A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study
The telmisartan was determined in a rat plasma using developed and validated a reversed-phase high performance liquid chromatographic (HPLC). The pre-treatment of the plasma sample involving liquid-liquid extraction using ethanol as the extracting solvent. The HPLC method validation has been shown a...
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A and V publication
2021
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| Online Access: | http://umpir.ump.edu.my/id/eprint/25861/ http://umpir.ump.edu.my/id/eprint/25861/1/A%20Bioanalytical%20Method%20for%20Quantification%20of%20Telmisartan%20in%20rat%20Plasma%20Development%20Validation%20and%20Application%20to%20Pharmacokinetic%20Study.pdf |
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| author | AL-japairai, Khater Ahmed Saeed Chatterjee, Bappaditya Mahmood, Syed Almurisi, Samah Hamed |
| author_facet | AL-japairai, Khater Ahmed Saeed Chatterjee, Bappaditya Mahmood, Syed Almurisi, Samah Hamed |
| author_sort | AL-japairai, Khater Ahmed Saeed |
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| collection | Online Access |
| description | The telmisartan was determined in a rat plasma using developed and validated a reversed-phase high performance liquid chromatographic (HPLC). The pre-treatment of the plasma sample involving liquid-liquid extraction using ethanol as the extracting solvent. The HPLC method validation has been shown a linear calibration curve over a plasma concentrations range of 0.7 to 10µg/mL with a correlation coefficient of 0.9979, the limit of detection and the limit of quantification were determined to be 0.025µg/ml and 0.07µg/ml, respectively. The precision and accuracy were in an acceptable limit. The pharmacokinetic parameters of telmisartan were adequately evaluated following a single oral dose (4mg/kg) in Sprague-Dawley rats. The results observed conclude that the developed bioanalytical HPLC method is appropriate and applicable as an analytical tool in the pharmacokinetic study of telmisartan. |
| first_indexed | 2025-11-15T02:40:25Z |
| format | Article |
| id | ump-25861 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:40:25Z |
| publishDate | 2021 |
| publisher | A and V publication |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-258612021-07-15T09:15:23Z http://umpir.ump.edu.my/id/eprint/25861/ A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study AL-japairai, Khater Ahmed Saeed Chatterjee, Bappaditya Mahmood, Syed Almurisi, Samah Hamed Q Science (General) The telmisartan was determined in a rat plasma using developed and validated a reversed-phase high performance liquid chromatographic (HPLC). The pre-treatment of the plasma sample involving liquid-liquid extraction using ethanol as the extracting solvent. The HPLC method validation has been shown a linear calibration curve over a plasma concentrations range of 0.7 to 10µg/mL with a correlation coefficient of 0.9979, the limit of detection and the limit of quantification were determined to be 0.025µg/ml and 0.07µg/ml, respectively. The precision and accuracy were in an acceptable limit. The pharmacokinetic parameters of telmisartan were adequately evaluated following a single oral dose (4mg/kg) in Sprague-Dawley rats. The results observed conclude that the developed bioanalytical HPLC method is appropriate and applicable as an analytical tool in the pharmacokinetic study of telmisartan. A and V publication 2021 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25861/1/A%20Bioanalytical%20Method%20for%20Quantification%20of%20Telmisartan%20in%20rat%20Plasma%20Development%20Validation%20and%20Application%20to%20Pharmacokinetic%20Study.pdf AL-japairai, Khater Ahmed Saeed and Chatterjee, Bappaditya and Mahmood, Syed and Almurisi, Samah Hamed (2021) A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study. Research J. Pharm. and Tech, 14 (4). pp. 2139-2144. ISSN 0974-360X. (Published) https: //doi.org/10.52711/0974-360X.2021.00379 |
| spellingShingle | Q Science (General) AL-japairai, Khater Ahmed Saeed Chatterjee, Bappaditya Mahmood, Syed Almurisi, Samah Hamed A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study |
| title | A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study |
| title_full | A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study |
| title_fullStr | A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study |
| title_full_unstemmed | A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study |
| title_short | A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study |
| title_sort | bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study |
| topic | Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/25861/ http://umpir.ump.edu.my/id/eprint/25861/ http://umpir.ump.edu.my/id/eprint/25861/1/A%20Bioanalytical%20Method%20for%20Quantification%20of%20Telmisartan%20in%20rat%20Plasma%20Development%20Validation%20and%20Application%20to%20Pharmacokinetic%20Study.pdf |