Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase
Biocatalysis is a powerful tool for organic synthesis, especially for the synthesis of high value products such as chiral molecules and intermediates. Chiral amines can be synthesized by kinetic resolution of racemic amines or by the asymmetric synthesis from prochiral ketones. Kinetic parameter est...
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| Format: | Conference or Workshop Item |
| Language: | English English |
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2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/26906/ http://umpir.ump.edu.my/id/eprint/26906/1/88.%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf http://umpir.ump.edu.my/id/eprint/26906/2/88.1%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf |
| _version_ | 1848822654163746816 |
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| author | S. D., Vijaya Kumar Zainatul Bahiyah, Handani Rohana, Abu |
| author_facet | S. D., Vijaya Kumar Zainatul Bahiyah, Handani Rohana, Abu |
| author_sort | S. D., Vijaya Kumar |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Biocatalysis is a powerful tool for organic synthesis, especially for the synthesis of high value products such as chiral molecules and intermediates. Chiral amines can be synthesized by kinetic resolution of racemic amines or by the asymmetric synthesis from prochiral ketones. Kinetic parameter estimation of such biocatalytic reaction is useful to evaluate process and technology options. In this research, the Michaelis–Menten kinetic parameters from the asymmetric synthesis of (R)-1-phenylethylamine from acetophenone and alanine with immobilized ω-transaminase were estimated. The immobilized ω-transaminase was prepared by entrapment with diaion beads. The kinetic parameters such as, Michaelis-Menten constant (Km) and maximum rate of reaction (Vmax) were measured using initial rate experiments by varying the substrate acetophenone concentrations (2 mM to 10 mM) at 100 mM of amino donor. The kinetic parameters were then estimated by Lineweaver-burk analysis. The Vmax and Km was estimated at 6.33 mM/min and 0.382 mM, respectively. |
| first_indexed | 2025-11-15T02:44:40Z |
| format | Conference or Workshop Item |
| id | ump-26906 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T02:44:40Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-269062020-03-18T02:29:02Z http://umpir.ump.edu.my/id/eprint/26906/ Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase S. D., Vijaya Kumar Zainatul Bahiyah, Handani Rohana, Abu TP Chemical technology Biocatalysis is a powerful tool for organic synthesis, especially for the synthesis of high value products such as chiral molecules and intermediates. Chiral amines can be synthesized by kinetic resolution of racemic amines or by the asymmetric synthesis from prochiral ketones. Kinetic parameter estimation of such biocatalytic reaction is useful to evaluate process and technology options. In this research, the Michaelis–Menten kinetic parameters from the asymmetric synthesis of (R)-1-phenylethylamine from acetophenone and alanine with immobilized ω-transaminase were estimated. The immobilized ω-transaminase was prepared by entrapment with diaion beads. The kinetic parameters such as, Michaelis-Menten constant (Km) and maximum rate of reaction (Vmax) were measured using initial rate experiments by varying the substrate acetophenone concentrations (2 mM to 10 mM) at 100 mM of amino donor. The kinetic parameters were then estimated by Lineweaver-burk analysis. The Vmax and Km was estimated at 6.33 mM/min and 0.382 mM, respectively. 2019 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/26906/1/88.%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf pdf en http://umpir.ump.edu.my/id/eprint/26906/2/88.1%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf S. D., Vijaya Kumar and Zainatul Bahiyah, Handani and Rohana, Abu (2019) Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase. In: Energy Security and Chemical Engineering Congress (ESCHE2019) , 17-19 July 2019 , Parkroyal Resort Penang, Malaysia. pp. 1-4.. (Unpublished) (Unpublished) |
| spellingShingle | TP Chemical technology S. D., Vijaya Kumar Zainatul Bahiyah, Handani Rohana, Abu Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase |
| title | Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase |
| title_full | Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase |
| title_fullStr | Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase |
| title_full_unstemmed | Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase |
| title_short | Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase |
| title_sort | kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/26906/ http://umpir.ump.edu.my/id/eprint/26906/1/88.%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf http://umpir.ump.edu.my/id/eprint/26906/2/88.1%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf |