Synthesis and antiparkinsonian activity of nanocomposite of chitosan-tripolyphosphate-Mucuna pruriens L extract (CS-TPP-MP)
Mucuna pruriens L. (MP) has antiparkinsonian activity because it contains levodopa that acts as a dopamine precursor and plays a role to stimulate dopaminergic receptors in Parkinson's sufferers. The therapeutic efficacy of MP extract can be improved by using a nano drug carrier system, such as...
| Main Authors: | , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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IOP Publishing
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/28586/ http://umpir.ump.edu.my/id/eprint/28586/1/Synthesis%20and%20antiparkinsonian%20activity%20of%20nanocomposite%20of%20chitosan.pdf |
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| author | Sardjono, R. E. Fauziyah, A. N. Puspitasari, M. D. Musthapa, I. Khoerunnisa, F. Azzahra, G. N. R., Mamat Erdiwansyah, . |
| author_facet | Sardjono, R. E. Fauziyah, A. N. Puspitasari, M. D. Musthapa, I. Khoerunnisa, F. Azzahra, G. N. R., Mamat Erdiwansyah, . |
| author_sort | Sardjono, R. E. |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Mucuna pruriens L. (MP) has antiparkinsonian activity because it contains levodopa that acts as a dopamine precursor and plays a role to stimulate dopaminergic receptors in Parkinson's sufferers. The therapeutic efficacy of MP extract can be improved by using a nano drug carrier system, such as chitosan-tripolyphosphate (CS-TPP). This study aims to synthesize, characterize, and evaluate antiparkinsonian activity of chitosan-tripolyphosphate-MP extract (CS-TPP-MP) nanocomposite in mice. MP seed powder was extracted by maceration method using water-ethanol (1:1) by adding citric acid until it reached pH 3. The CS-TPP-MP nanocomposite was synthesized by using ionic gelation method with variations in reactant composition and reaction time. The CS-TPP-MP nanocomposite was characterized by Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX), X-Ray Diffraction (XRD) and Fourier-Transform Infrared (FTIR) Spectroscopy. Catalepsy test was performed to find the antiparkinsonian activity level of CS-TPP-MP nanocomposite at doses of 5, 10, 15, 20, and 25 mg/kg body weight. Based on the results of CS-TPP-MP synthesis, it was found that the reactant composition (CS-TPP:MP) of 1:3 with reaction time of 20 minutes produced the highest yield (14.21%.) SEM-EDX characterization showed that the morphology of CS-TPP-MP nanocomposite was predominantly spherical and the size was approximately 120-170 nm with a composition of C = 55.43%, O = 30.46%, N = 13.46%, P = 0.44%. XRD diffractogram showed that CS-TPP-MP nanocomposite has amorphous structure. FTIR analysis showed the appearance of absorption at wavelength of 1643.35 cm−1 which proved the interaction between the primary amine group of chitosan and the carbonyl group of EMP. Catalepsy test demonstrated that CS-TPP-MP nanocomposite at the doses of 5, 10, 15, 20, and 25 mg/kg body weight could reduce catalepsy symptoms in mice significantly, and the best dosage was 20 mg/kg body weight. |
| first_indexed | 2025-11-15T02:51:37Z |
| format | Conference or Workshop Item |
| id | ump-28586 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:51:37Z |
| publishDate | 2020 |
| publisher | IOP Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-285862021-02-08T08:42:03Z http://umpir.ump.edu.my/id/eprint/28586/ Synthesis and antiparkinsonian activity of nanocomposite of chitosan-tripolyphosphate-Mucuna pruriens L extract (CS-TPP-MP) Sardjono, R. E. Fauziyah, A. N. Puspitasari, M. D. Musthapa, I. Khoerunnisa, F. Azzahra, G. N. R., Mamat Erdiwansyah, . RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry TP Chemical technology Mucuna pruriens L. (MP) has antiparkinsonian activity because it contains levodopa that acts as a dopamine precursor and plays a role to stimulate dopaminergic receptors in Parkinson's sufferers. The therapeutic efficacy of MP extract can be improved by using a nano drug carrier system, such as chitosan-tripolyphosphate (CS-TPP). This study aims to synthesize, characterize, and evaluate antiparkinsonian activity of chitosan-tripolyphosphate-MP extract (CS-TPP-MP) nanocomposite in mice. MP seed powder was extracted by maceration method using water-ethanol (1:1) by adding citric acid until it reached pH 3. The CS-TPP-MP nanocomposite was synthesized by using ionic gelation method with variations in reactant composition and reaction time. The CS-TPP-MP nanocomposite was characterized by Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX), X-Ray Diffraction (XRD) and Fourier-Transform Infrared (FTIR) Spectroscopy. Catalepsy test was performed to find the antiparkinsonian activity level of CS-TPP-MP nanocomposite at doses of 5, 10, 15, 20, and 25 mg/kg body weight. Based on the results of CS-TPP-MP synthesis, it was found that the reactant composition (CS-TPP:MP) of 1:3 with reaction time of 20 minutes produced the highest yield (14.21%.) SEM-EDX characterization showed that the morphology of CS-TPP-MP nanocomposite was predominantly spherical and the size was approximately 120-170 nm with a composition of C = 55.43%, O = 30.46%, N = 13.46%, P = 0.44%. XRD diffractogram showed that CS-TPP-MP nanocomposite has amorphous structure. FTIR analysis showed the appearance of absorption at wavelength of 1643.35 cm−1 which proved the interaction between the primary amine group of chitosan and the carbonyl group of EMP. Catalepsy test demonstrated that CS-TPP-MP nanocomposite at the doses of 5, 10, 15, 20, and 25 mg/kg body weight could reduce catalepsy symptoms in mice significantly, and the best dosage was 20 mg/kg body weight. IOP Publishing 2020-05-01 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/28586/1/Synthesis%20and%20antiparkinsonian%20activity%20of%20nanocomposite%20of%20chitosan.pdf Sardjono, R. E. and Fauziyah, A. N. and Puspitasari, M. D. and Musthapa, I. and Khoerunnisa, F. and Azzahra, G. N. and R., Mamat and Erdiwansyah, . (2020) Synthesis and antiparkinsonian activity of nanocomposite of chitosan-tripolyphosphate-Mucuna pruriens L extract (CS-TPP-MP). In: IOP Conference Series: Materials Science and Engineering, 5th International Conference on Smart Materials Research (ICSMR 2019) , 13-16 November 2019 , Brisbane, Australia. pp. 1-7., 856 (012009). ISSN 1757-8981 (Print), 1757-899X (Online) (Published) https://doi.org/10.1088/1757-899X/856/1/012009 |
| spellingShingle | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry TP Chemical technology Sardjono, R. E. Fauziyah, A. N. Puspitasari, M. D. Musthapa, I. Khoerunnisa, F. Azzahra, G. N. R., Mamat Erdiwansyah, . Synthesis and antiparkinsonian activity of nanocomposite of chitosan-tripolyphosphate-Mucuna pruriens L extract (CS-TPP-MP) |
| title | Synthesis and antiparkinsonian activity of nanocomposite of chitosan-tripolyphosphate-Mucuna pruriens L extract (CS-TPP-MP) |
| title_full | Synthesis and antiparkinsonian activity of nanocomposite of chitosan-tripolyphosphate-Mucuna pruriens L extract (CS-TPP-MP) |
| title_fullStr | Synthesis and antiparkinsonian activity of nanocomposite of chitosan-tripolyphosphate-Mucuna pruriens L extract (CS-TPP-MP) |
| title_full_unstemmed | Synthesis and antiparkinsonian activity of nanocomposite of chitosan-tripolyphosphate-Mucuna pruriens L extract (CS-TPP-MP) |
| title_short | Synthesis and antiparkinsonian activity of nanocomposite of chitosan-tripolyphosphate-Mucuna pruriens L extract (CS-TPP-MP) |
| title_sort | synthesis and antiparkinsonian activity of nanocomposite of chitosan-tripolyphosphate-mucuna pruriens l extract (cs-tpp-mp) |
| topic | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/28586/ http://umpir.ump.edu.my/id/eprint/28586/ http://umpir.ump.edu.my/id/eprint/28586/1/Synthesis%20and%20antiparkinsonian%20activity%20of%20nanocomposite%20of%20chitosan.pdf |