Microwave-assisted extraction of andrographolide, 14-deoxy-11,12- didehydroandrographolide and neoandrographolide from andrographis paniculata: an optimisation study
Andrographis paniculata is often used as functional foods owing to its broad range of pharmaceutical activities due to the presence of main terpene compounds such as andrographolide, 14-deoxy-11,12-didehydroandrographolide and neoandrographolide. The aforementioned compounds must be extracted from t...
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| Language: | English English |
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Bentham Science
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/28505/ http://umpir.ump.edu.my/id/eprint/28505/1/Jolius%20Gimbun%20CNFS%20MS%202nd.pdf http://umpir.ump.edu.my/id/eprint/28505/7/Microwave-Assisted%20Extraction%20of%20Andrographolide.pdf |
| _version_ | 1848823070114971648 |
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| author | Chia, Vi Vien Pang, Sook Fun Abdullah, Sureena Yusoff, Mashitah Gimbun, Jolius |
| author_facet | Chia, Vi Vien Pang, Sook Fun Abdullah, Sureena Yusoff, Mashitah Gimbun, Jolius |
| author_sort | Chia, Vi Vien |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Andrographis paniculata is often used as functional foods owing to its broad range of pharmaceutical activities due to the presence of main terpene compounds such as andrographolide, 14-deoxy-11,12-didehydroandrographolide and neoandrographolide. The aforementioned compounds must be extracted from the plant material before it can be routinely used as a functional food ingredient. This work presents an optimisation study of microwave-assisted extraction (MAE) of terpene from Andrographis paniculata. In this study, two factors at three-level central composite face centred design was employed to optimise the process variables, including the effect of microwave power (75-175 W) and ethanol concentration (20-85%) on extraction of terpene from Andrographis paniculata. The experimental design was set based on initial screening on the effect of mesh size and solid solvent ratio and the present work was performed at fixed mesh size and solid solvent ratio, which corresponds to the highest yield established experimentally. The two most significant factors, i.e. microwave power and ethanol concentration was chosen for optimisation. Design Expert software generated 13 runs to optimise the yield of targeted terpene. Identification and quantification of the terpene were performed via Ultra-Performance Liquid Chromatography- Photodiode Array (UPLC-PDA). The optimum condition was found at microwave power of 140 W with 85% ethanol concentration, which produced the highest yield of andrographolide (10.926 ± 0.053 mg/g DW), 14-deoxy-11,12-didehydroandrographolide (4.336 ± 0.215 mg/g DW) and neoandrographolide (5.698 ± 0.252 mg/g DW). The model developed through response surface modelling has a desirability of 97.5% and the error between the experiment and the model predictions ranged from 2.4 to 5.2%. A successful optimisation of terpene extraction from A. paniculata via MAE was achieved at microwave power of 140 W and 85 % ethanol. The model developed from the regression analysis is sufficiently accurate and can be used to predict the yield of terpene extraction from A. paniculata. |
| first_indexed | 2025-11-15T02:51:17Z |
| format | Article |
| id | ump-28505 |
| institution | Universiti Malaysia Pahang |
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| language | English English |
| last_indexed | 2025-11-15T02:51:17Z |
| publishDate | 2020 |
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| spelling | ump-285052020-07-14T06:45:29Z http://umpir.ump.edu.my/id/eprint/28505/ Microwave-assisted extraction of andrographolide, 14-deoxy-11,12- didehydroandrographolide and neoandrographolide from andrographis paniculata: an optimisation study Chia, Vi Vien Pang, Sook Fun Abdullah, Sureena Yusoff, Mashitah Gimbun, Jolius TP Chemical technology Andrographis paniculata is often used as functional foods owing to its broad range of pharmaceutical activities due to the presence of main terpene compounds such as andrographolide, 14-deoxy-11,12-didehydroandrographolide and neoandrographolide. The aforementioned compounds must be extracted from the plant material before it can be routinely used as a functional food ingredient. This work presents an optimisation study of microwave-assisted extraction (MAE) of terpene from Andrographis paniculata. In this study, two factors at three-level central composite face centred design was employed to optimise the process variables, including the effect of microwave power (75-175 W) and ethanol concentration (20-85%) on extraction of terpene from Andrographis paniculata. The experimental design was set based on initial screening on the effect of mesh size and solid solvent ratio and the present work was performed at fixed mesh size and solid solvent ratio, which corresponds to the highest yield established experimentally. The two most significant factors, i.e. microwave power and ethanol concentration was chosen for optimisation. Design Expert software generated 13 runs to optimise the yield of targeted terpene. Identification and quantification of the terpene were performed via Ultra-Performance Liquid Chromatography- Photodiode Array (UPLC-PDA). The optimum condition was found at microwave power of 140 W with 85% ethanol concentration, which produced the highest yield of andrographolide (10.926 ± 0.053 mg/g DW), 14-deoxy-11,12-didehydroandrographolide (4.336 ± 0.215 mg/g DW) and neoandrographolide (5.698 ± 0.252 mg/g DW). The model developed through response surface modelling has a desirability of 97.5% and the error between the experiment and the model predictions ranged from 2.4 to 5.2%. A successful optimisation of terpene extraction from A. paniculata via MAE was achieved at microwave power of 140 W and 85 % ethanol. The model developed from the regression analysis is sufficiently accurate and can be used to predict the yield of terpene extraction from A. paniculata. Bentham Science 2020-02-28 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/28505/1/Jolius%20Gimbun%20CNFS%20MS%202nd.pdf pdf en http://umpir.ump.edu.my/id/eprint/28505/7/Microwave-Assisted%20Extraction%20of%20Andrographolide.pdf Chia, Vi Vien and Pang, Sook Fun and Abdullah, Sureena and Yusoff, Mashitah and Gimbun, Jolius (2020) Microwave-assisted extraction of andrographolide, 14-deoxy-11,12- didehydroandrographolide and neoandrographolide from andrographis paniculata: an optimisation study. Current Nutrition & Food Science, 16. pp. 1-8. ISSN 2212-3881. (Published) https://doi.org/10.2174/1573401316666200228120300 https://doi.org/10.2174/1573401316666200228120300 |
| spellingShingle | TP Chemical technology Chia, Vi Vien Pang, Sook Fun Abdullah, Sureena Yusoff, Mashitah Gimbun, Jolius Microwave-assisted extraction of andrographolide, 14-deoxy-11,12- didehydroandrographolide and neoandrographolide from andrographis paniculata: an optimisation study |
| title | Microwave-assisted extraction of andrographolide, 14-deoxy-11,12- didehydroandrographolide and neoandrographolide from andrographis paniculata: an optimisation study |
| title_full | Microwave-assisted extraction of andrographolide, 14-deoxy-11,12- didehydroandrographolide and neoandrographolide from andrographis paniculata: an optimisation study |
| title_fullStr | Microwave-assisted extraction of andrographolide, 14-deoxy-11,12- didehydroandrographolide and neoandrographolide from andrographis paniculata: an optimisation study |
| title_full_unstemmed | Microwave-assisted extraction of andrographolide, 14-deoxy-11,12- didehydroandrographolide and neoandrographolide from andrographis paniculata: an optimisation study |
| title_short | Microwave-assisted extraction of andrographolide, 14-deoxy-11,12- didehydroandrographolide and neoandrographolide from andrographis paniculata: an optimisation study |
| title_sort | microwave-assisted extraction of andrographolide, 14-deoxy-11,12- didehydroandrographolide and neoandrographolide from andrographis paniculata: an optimisation study |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/28505/ http://umpir.ump.edu.my/id/eprint/28505/ http://umpir.ump.edu.my/id/eprint/28505/ http://umpir.ump.edu.my/id/eprint/28505/1/Jolius%20Gimbun%20CNFS%20MS%202nd.pdf http://umpir.ump.edu.my/id/eprint/28505/7/Microwave-Assisted%20Extraction%20of%20Andrographolide.pdf |