Evaluation of factors for cells growth of immobilized Chlorella vulgaris via factorial design approach

Immobilization technique is used to simplify the separation process of microalgae from the culture medium. Statistical design of factors influencing the number of cells growth and cells loss of immobilized Chlorella vulgaris for lipid production was attempted by using factorial design approach. Five...

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Main Authors: Noor Raihana, Abu Sepian, Nur Hidayah, Mat Yasin, Norazwina, Zainol
Format: Article
Language:English
English
Published: Taylor & Francis 2021
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/28911/
http://umpir.ump.edu.my/id/eprint/28911/1/Evaluation%20of%20factors%20for%20cells%20growth%20of%20immobilized%20Chlorella1.pdf
http://umpir.ump.edu.my/id/eprint/28911/7/Evaluation%20of%20factors%20for%20cells%20growth%20of%20immobilized%20Chlorella%20vulgaris%20via%20factorial%20design%20approach.pdf
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author Noor Raihana, Abu Sepian
Nur Hidayah, Mat Yasin
Norazwina, Zainol
author_facet Noor Raihana, Abu Sepian
Nur Hidayah, Mat Yasin
Norazwina, Zainol
author_sort Noor Raihana, Abu Sepian
building UMP Institutional Repository
collection Online Access
description Immobilization technique is used to simplify the separation process of microalgae from the culture medium. Statistical design of factors influencing the number of cells growth and cells loss of immobilized Chlorella vulgaris for lipid production was attempted by using factorial design approach. Five factors including photoperiod, cultivation period, concentration of glucose, concentration of NaNO3, and concentration of CaCl2 were selected and screened using fractional factorial design (FFD). Based on the 16 experiments generated in FFD, photoperiod and cultivation days were identified as the significant factors for both responses. The maximum number of cells growth (2.32 × 109 cells/mL) and minimum number of cells loss (1.52 × 104 cells/mL) were obtained from the suggested conditions of 0.01 g/L of CaCl2, 5.03 g/L of NaNO3, 24 h of photoperiod, 8 cultivation days, and 23.97 g/L of glucose. The results indicate that FFD is a useful tool to determine the significant factors for immobilized cells growth by considering all the factors involved and increased the lipid production by 17.27%. Furthermore, this study revealed the important factors for successful immobilization of microalgae which is useful for biofuel application.
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spelling ump-289112022-04-28T07:11:33Z http://umpir.ump.edu.my/id/eprint/28911/ Evaluation of factors for cells growth of immobilized Chlorella vulgaris via factorial design approach Noor Raihana, Abu Sepian Nur Hidayah, Mat Yasin Norazwina, Zainol TP Chemical technology Immobilization technique is used to simplify the separation process of microalgae from the culture medium. Statistical design of factors influencing the number of cells growth and cells loss of immobilized Chlorella vulgaris for lipid production was attempted by using factorial design approach. Five factors including photoperiod, cultivation period, concentration of glucose, concentration of NaNO3, and concentration of CaCl2 were selected and screened using fractional factorial design (FFD). Based on the 16 experiments generated in FFD, photoperiod and cultivation days were identified as the significant factors for both responses. The maximum number of cells growth (2.32 × 109 cells/mL) and minimum number of cells loss (1.52 × 104 cells/mL) were obtained from the suggested conditions of 0.01 g/L of CaCl2, 5.03 g/L of NaNO3, 24 h of photoperiod, 8 cultivation days, and 23.97 g/L of glucose. The results indicate that FFD is a useful tool to determine the significant factors for immobilized cells growth by considering all the factors involved and increased the lipid production by 17.27%. Furthermore, this study revealed the important factors for successful immobilization of microalgae which is useful for biofuel application. Taylor & Francis 2021 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/28911/1/Evaluation%20of%20factors%20for%20cells%20growth%20of%20immobilized%20Chlorella1.pdf pdf en http://umpir.ump.edu.my/id/eprint/28911/7/Evaluation%20of%20factors%20for%20cells%20growth%20of%20immobilized%20Chlorella%20vulgaris%20via%20factorial%20design%20approach.pdf Noor Raihana, Abu Sepian and Nur Hidayah, Mat Yasin and Norazwina, Zainol (2021) Evaluation of factors for cells growth of immobilized Chlorella vulgaris via factorial design approach. Chemical Engineering Communications, 208 (5). pp. 601-612. ISSN 1563-5201. (Published) https://doi.org/10.1080/00986445.2019.1680372 https://doi.org/10.1080/00986445.2019.1680372
spellingShingle TP Chemical technology
Noor Raihana, Abu Sepian
Nur Hidayah, Mat Yasin
Norazwina, Zainol
Evaluation of factors for cells growth of immobilized Chlorella vulgaris via factorial design approach
title Evaluation of factors for cells growth of immobilized Chlorella vulgaris via factorial design approach
title_full Evaluation of factors for cells growth of immobilized Chlorella vulgaris via factorial design approach
title_fullStr Evaluation of factors for cells growth of immobilized Chlorella vulgaris via factorial design approach
title_full_unstemmed Evaluation of factors for cells growth of immobilized Chlorella vulgaris via factorial design approach
title_short Evaluation of factors for cells growth of immobilized Chlorella vulgaris via factorial design approach
title_sort evaluation of factors for cells growth of immobilized chlorella vulgaris via factorial design approach
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/28911/
http://umpir.ump.edu.my/id/eprint/28911/
http://umpir.ump.edu.my/id/eprint/28911/
http://umpir.ump.edu.my/id/eprint/28911/1/Evaluation%20of%20factors%20for%20cells%20growth%20of%20immobilized%20Chlorella1.pdf
http://umpir.ump.edu.my/id/eprint/28911/7/Evaluation%20of%20factors%20for%20cells%20growth%20of%20immobilized%20Chlorella%20vulgaris%20via%20factorial%20design%20approach.pdf