Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing
Herein, we propose a new approach to fabricate an enzyme electrode based on immobilization of glucose oxidase (GOD) with multiporous nanofibers (MPNFs) of SnO2 on Prussian blue (PB) modified gold (Au) electrode with chitosan. MPNFs of SnO2 were synthesized by electrospinning method from the tin. Pru...
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| Format: | Article |
| Language: | English |
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Elsevier
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/26719/ http://umpir.ump.edu.my/id/eprint/26719/1/Fabrication%20of%20a%20glucose%20oxidase-multiporous%20tin-oxide%20.pdf |
| _version_ | 1848822605326319616 |
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| author | A. K. M., Kafi Alim, Samiul Rajan, Jose M. M., Yusoff |
| author_facet | A. K. M., Kafi Alim, Samiul Rajan, Jose M. M., Yusoff |
| author_sort | A. K. M., Kafi |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Herein, we propose a new approach to fabricate an enzyme electrode based on immobilization of glucose oxidase (GOD) with multiporous nanofibers (MPNFs) of SnO2 on Prussian blue (PB) modified gold (Au) electrode with chitosan. MPNFs of SnO2 were synthesized by electrospinning method from the tin. Prussian blue was electrochemically deposited onto an Au electrode. GOD was then co-immobilized with the nanofibers on the surface of the PB modified gold electrode by using chitosan. The immobilized GOD/MPNFs onto the PB-Au presented faster, more stable and sensitive amperometric response to detecting glucose. Hydrogen peroxide (H2O2) produced via Glucose oxidation and glucose reaction was detected by Prussian blue. The linear response dependence on glucose concentration was observed in a range of 0.5–5 mM and a detection limit of 0.05 mM at 3 signal/noise ratio. Because of unique structural characteristics at the nanoscale level, multiporous nanofibers (MPNFs) of SnO2 is an ideal candidate for matrices in biosensing. |
| first_indexed | 2025-11-15T02:43:54Z |
| format | Article |
| id | ump-26719 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:43:54Z |
| publishDate | 2019 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-267192020-03-05T09:00:31Z http://umpir.ump.edu.my/id/eprint/26719/ Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing A. K. M., Kafi Alim, Samiul Rajan, Jose M. M., Yusoff QD Chemistry TP Chemical technology Herein, we propose a new approach to fabricate an enzyme electrode based on immobilization of glucose oxidase (GOD) with multiporous nanofibers (MPNFs) of SnO2 on Prussian blue (PB) modified gold (Au) electrode with chitosan. MPNFs of SnO2 were synthesized by electrospinning method from the tin. Prussian blue was electrochemically deposited onto an Au electrode. GOD was then co-immobilized with the nanofibers on the surface of the PB modified gold electrode by using chitosan. The immobilized GOD/MPNFs onto the PB-Au presented faster, more stable and sensitive amperometric response to detecting glucose. Hydrogen peroxide (H2O2) produced via Glucose oxidation and glucose reaction was detected by Prussian blue. The linear response dependence on glucose concentration was observed in a range of 0.5–5 mM and a detection limit of 0.05 mM at 3 signal/noise ratio. Because of unique structural characteristics at the nanoscale level, multiporous nanofibers (MPNFs) of SnO2 is an ideal candidate for matrices in biosensing. Elsevier 2019-10-07 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/26719/1/Fabrication%20of%20a%20glucose%20oxidase-multiporous%20tin-oxide%20.pdf A. K. M., Kafi and Alim, Samiul and Rajan, Jose and M. M., Yusoff (2019) Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing. Journal of Electroanalytical Chemistry, 852 (113550). pp. 1-6. ISSN 1572-6657. (Published) https://doi.org/10.1016/j.jelechem.2019.113550 https://doi.org/10.1016/j.jelechem.2019.113550 |
| spellingShingle | QD Chemistry TP Chemical technology A. K. M., Kafi Alim, Samiul Rajan, Jose M. M., Yusoff Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing |
| title | Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing |
| title_full | Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing |
| title_fullStr | Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing |
| title_full_unstemmed | Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing |
| title_short | Fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on Prussian blue–modified gold electrode for biosensing |
| title_sort | fabrication of a glucose oxidase/multiporous tin-oxide nanofiber film on prussian blue–modified gold electrode for biosensing |
| topic | QD Chemistry TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/26719/ http://umpir.ump.edu.my/id/eprint/26719/ http://umpir.ump.edu.my/id/eprint/26719/ http://umpir.ump.edu.my/id/eprint/26719/1/Fabrication%20of%20a%20glucose%20oxidase-multiporous%20tin-oxide%20.pdf |