactor was conducted. The comparison on growth of recombinant E. coli BL21 (DE3) and produc...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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IOP Publishing
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/29359/ http://umpir.ump.edu.my/id/eprint/29359/1/26.%20D-Xylonic%20acid%20from%20recombinant%20e.%20coli%20BL21%20%28DE3%29.pdf |
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| author | Nurul Atikah, Mohd Rodzri Salwani, Md Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah R. M., Illias |
| author_facet | Nurul Atikah, Mohd Rodzri Salwani, Md Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah R. M., Illias |
| author_sort | Nurul Atikah, Mohd Rodzri |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Production ᴅ-xylonic acid from recombinant E. coli BL21 (DE3) at a small scale has been successfully done several times. In an attempt to scale up the production process to a bigger scale, fermentation in bioreactor was conducted. The comparison on growth of recombinant E. coli BL21 (DE3) and production of ᴅ-xylonic acid in both shake flask and bioreactor was investigated. Higher specific growth rate of recombinant E. coli BL21 (DE3) and concentration of ᴅ-xylonic acid however, was obtained by fermentation in shake flask which is 0.273 h-1 and 9.06 gL-1 ᴅ-xylonic acid respectively. The result suggests several mistakes done during fermentation in bioreactor that causes low concentration of ᴅ-xylonic acid in bioreactor fermentation. Therefore, this calls up for improvement and better scale-up strategies in future work. |
| first_indexed | 2025-11-15T02:54:20Z |
| format | Conference or Workshop Item |
| id | ump-29359 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:54:20Z |
| publishDate | 2020 |
| publisher | IOP Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-293592021-02-09T08:48:55Z http://umpir.ump.edu.my/id/eprint/29359/ D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation Nurul Atikah, Mohd Rodzri Salwani, Md Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah R. M., Illias TP Chemical technology Production ᴅ-xylonic acid from recombinant E. coli BL21 (DE3) at a small scale has been successfully done several times. In an attempt to scale up the production process to a bigger scale, fermentation in bioreactor was conducted. The comparison on growth of recombinant E. coli BL21 (DE3) and production of ᴅ-xylonic acid in both shake flask and bioreactor was investigated. Higher specific growth rate of recombinant E. coli BL21 (DE3) and concentration of ᴅ-xylonic acid however, was obtained by fermentation in shake flask which is 0.273 h-1 and 9.06 gL-1 ᴅ-xylonic acid respectively. The result suggests several mistakes done during fermentation in bioreactor that causes low concentration of ᴅ-xylonic acid in bioreactor fermentation. Therefore, this calls up for improvement and better scale-up strategies in future work. IOP Publishing 2020 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/29359/1/26.%20D-Xylonic%20acid%20from%20recombinant%20e.%20coli%20BL21%20%28DE3%29.pdf Nurul Atikah, Mohd Rodzri and Salwani, Md Zain and Rafidah, Mohamed Ali Hanapiah and Rozaimi, Abu Samah and R. M., Illias (2020) D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation. In: Journal of Physics: Conference Series, The 2nd Joint International Conference on Emerging Computing Technology and Sports (JICETS 2019) , 25-27 November 2019 , Bandung, Indonesia. pp. 1-8., 1529 (052073). ISSN 1742-6588 (print); 1742-6596 (online) (Published) |
| spellingShingle | TP Chemical technology Nurul Atikah, Mohd Rodzri Salwani, Md Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah R. M., Illias D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation |
| title | D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation |
| title_full | D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation |
| title_fullStr | D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation |
| title_full_unstemmed | D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation |
| title_short | D-Xylonic acid from recombinant e. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation |
| title_sort | d-xylonic acid from recombinant e. coli bl21 (de3): comparison between shake flask and benchtop bioreactor fermentation |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/29359/ http://umpir.ump.edu.my/id/eprint/29359/1/26.%20D-Xylonic%20acid%20from%20recombinant%20e.%20coli%20BL21%20%28DE3%29.pdf |