Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation
Exploitation of soil bacteria for release of ferulic acid (FA) is extensively performed since bacteria are the largest soil community that have the potential in producing degrading enzymes. The current study aimed to isolate, identify and characterize the most efficient soil bacteria for high FA pro...
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Springer Nature
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/28134/ http://umpir.ump.edu.my/id/eprint/28134/2/23.1%20Isolation%2C%20identification%20and%20characterization%20of%20soil%20bacteria.pdf http://umpir.ump.edu.my/id/eprint/28134/13/Isolation%2C%20identifcation%20and%20characterization%20of%20soil%20bacteria.pdf |
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| author | Kamaliah, Abdul Samad Norazwina, Zainol Hafizuddin, Wan Yussof Zulsyazwan, Ahmad Khushairi Nurul Shareena Aqmar, Mohd Sharif Nur Syahirah, Mohd Shkuri |
| author_facet | Kamaliah, Abdul Samad Norazwina, Zainol Hafizuddin, Wan Yussof Zulsyazwan, Ahmad Khushairi Nurul Shareena Aqmar, Mohd Sharif Nur Syahirah, Mohd Shkuri |
| author_sort | Kamaliah, Abdul Samad |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Exploitation of soil bacteria for release of ferulic acid (FA) is extensively performed since bacteria are the largest soil community that have the potential in producing degrading enzymes. The current study aimed to isolate, identify and characterize the most efficient soil bacteria for high FA production from banana stem waste (BSW) via co-culture fermentation. Bacteria were isolated and screened from acclimatized mixture of soil culture and BSW. ARB programme package and biolog system were employed for identification and characterization. The results reveal that four isolates closely related to Bacillus spp. and one Lysinibacillus sp. had greater potential to produce FA in very large amounts. Specifically, the maximum FA yield of 394.76 mg/kg was achieved using co-culture of Bacillus sp. MB2, Bacillus sp. WB8A and B. pumilus strain WB1A, which was 2.5-fold higher than FA produced by single culture. Degradation activity was observed which suggests the possible utilization of substrate during the fermentation process. The results of the study demonstrate that exploitation of co-culture over single culture could be advantageous to facilitate improved FA production. |
| first_indexed | 2025-11-15T02:49:45Z |
| format | Article |
| id | ump-28134 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T02:49:45Z |
| publishDate | 2020 |
| publisher | Springer Nature |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-281342020-04-27T02:51:53Z http://umpir.ump.edu.my/id/eprint/28134/ Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation Kamaliah, Abdul Samad Norazwina, Zainol Hafizuddin, Wan Yussof Zulsyazwan, Ahmad Khushairi Nurul Shareena Aqmar, Mohd Sharif Nur Syahirah, Mohd Shkuri TP Chemical technology Exploitation of soil bacteria for release of ferulic acid (FA) is extensively performed since bacteria are the largest soil community that have the potential in producing degrading enzymes. The current study aimed to isolate, identify and characterize the most efficient soil bacteria for high FA production from banana stem waste (BSW) via co-culture fermentation. Bacteria were isolated and screened from acclimatized mixture of soil culture and BSW. ARB programme package and biolog system were employed for identification and characterization. The results reveal that four isolates closely related to Bacillus spp. and one Lysinibacillus sp. had greater potential to produce FA in very large amounts. Specifically, the maximum FA yield of 394.76 mg/kg was achieved using co-culture of Bacillus sp. MB2, Bacillus sp. WB8A and B. pumilus strain WB1A, which was 2.5-fold higher than FA produced by single culture. Degradation activity was observed which suggests the possible utilization of substrate during the fermentation process. The results of the study demonstrate that exploitation of co-culture over single culture could be advantageous to facilitate improved FA production. Springer Nature 2020 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/28134/2/23.1%20Isolation%2C%20identification%20and%20characterization%20of%20soil%20bacteria.pdf pdf en http://umpir.ump.edu.my/id/eprint/28134/13/Isolation%2C%20identifcation%20and%20characterization%20of%20soil%20bacteria.pdf Kamaliah, Abdul Samad and Norazwina, Zainol and Hafizuddin, Wan Yussof and Zulsyazwan, Ahmad Khushairi and Nurul Shareena Aqmar, Mohd Sharif and Nur Syahirah, Mohd Shkuri (2020) Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation. SN Applied Sciences, 2 (339). pp. 1-12. ISSN 2523-3963 (Print); 2523-3971 (Online). (Published) https://doi.org/10.1007/s42452-020-2151-3 https://doi.org/10.1007/s42452-020-2151-3 |
| spellingShingle | TP Chemical technology Kamaliah, Abdul Samad Norazwina, Zainol Hafizuddin, Wan Yussof Zulsyazwan, Ahmad Khushairi Nurul Shareena Aqmar, Mohd Sharif Nur Syahirah, Mohd Shkuri Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation |
| title | Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation |
| title_full | Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation |
| title_fullStr | Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation |
| title_full_unstemmed | Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation |
| title_short | Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation |
| title_sort | isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/28134/ http://umpir.ump.edu.my/id/eprint/28134/ http://umpir.ump.edu.my/id/eprint/28134/ http://umpir.ump.edu.my/id/eprint/28134/2/23.1%20Isolation%2C%20identification%20and%20characterization%20of%20soil%20bacteria.pdf http://umpir.ump.edu.my/id/eprint/28134/13/Isolation%2C%20identifcation%20and%20characterization%20of%20soil%20bacteria.pdf |