Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3)
Xylonic acid is one of the top 30 highly valuable chemicals that can be obtained by microbial conversion. One of the recently used microorganisms to produce xylonic acid from xylose is the recombinant Escherichia coli. In this study, the effect of medium and nitrogen source concentration on the grow...
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| Format: | Article |
| Language: | English |
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Elsevier Ltd
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/25948/ http://umpir.ump.edu.my/id/eprint/25948/1/Effects%20of%20culture%20conditions%20on%20growth%20and%20production1.pdf |
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| author | Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah Nurul Atikah, Mohd Rodzri Mior Ahmad Khushairi, Mohd Zahari Wan Salwanis, Wan Md. Zain |
| author_facet | Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah Nurul Atikah, Mohd Rodzri Mior Ahmad Khushairi, Mohd Zahari Wan Salwanis, Wan Md. Zain |
| author_sort | Rafidah, Mohamed Ali Hanapiah |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Xylonic acid is one of the top 30 highly valuable chemicals that can be obtained by microbial conversion. One of the recently used microorganisms to produce xylonic acid from xylose is the recombinant Escherichia coli. In this study, the effect of medium and nitrogen source concentration on the growth of the recombinant E. coli BL21 (DE3) and xylonic acid production were investigated. The fermentation was carried out in shake flasks for 24 h at 37 °C and 200 rpm. Hydroxamate analysis was performed to determine the concentration of xylonic acid. The recombinant E. coli BL21 (DE3) produced up to 8.69 g/L xylonic acid with yield of 0.86 g/g from 10 g/L xylose in SOB medium with 20 g/L tryptone and 5 g/L yeast extract. |
| first_indexed | 2025-11-15T02:40:47Z |
| format | Article |
| id | ump-25948 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:40:47Z |
| publishDate | 2019 |
| publisher | Elsevier Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-259482021-02-09T02:34:59Z http://umpir.ump.edu.my/id/eprint/25948/ Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3) Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah Nurul Atikah, Mohd Rodzri Mior Ahmad Khushairi, Mohd Zahari Wan Salwanis, Wan Md. Zain TP Chemical technology Xylonic acid is one of the top 30 highly valuable chemicals that can be obtained by microbial conversion. One of the recently used microorganisms to produce xylonic acid from xylose is the recombinant Escherichia coli. In this study, the effect of medium and nitrogen source concentration on the growth of the recombinant E. coli BL21 (DE3) and xylonic acid production were investigated. The fermentation was carried out in shake flasks for 24 h at 37 °C and 200 rpm. Hydroxamate analysis was performed to determine the concentration of xylonic acid. The recombinant E. coli BL21 (DE3) produced up to 8.69 g/L xylonic acid with yield of 0.86 g/g from 10 g/L xylose in SOB medium with 20 g/L tryptone and 5 g/L yeast extract. Elsevier Ltd 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25948/1/Effects%20of%20culture%20conditions%20on%20growth%20and%20production1.pdf Rafidah, Mohamed Ali Hanapiah and Rozaimi, Abu Samah and Nurul Atikah, Mohd Rodzri and Mior Ahmad Khushairi, Mohd Zahari and Wan Salwanis, Wan Md. Zain (2019) Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3). Materials Today: Proceedings, 19 (Part 4). pp. 1467-1472. ISSN 2214-7853. (Published) https://doi.org/10.1016/j.matpr.2019.11.170 https://doi.org/10.1016/j.matpr.2019.11.170 |
| spellingShingle | TP Chemical technology Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah Nurul Atikah, Mohd Rodzri Mior Ahmad Khushairi, Mohd Zahari Wan Salwanis, Wan Md. Zain Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3) |
| title | Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3) |
| title_full | Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3) |
| title_fullStr | Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3) |
| title_full_unstemmed | Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3) |
| title_short | Effects of Culture Conditions on Growth and Production of Xylonic Acid using Recombinant E. coli BL21 (DE3) |
| title_sort | effects of culture conditions on growth and production of xylonic acid using recombinant e. coli bl21 (de3) |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/25948/ http://umpir.ump.edu.my/id/eprint/25948/ http://umpir.ump.edu.my/id/eprint/25948/ http://umpir.ump.edu.my/id/eprint/25948/1/Effects%20of%20culture%20conditions%20on%20growth%20and%20production1.pdf |