Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production
Enzymatic hydrolysis has become outstanding technology in converting lignocellulosic biomass to its xylose monomer for xylooligosaccharides (XOS) production. The present work involves an investigation on the effects of enzyme loading, agitation speed, substrate loading, temperature and hydrolysis ti...
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| Format: | Article |
| Language: | English |
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Asian Journal of Agriculture and Biology
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/28690/ http://umpir.ump.edu.my/id/eprint/28690/1/27-Aishah%20-%20AJAB_2019.pdf |
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| author | Anis Suriyani, Mat Yaacob Nurul Aishah, Mazlan Kamaliah, Abdul Samad Syed Mohd Saufi, Tuan Chik Hafizuddin, Wan Yussof |
| author_facet | Anis Suriyani, Mat Yaacob Nurul Aishah, Mazlan Kamaliah, Abdul Samad Syed Mohd Saufi, Tuan Chik Hafizuddin, Wan Yussof |
| author_sort | Anis Suriyani, Mat Yaacob |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Enzymatic hydrolysis has become outstanding technology in converting lignocellulosic biomass to its xylose monomer for xylooligosaccharides (XOS) production. The present work involves an investigation on the effects of enzyme loading, agitation speed, substrate loading, temperature and hydrolysis time on enzymatic hydrolysis for XOS production. Pretreated oil palm frond bagasse by dilute nitric acid was used for enzymatic hydrolysis using Cellic HTec2. The effects of factors were analyzed by half fractional factorial design 25-1 using Design Expert with Response Surface Methodology (RSM) to achieve maximum XOS production. The results revealed that the best enzymatic hydrolysis condition yielded 4.13 mg/L of XOS when conducted at 5% (w/v) of substrate loading, 50 U/mL enzyme loading with 200 rpm agitation speed and 55°C for 4 hours of hydrolysis time. Two factors that contributed to the highest production of XOS were substrate loading and enzyme loading. The model obtained in this present research is significant with p-value < 0.0001 and R-squared of 0.9545. It is recommended that model had a maximum point which is possible for the optimization process later. Overall, the findings of this study suggest that Cellic HTec2 is a suitable candidate for enzymatic hydrolysis of pretreated OPFB for higher XOS production. |
| first_indexed | 2025-11-15T02:52:00Z |
| format | Article |
| id | ump-28690 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:52:00Z |
| publishDate | 2019 |
| publisher | Asian Journal of Agriculture and Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-286902020-07-10T07:32:21Z http://umpir.ump.edu.my/id/eprint/28690/ Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production Anis Suriyani, Mat Yaacob Nurul Aishah, Mazlan Kamaliah, Abdul Samad Syed Mohd Saufi, Tuan Chik Hafizuddin, Wan Yussof TP Chemical technology Enzymatic hydrolysis has become outstanding technology in converting lignocellulosic biomass to its xylose monomer for xylooligosaccharides (XOS) production. The present work involves an investigation on the effects of enzyme loading, agitation speed, substrate loading, temperature and hydrolysis time on enzymatic hydrolysis for XOS production. Pretreated oil palm frond bagasse by dilute nitric acid was used for enzymatic hydrolysis using Cellic HTec2. The effects of factors were analyzed by half fractional factorial design 25-1 using Design Expert with Response Surface Methodology (RSM) to achieve maximum XOS production. The results revealed that the best enzymatic hydrolysis condition yielded 4.13 mg/L of XOS when conducted at 5% (w/v) of substrate loading, 50 U/mL enzyme loading with 200 rpm agitation speed and 55°C for 4 hours of hydrolysis time. Two factors that contributed to the highest production of XOS were substrate loading and enzyme loading. The model obtained in this present research is significant with p-value < 0.0001 and R-squared of 0.9545. It is recommended that model had a maximum point which is possible for the optimization process later. Overall, the findings of this study suggest that Cellic HTec2 is a suitable candidate for enzymatic hydrolysis of pretreated OPFB for higher XOS production. Asian Journal of Agriculture and Biology 2019 Article PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/28690/1/27-Aishah%20-%20AJAB_2019.pdf Anis Suriyani, Mat Yaacob and Nurul Aishah, Mazlan and Kamaliah, Abdul Samad and Syed Mohd Saufi, Tuan Chik and Hafizuddin, Wan Yussof (2019) Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production. Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production, 7 (1). pp. 122-129. ISSN 23078553. (Published) https://pdfs.semanticscholar.org/8c0c/45929bb78ee371beb9476a56c38ac9bcd9d0.pdf https://www.asianjab.com/wp-content/uploads/2019/04/14.AJAB-P57_122-129.pdf |
| spellingShingle | TP Chemical technology Anis Suriyani, Mat Yaacob Nurul Aishah, Mazlan Kamaliah, Abdul Samad Syed Mohd Saufi, Tuan Chik Hafizuddin, Wan Yussof Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production |
| title | Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production |
| title_full | Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production |
| title_fullStr | Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production |
| title_full_unstemmed | Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production |
| title_short | Factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic HTec2 for xylooligosaccharides production |
| title_sort | factors affecting enzymatic hydrolysis of oil palm frond bagasse using cellic htec2 for xylooligosaccharides production |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/28690/ http://umpir.ump.edu.my/id/eprint/28690/ http://umpir.ump.edu.my/id/eprint/28690/ http://umpir.ump.edu.my/id/eprint/28690/1/27-Aishah%20-%20AJAB_2019.pdf |