Mass spectrometry analysis of auxiliary energy-induced terpenes extraction from Andrographis Paniculata

The auxiliary energy level affects the yield of terpene extraction from Andrographis paniculata. This paper presents the effect of auxiliary energy (0.22–50 W/mL) to the extraction yield of terpenes from Andrographis paniculata for the first time. Ultra-performance liquid chromatography-quadrupole t...

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Main Authors: Chia, Vi Vien, Pang, Sook Fun, Jolius, Gimbun
Format: Article
Published: Elsevier Ltd 2020
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/28902/
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author Chia, Vi Vien
Pang, Sook Fun
Jolius, Gimbun
author_facet Chia, Vi Vien
Pang, Sook Fun
Jolius, Gimbun
author_sort Chia, Vi Vien
building UMP Institutional Repository
collection Online Access
description The auxiliary energy level affects the yield of terpene extraction from Andrographis paniculata. This paper presents the effect of auxiliary energy (0.22–50 W/mL) to the extraction yield of terpenes from Andrographis paniculata for the first time. Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) method was developed for qualitative and quantitative analysis of terpene. It was found that, the highest yield of andrographolide (20.10 mg g/DW) and neoandrographolide (9.54 mg g/DW) was produced at a relatively lower level of auxiliary energy (1.34 W/mL). Meanwhile the highest yield of 14-deoxy-11,12-didehydroandrographolide (6.47 mg g/DW) was obtained at a higher level of auxiliary energy (25 W/mL), which indicates the existence of a thermally-induced extractive-synthesis mechanism. Principal component analyses on the mass spectra confirmed maceration, ultrasonic, and microwave assisted extraction differ appreciably and eight compounds contributing to the differences were successfully elucidated.
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spelling ump-289022021-04-22T06:48:33Z http://umpir.ump.edu.my/id/eprint/28902/ Mass spectrometry analysis of auxiliary energy-induced terpenes extraction from Andrographis Paniculata Chia, Vi Vien Pang, Sook Fun Jolius, Gimbun TP Chemical technology The auxiliary energy level affects the yield of terpene extraction from Andrographis paniculata. This paper presents the effect of auxiliary energy (0.22–50 W/mL) to the extraction yield of terpenes from Andrographis paniculata for the first time. Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) method was developed for qualitative and quantitative analysis of terpene. It was found that, the highest yield of andrographolide (20.10 mg g/DW) and neoandrographolide (9.54 mg g/DW) was produced at a relatively lower level of auxiliary energy (1.34 W/mL). Meanwhile the highest yield of 14-deoxy-11,12-didehydroandrographolide (6.47 mg g/DW) was obtained at a higher level of auxiliary energy (25 W/mL), which indicates the existence of a thermally-induced extractive-synthesis mechanism. Principal component analyses on the mass spectra confirmed maceration, ultrasonic, and microwave assisted extraction differ appreciably and eight compounds contributing to the differences were successfully elucidated. Elsevier Ltd 2020-07-28 Article PeerReviewed Chia, Vi Vien and Pang, Sook Fun and Jolius, Gimbun (2020) Mass spectrometry analysis of auxiliary energy-induced terpenes extraction from Andrographis Paniculata. Industrial Crops and Products, 155 (112828). pp. 1-7. ISSN 0926-6690. (Published) https://doi.org/10.1016/j.indcrop.2020.112828 https://doi.org/10.1016/j.indcrop.2020.112828
spellingShingle TP Chemical technology
Chia, Vi Vien
Pang, Sook Fun
Jolius, Gimbun
Mass spectrometry analysis of auxiliary energy-induced terpenes extraction from Andrographis Paniculata
title Mass spectrometry analysis of auxiliary energy-induced terpenes extraction from Andrographis Paniculata
title_full Mass spectrometry analysis of auxiliary energy-induced terpenes extraction from Andrographis Paniculata
title_fullStr Mass spectrometry analysis of auxiliary energy-induced terpenes extraction from Andrographis Paniculata
title_full_unstemmed Mass spectrometry analysis of auxiliary energy-induced terpenes extraction from Andrographis Paniculata
title_short Mass spectrometry analysis of auxiliary energy-induced terpenes extraction from Andrographis Paniculata
title_sort mass spectrometry analysis of auxiliary energy-induced terpenes extraction from andrographis paniculata
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/28902/
http://umpir.ump.edu.my/id/eprint/28902/
http://umpir.ump.edu.my/id/eprint/28902/