Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T)
Factors influencing poly(3-hydroxybutyrate) P(3HB) production by Cupriavidus necator C C U G 5 2 2 3 8T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were in...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2012
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| Online Access: | http://umpir.ump.edu.my/id/eprint/25286/ http://umpir.ump.edu.my/id/eprint/25286/1/Factors%20affecting%20poly%283-hydroxybutyrate%29%20production%20from%20oil%20palm.pdf |
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| author | Mior Ahmad Khushairi, Mohd Zahari Hidayah, Ariffin Mohd Noriznan, Mokhtar Jailani, Salihon Shirai, Yoshihito Mohd Ali, Hassan |
| author_facet | Mior Ahmad Khushairi, Mohd Zahari Hidayah, Ariffin Mohd Noriznan, Mokhtar Jailani, Salihon Shirai, Yoshihito Mohd Ali, Hassan |
| author_sort | Mior Ahmad Khushairi, Mohd Zahari |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Factors influencing poly(3-hydroxybutyrate) P(3HB) production by Cupriavidus necator C C U G 5 2 2 3 8T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH4)2SO4 concentration at 0.5 g/L. By culturing the wild-type strain of C. necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ±0.13 g/L and 45 ±1.5 wt.%, respectively. This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition. In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor. Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%. P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate. |
| first_indexed | 2025-11-15T02:38:09Z |
| format | Article |
| id | ump-25286 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:38:09Z |
| publishDate | 2012 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-252862019-07-18T02:22:55Z http://umpir.ump.edu.my/id/eprint/25286/ Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T) Mior Ahmad Khushairi, Mohd Zahari Hidayah, Ariffin Mohd Noriznan, Mokhtar Jailani, Salihon Shirai, Yoshihito Mohd Ali, Hassan TP Chemical technology Factors influencing poly(3-hydroxybutyrate) P(3HB) production by Cupriavidus necator C C U G 5 2 2 3 8T utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH4)2SO4 concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB) content was recorded at pH 7.0, agitation speed of 220 rpm, and (NH4)2SO4 concentration at 0.5 g/L. By culturing the wild-type strain of C. necator under the aforementioned conditions, the cell dry weight (CDW) and P(3HB) content obtained were 9.31 ±0.13 g/L and 45 ±1.5 wt.%, respectively. This accounted for 40% increment of P(3HB) content compared to the nonoptimized condition. In the meanwhile, the effect of dissolved oxygen tension (DOT) on P(3HB) production was investigated in a 2-L bioreactor. Highest CDW (11.37 g/L) and P(3HB) content (44 wt.%) were achieved when DOT level was set at 30%. P(3HB) produced from OPF juice had a tensile strength of 40 MPa and elongation at break of 8% demonstrated that P(3HB) produced from renewable and cheap carbon source is comparable to those produced from commercial substrate. Hindawi Publishing Corporation 2012 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25286/1/Factors%20affecting%20poly%283-hydroxybutyrate%29%20production%20from%20oil%20palm.pdf Mior Ahmad Khushairi, Mohd Zahari and Hidayah, Ariffin and Mohd Noriznan, Mokhtar and Jailani, Salihon and Shirai, Yoshihito and Mohd Ali, Hassan (2012) Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T). Journal of Biomedicine and Biotechnology, 2012 (125865). pp. 1-8. ISSN 1110-7243. (Published) https://doi.org/10.1155/2012/125865 https://doi.org/10.1155/2012/125865 |
| spellingShingle | TP Chemical technology Mior Ahmad Khushairi, Mohd Zahari Hidayah, Ariffin Mohd Noriznan, Mokhtar Jailani, Salihon Shirai, Yoshihito Mohd Ali, Hassan Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T) |
| title | Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T) |
| title_full | Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T) |
| title_fullStr | Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T) |
| title_full_unstemmed | Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T) |
| title_short | Factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (CCUG52238 T) |
| title_sort | factors affecting poly(3-hydroxybutyrate) production from oil palm frond juice by cupriavidus necator (ccug52238 t) |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/25286/ http://umpir.ump.edu.my/id/eprint/25286/ http://umpir.ump.edu.my/id/eprint/25286/ http://umpir.ump.edu.my/id/eprint/25286/1/Factors%20affecting%20poly%283-hydroxybutyrate%29%20production%20from%20oil%20palm.pdf |