Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production
E. coli has been engineered to produce xylitol, but the production faces bottlenecks in terms of production yield and cell viability. In this study, recombinant E. coli (rE. coli) was immobilized on untreated and treated multiwalled carbon nanotubes (MWCNTs) for xylitol production. The immobilized r...
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Elsevier
2020
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| Online Access: | http://umpir.ump.edu.my/id/eprint/27774/ http://umpir.ump.edu.my/id/eprint/27774/1/Immobilization%20of%20recombinant%20Escherichia%20coli%20on%20multi-walled%20.pdf |
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| author | Noor Hidayah, Abd Rahman Jamaliah, Md. Jahim Mimi Sakinah, A. M. Roshanida, A. Rahman Siti Fatimah Zaharah, Fuzi Rosli Md, Illias |
| author_facet | Noor Hidayah, Abd Rahman Jamaliah, Md. Jahim Mimi Sakinah, A. M. Roshanida, A. Rahman Siti Fatimah Zaharah, Fuzi Rosli Md, Illias |
| author_sort | Noor Hidayah, Abd Rahman |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | E. coli has been engineered to produce xylitol, but the production faces bottlenecks in terms of production yield and cell viability. In this study, recombinant E. coli (rE. coli) was immobilized on untreated and treated multiwalled carbon nanotubes (MWCNTs) for xylitol production. The immobilized rE. coli on untreated MWCNTs gave the highest xylitol production (5.47 g L−1) and a productivity of 0.22 g L−1 h−1. The doubling time for the immobilized cells increased up to 20.40 h and was higher than that of free cells (3.67 h). Cell lysis of the immobilized cells was reduced by up to 73 %, and plasmid stability improved by up to 17 % compared to those of free cells. Xylitol production using the optimum parameters (pH 7.4, 0.005 mM and 29 °C) achieved a xylitol production and productivity of 6.33 g L−1 and 0.26 g L−1 h−1, respectively. A seven-cycle repeated batch fermentation was carried out for up to 168 h, which showed maximum xylitol production of 7.36 g L−1 during the third cycle. Hence, this new adsorption immobilization system using MWCNTs is an alternative to improve the production of xylitol. |
| first_indexed | 2025-11-15T02:48:16Z |
| format | Article |
| id | ump-27774 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:48:16Z |
| publishDate | 2020 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-277742021-01-15T08:03:42Z http://umpir.ump.edu.my/id/eprint/27774/ Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production Noor Hidayah, Abd Rahman Jamaliah, Md. Jahim Mimi Sakinah, A. M. Roshanida, A. Rahman Siti Fatimah Zaharah, Fuzi Rosli Md, Illias TP Chemical technology E. coli has been engineered to produce xylitol, but the production faces bottlenecks in terms of production yield and cell viability. In this study, recombinant E. coli (rE. coli) was immobilized on untreated and treated multiwalled carbon nanotubes (MWCNTs) for xylitol production. The immobilized rE. coli on untreated MWCNTs gave the highest xylitol production (5.47 g L−1) and a productivity of 0.22 g L−1 h−1. The doubling time for the immobilized cells increased up to 20.40 h and was higher than that of free cells (3.67 h). Cell lysis of the immobilized cells was reduced by up to 73 %, and plasmid stability improved by up to 17 % compared to those of free cells. Xylitol production using the optimum parameters (pH 7.4, 0.005 mM and 29 °C) achieved a xylitol production and productivity of 6.33 g L−1 and 0.26 g L−1 h−1, respectively. A seven-cycle repeated batch fermentation was carried out for up to 168 h, which showed maximum xylitol production of 7.36 g L−1 during the third cycle. Hence, this new adsorption immobilization system using MWCNTs is an alternative to improve the production of xylitol. Elsevier 2020-04 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/27774/1/Immobilization%20of%20recombinant%20Escherichia%20coli%20on%20multi-walled%20.pdf Noor Hidayah, Abd Rahman and Jamaliah, Md. Jahim and Mimi Sakinah, A. M. and Roshanida, A. Rahman and Siti Fatimah Zaharah, Fuzi and Rosli Md, Illias (2020) Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production. Enzyme and Microbial Technology, 135 (109495). pp. 1-10. ISSN 0141-0229. (Published) https://doi.org/10.1016/j.enzmictec.2019.109495 https://doi.org/10.1016/j.enzmictec.2019.109495 |
| spellingShingle | TP Chemical technology Noor Hidayah, Abd Rahman Jamaliah, Md. Jahim Mimi Sakinah, A. M. Roshanida, A. Rahman Siti Fatimah Zaharah, Fuzi Rosli Md, Illias Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production |
| title | Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production |
| title_full | Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production |
| title_fullStr | Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production |
| title_full_unstemmed | Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production |
| title_short | Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production |
| title_sort | immobilization of recombinant escherichia coli on multi-walled carbon nanotubes for xylitol production |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/27774/ http://umpir.ump.edu.my/id/eprint/27774/ http://umpir.ump.edu.my/id/eprint/27774/ http://umpir.ump.edu.my/id/eprint/27774/1/Immobilization%20of%20recombinant%20Escherichia%20coli%20on%20multi-walled%20.pdf |