Extraction and identification of bioactive compounds from L. pumila

This paper presents the comparison of extraction methods between maceration extraction (ME) and ultrasonic assisted extarction (UAE) to the bioactive compounds yield (gallic acid, protocatechuic acid, epigallocatechin and rutin) of Labisia pumila. A grinded dried plant material with size ranging 246...

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Main Authors: Afiqah, Yeop, Jessinta, Sandanasamy, Pang, Sook Fun, Sureena, Abdullah, Mashitah, Mohd Yusoff, Jolius, Gimbun
Format: Article
Language:English
Published: RMP Publications 2019
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/29170/
http://umpir.ump.edu.my/id/eprint/29170/1/30012019-jesr-01-05.pdf
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author Afiqah, Yeop
Jessinta, Sandanasamy
Pang, Sook Fun
Sureena, Abdullah
Mashitah, Mohd Yusoff
Jolius, Gimbun
author_facet Afiqah, Yeop
Jessinta, Sandanasamy
Pang, Sook Fun
Sureena, Abdullah
Mashitah, Mohd Yusoff
Jolius, Gimbun
author_sort Afiqah, Yeop
building UMP Institutional Repository
collection Online Access
description This paper presents the comparison of extraction methods between maceration extraction (ME) and ultrasonic assisted extarction (UAE) to the bioactive compounds yield (gallic acid, protocatechuic acid, epigallocatechin and rutin) of Labisia pumila. A grinded dried plant material with size ranging 246.58μm to 257.72μm was performed throughout this work. The gallic acid, protoctechuic acid, epigallocatechin and rutin qualification and quantification were performed using an ultra-performance liquid chromatography coupled photodiode array (UPLC-PDA). Exact match between the residence time from the plant extract and external standard was found indicating a presence of these four targeted bioactive compounds. It was found that UAE method has the highest extraction yield; gallic acid (0.0293 mg GA/g DW), protocatechuic acid (0.0081 mg PCA/g DW), epigallocatechin (0.0057 mg EGC/g DW) and rutin (0.0038 mg Rutin/g DW) compared to ME. The findings in this work may serve as a useful guide to obtain a highest extraction yield of these four targeted bioactive compounds from L. pumila.
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spelling ump-291702022-08-23T01:11:06Z http://umpir.ump.edu.my/id/eprint/29170/ Extraction and identification of bioactive compounds from L. pumila Afiqah, Yeop Jessinta, Sandanasamy Pang, Sook Fun Sureena, Abdullah Mashitah, Mohd Yusoff Jolius, Gimbun TP Chemical technology This paper presents the comparison of extraction methods between maceration extraction (ME) and ultrasonic assisted extarction (UAE) to the bioactive compounds yield (gallic acid, protocatechuic acid, epigallocatechin and rutin) of Labisia pumila. A grinded dried plant material with size ranging 246.58μm to 257.72μm was performed throughout this work. The gallic acid, protoctechuic acid, epigallocatechin and rutin qualification and quantification were performed using an ultra-performance liquid chromatography coupled photodiode array (UPLC-PDA). Exact match between the residence time from the plant extract and external standard was found indicating a presence of these four targeted bioactive compounds. It was found that UAE method has the highest extraction yield; gallic acid (0.0293 mg GA/g DW), protocatechuic acid (0.0081 mg PCA/g DW), epigallocatechin (0.0057 mg EGC/g DW) and rutin (0.0038 mg Rutin/g DW) compared to ME. The findings in this work may serve as a useful guide to obtain a highest extraction yield of these four targeted bioactive compounds from L. pumila. RMP Publications 2019 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/29170/1/30012019-jesr-01-05.pdf Afiqah, Yeop and Jessinta, Sandanasamy and Pang, Sook Fun and Sureena, Abdullah and Mashitah, Mohd Yusoff and Jolius, Gimbun (2019) Extraction and identification of bioactive compounds from L. pumila. Journal of Engineering and Science Research, 3 (3). pp. 1-5. ISSN 2289-7127. (Published) https://doi.org/10.26666/rmp.jesr.2019.3.1 https://doi.org/10.26666/rmp.jesr.2019.3.1
spellingShingle TP Chemical technology
Afiqah, Yeop
Jessinta, Sandanasamy
Pang, Sook Fun
Sureena, Abdullah
Mashitah, Mohd Yusoff
Jolius, Gimbun
Extraction and identification of bioactive compounds from L. pumila
title Extraction and identification of bioactive compounds from L. pumila
title_full Extraction and identification of bioactive compounds from L. pumila
title_fullStr Extraction and identification of bioactive compounds from L. pumila
title_full_unstemmed Extraction and identification of bioactive compounds from L. pumila
title_short Extraction and identification of bioactive compounds from L. pumila
title_sort extraction and identification of bioactive compounds from l. pumila
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/29170/
http://umpir.ump.edu.my/id/eprint/29170/
http://umpir.ump.edu.my/id/eprint/29170/
http://umpir.ump.edu.my/id/eprint/29170/1/30012019-jesr-01-05.pdf