Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia
The present study aims to detect CTX-M-type ESBL from Escherichia coli clinical isolates and to analyze their antibotic susceptibility patterns. One hundred of E. coli isolates were collected from different clinical samples from a tertiary hospital. ESBL positivity was determined by the disk diffusi...
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| Format: | Article |
| Language: | English |
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University of Baghdad
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/27552/ http://umpir.ump.edu.my/id/eprint/27552/1/Detection%20of%20CTX-M-type%20ESBLs%20from%20escherichia%20coli.pdf |
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| author | M. K. K., Fazlul Y., Farzana A., Najnin M. A., Rashid M. H. M., Nazmul |
| author_facet | M. K. K., Fazlul Y., Farzana A., Najnin M. A., Rashid M. H. M., Nazmul |
| author_sort | M. K. K., Fazlul |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The present study aims to detect CTX-M-type ESBL from Escherichia coli clinical isolates and to analyze their antibotic susceptibility patterns. One hundred of E. coli isolates were collected from different clinical samples from a tertiary hospital. ESBL positivity was determined by the disk diffusion method. PCR used for amplification of CTX-M-type ESBL produced by E. coli. Out of 100 E. coli isolates, twenty-four isolates (24%) were ESBL-producers. E. coli isolated from pus was the most frequent clinical specimen that produced ESBL (41.66%) followed by urine (34.21%), respiratory (22.23%), and blood (19.05%). After PCR amplification of these 24 isolates, 10 (41.66%) isolates were found to possess CTX-M genes. The CTX-M type ESBL producing E. coli against antibiotics belonging to different families showed the highest resistance rates to Ampicillin (100%), Cefotaxime (97%), Cefuroxime (95%), and Ciprofoxacin (86%). Carbapenem groups of antibiotics, Meropenem (89%) and Imipenem (85%) have the highest susceptibility rate among all antibiotics used in this study. The outcome of the antimicrobial susceptibility testing of significant CTX-M- type ESBL producing E. coli could be useful to avoid failure or prolong treatments. |
| first_indexed | 2025-11-15T02:47:25Z |
| format | Article |
| id | ump-27552 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:47:25Z |
| publishDate | 2019 |
| publisher | University of Baghdad |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-275522020-03-31T23:53:49Z http://umpir.ump.edu.my/id/eprint/27552/ Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia M. K. K., Fazlul Y., Farzana A., Najnin M. A., Rashid M. H. M., Nazmul QD Chemistry QP Physiology RA Public aspects of medicine TP Chemical technology The present study aims to detect CTX-M-type ESBL from Escherichia coli clinical isolates and to analyze their antibotic susceptibility patterns. One hundred of E. coli isolates were collected from different clinical samples from a tertiary hospital. ESBL positivity was determined by the disk diffusion method. PCR used for amplification of CTX-M-type ESBL produced by E. coli. Out of 100 E. coli isolates, twenty-four isolates (24%) were ESBL-producers. E. coli isolated from pus was the most frequent clinical specimen that produced ESBL (41.66%) followed by urine (34.21%), respiratory (22.23%), and blood (19.05%). After PCR amplification of these 24 isolates, 10 (41.66%) isolates were found to possess CTX-M genes. The CTX-M type ESBL producing E. coli against antibiotics belonging to different families showed the highest resistance rates to Ampicillin (100%), Cefotaxime (97%), Cefuroxime (95%), and Ciprofoxacin (86%). Carbapenem groups of antibiotics, Meropenem (89%) and Imipenem (85%) have the highest susceptibility rate among all antibiotics used in this study. The outcome of the antimicrobial susceptibility testing of significant CTX-M- type ESBL producing E. coli could be useful to avoid failure or prolong treatments. University of Baghdad 2019-09-22 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/27552/1/Detection%20of%20CTX-M-type%20ESBLs%20from%20escherichia%20coli.pdf M. K. K., Fazlul and Y., Farzana and A., Najnin and M. A., Rashid and M. H. M., Nazmul (2019) Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia. Baghdad Science Journal, 16 (3). pp. 682-688. ISSN 2078-8665. (Published) https://doi.org/10.21123/bsj.2019.16.3(Suppl.).0682 https://doi.org/10.21123/bsj.2019.16.3(Suppl.).0682 |
| spellingShingle | QD Chemistry QP Physiology RA Public aspects of medicine TP Chemical technology M. K. K., Fazlul Y., Farzana A., Najnin M. A., Rashid M. H. M., Nazmul Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia |
| title | Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia |
| title_full | Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia |
| title_fullStr | Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia |
| title_full_unstemmed | Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia |
| title_short | Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a Tertiary Hospital, Malaysia |
| title_sort | detection of ctx-m-type esbls from escherichia coli clinical isolates from a tertiary hospital, malaysia |
| topic | QD Chemistry QP Physiology RA Public aspects of medicine TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/27552/ http://umpir.ump.edu.my/id/eprint/27552/ http://umpir.ump.edu.my/id/eprint/27552/ http://umpir.ump.edu.my/id/eprint/27552/1/Detection%20of%20CTX-M-type%20ESBLs%20from%20escherichia%20coli.pdf |