Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-cente...
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| Format: | Conference or Workshop Item |
| Language: | English English |
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2018
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| Online Access: | http://umpir.ump.edu.my/id/eprint/25502/ http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf |
| _version_ | 1848822295945019392 |
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| author | Zatul Iffah, Mohd Arshad Azura, Amid |
| author_facet | Zatul Iffah, Mohd Arshad Azura, Amid |
| author_sort | Zatul Iffah, Mohd Arshad |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel. |
| first_indexed | 2025-11-15T02:38:59Z |
| format | Conference or Workshop Item |
| id | ump-25502 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T02:38:59Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-255022019-11-21T03:24:52Z http://umpir.ump.edu.my/id/eprint/25502/ Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology Zatul Iffah, Mohd Arshad Azura, Amid TP Chemical technology Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel. 2018 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf pdf en http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf Zatul Iffah, Mohd Arshad and Azura, Amid (2018) Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology. In: The 4th International Conference On The Applications Of Science And Mathematics 2018 , 13 - 14 Ogos 2018 , Tunku Tun Aminah Library UTHM, Parit Raja, Johor. pp. 377-382., 7 (4.30). (Published) http://dx.doi.org/10.14419/ijet.v7i4.30.22326 |
| spellingShingle | TP Chemical technology Zatul Iffah, Mohd Arshad Azura, Amid Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology |
| title | Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology |
| title_full | Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology |
| title_fullStr | Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology |
| title_full_unstemmed | Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology |
| title_short | Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology |
| title_sort | optimization of aqueous two-phase system (atps) of recombinant bromelain by response surface methodology |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/25502/ http://umpir.ump.edu.my/id/eprint/25502/ http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf |