Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology
The application of the xylose reductase (XR) enzyme in the development of biotechnology demands an efficient and large scale enzyme separation technique. The aim of this present work was to optimize xylose reductase (XR) purification process through ultrafiltration membrane (UF) technology using Cen...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier B.V
2020
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| Online Access: | https://umpir.ump.edu.my/id/eprint/29222/ |
| _version_ | 1848827271468548096 |
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| author | Santhana, Krishnan Noor Suzana, Bakar Zularisam, Abdul Wahid Mohd Nasrullah, Zulkifli Mimi Sakinah, Abdul Munaim Mohd Fadhil, Md Din Shazwin, Mat Taib You Li, Yu |
| author_facet | Santhana, Krishnan Noor Suzana, Bakar Zularisam, Abdul Wahid Mohd Nasrullah, Zulkifli Mimi Sakinah, Abdul Munaim Mohd Fadhil, Md Din Shazwin, Mat Taib You Li, Yu |
| author_sort | Santhana, Krishnan |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The application of the xylose reductase (XR) enzyme in the development of biotechnology demands an efficient and large scale enzyme separation technique. The aim of this present work was to optimize xylose reductase (XR) purification process through ultrafiltration membrane (UF) technology using Central composite design (CCD) of response surface methods (RSM). The three effective parameters analyzed were filtration time (0–100), transmembrane pressure (TMP) (1–1.6 bar), cross flow velocity (CFV) (0.52–1.2 cm/ s�1) and its combined effect to obtain high flux with less possibility of membrane fouling. Experimental studies revealed that the best range for optimization process for filtration time, operational transmembrane pressure and cross flow velocity was 30 min,1.4 bars and 1.06 cm/s, respectively as these conditions yielded the highest membrane permeability (56.03 Lm-2h-1 bar�1) and xylitol content (15.49 g/l). According to the analysis of variance (ANOVA), the p-value (<0.0001) indicated the designed model was highly significant. The errorpercentage etweentheactualandpredictedvalueformembranepermeabilityandxylitolamount
(2.21 % and 4.85 % respectively), which both were found to be close to the predicted values. The verification experiments gave membrane actual permeability of 57.3 Lm-2h-1 bar�1 and 16.29 g/l of xylitol production, thus indicating that the successfully developed model to predict the response. |
| first_indexed | 2025-11-15T03:58:04Z |
| format | Article |
| id | ump-29222 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T03:58:04Z |
| publishDate | 2020 |
| publisher | Elsevier B.V |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-292222025-09-25T05:09:52Z https://umpir.ump.edu.my/id/eprint/29222/ Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology Santhana, Krishnan Noor Suzana, Bakar Zularisam, Abdul Wahid Mohd Nasrullah, Zulkifli Mimi Sakinah, Abdul Munaim Mohd Fadhil, Md Din Shazwin, Mat Taib You Li, Yu TA Engineering (General). Civil engineering (General) TG Bridge engineering TP Chemical technology The application of the xylose reductase (XR) enzyme in the development of biotechnology demands an efficient and large scale enzyme separation technique. The aim of this present work was to optimize xylose reductase (XR) purification process through ultrafiltration membrane (UF) technology using Central composite design (CCD) of response surface methods (RSM). The three effective parameters analyzed were filtration time (0–100), transmembrane pressure (TMP) (1–1.6 bar), cross flow velocity (CFV) (0.52–1.2 cm/ s�1) and its combined effect to obtain high flux with less possibility of membrane fouling. Experimental studies revealed that the best range for optimization process for filtration time, operational transmembrane pressure and cross flow velocity was 30 min,1.4 bars and 1.06 cm/s, respectively as these conditions yielded the highest membrane permeability (56.03 Lm-2h-1 bar�1) and xylitol content (15.49 g/l). According to the analysis of variance (ANOVA), the p-value (<0.0001) indicated the designed model was highly significant. The errorpercentage etweentheactualandpredictedvalueformembranepermeabilityandxylitolamount (2.21 % and 4.85 % respectively), which both were found to be close to the predicted values. The verification experiments gave membrane actual permeability of 57.3 Lm-2h-1 bar�1 and 16.29 g/l of xylitol production, thus indicating that the successfully developed model to predict the response. Elsevier B.V 2020 Article PeerReviewed pdf en cc_by_nc_nd_4 https://umpir.ump.edu.my/id/eprint/29222/1/Optimization%20of%20operating%20parameters%20for%20xylose%20reductase.pdf Santhana, Krishnan and Noor Suzana, Bakar and Zularisam, Abdul Wahid and Mohd Nasrullah, Zulkifli and Mimi Sakinah, Abdul Munaim and Mohd Fadhil, Md Din and Shazwin, Mat Taib and You Li, Yu (2020) Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology. Biotechnology Reports, 27 (e00498). pp. 1-11. ISSN 2215-017X. (Published) https://doi.org/10.1016/j.btre.2020.e00498 DOI: https://doi.org/10.1016/j.btre.2020.e00498 DOI: https://doi.org/10.1016/j.btre.2020.e00498 |
| spellingShingle | TA Engineering (General). Civil engineering (General) TG Bridge engineering TP Chemical technology Santhana, Krishnan Noor Suzana, Bakar Zularisam, Abdul Wahid Mohd Nasrullah, Zulkifli Mimi Sakinah, Abdul Munaim Mohd Fadhil, Md Din Shazwin, Mat Taib You Li, Yu Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology |
| title | Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology |
| title_full | Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology |
| title_fullStr | Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology |
| title_full_unstemmed | Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology |
| title_short | Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology |
| title_sort | optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology |
| topic | TA Engineering (General). Civil engineering (General) TG Bridge engineering TP Chemical technology |
| url | https://umpir.ump.edu.my/id/eprint/29222/ https://umpir.ump.edu.my/id/eprint/29222/ https://umpir.ump.edu.my/id/eprint/29222/ |