Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay
The measurement of biological targets using nanoparticle magnetic markers has been extensively studied for its applications in magnetic immunoassays (MIAs). Compared with the optical immunoassay method, the MIA methods have some attractive characteristics such as a wash-free process that does not ne...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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IEEE
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/25077/ http://umpir.ump.edu.my/id/eprint/25077/1/Using%20Magnetic%20Field%20Gradients%20to%20Shorten%20the%20Antigen1.pdf |
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| author | Tsukada, Keiji Tsunashima, Kenta Jinno, Katsuya Hiramatsu, Bunta Takeuchi, Shun Fujimoto, Kayo Kenji, Sakai Kiwa, Toshihiko Mohd Mawardi, Saari |
| author_facet | Tsukada, Keiji Tsunashima, Kenta Jinno, Katsuya Hiramatsu, Bunta Takeuchi, Shun Fujimoto, Kayo Kenji, Sakai Kiwa, Toshihiko Mohd Mawardi, Saari |
| author_sort | Tsukada, Keiji |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The measurement of biological targets using nanoparticle magnetic markers has been extensively studied for its applications in magnetic immunoassays (MIAs). Compared with the optical immunoassay method, the MIA methods have some attractive characteristics such as a wash-free process that does not need bound/free marker separation and the ability to detect biological targets in a nebulous liquid. In addition, the wash-free process is very fast. In this paper, we tried to reduce the reaction time for the antigen-antibody reaction in order to make the MIA method more attractive. The reduction in reaction time and the detection characteristic of biological targets were demonstrated using C-reactive proteins (CRPs) as biological targets for diagnosing inflammation. To shorten the reaction time, a magnetic-shaking method using the magnetic field gradient from a neodymium magnet was developed. When comparing the reaction times with and without magnetic shaking, it was found that the reaction time decreased with magnetic shaking for all CRP concentrations. Even after the magnetic-shaking treatment, the dependence of the CRP concentration on the magnetic signal was observed. The number of surface modifications per magnetic marker and the magnetic marker concentration also affected the reaction time. In order to obtain the desired measurement range based on the CRP response characteristic, it is necessary to optimize the number of magnetic markers and polymer beads used. |
| first_indexed | 2025-11-15T02:37:22Z |
| format | Article |
| id | ump-25077 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:37:22Z |
| publishDate | 2019 |
| publisher | IEEE |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-250772019-07-01T04:54:50Z http://umpir.ump.edu.my/id/eprint/25077/ Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay Tsukada, Keiji Tsunashima, Kenta Jinno, Katsuya Hiramatsu, Bunta Takeuchi, Shun Fujimoto, Kayo Kenji, Sakai Kiwa, Toshihiko Mohd Mawardi, Saari Q Science (General) R Medicine (General) The measurement of biological targets using nanoparticle magnetic markers has been extensively studied for its applications in magnetic immunoassays (MIAs). Compared with the optical immunoassay method, the MIA methods have some attractive characteristics such as a wash-free process that does not need bound/free marker separation and the ability to detect biological targets in a nebulous liquid. In addition, the wash-free process is very fast. In this paper, we tried to reduce the reaction time for the antigen-antibody reaction in order to make the MIA method more attractive. The reduction in reaction time and the detection characteristic of biological targets were demonstrated using C-reactive proteins (CRPs) as biological targets for diagnosing inflammation. To shorten the reaction time, a magnetic-shaking method using the magnetic field gradient from a neodymium magnet was developed. When comparing the reaction times with and without magnetic shaking, it was found that the reaction time decreased with magnetic shaking for all CRP concentrations. Even after the magnetic-shaking treatment, the dependence of the CRP concentration on the magnetic signal was observed. The number of surface modifications per magnetic marker and the magnetic marker concentration also affected the reaction time. In order to obtain the desired measurement range based on the CRP response characteristic, it is necessary to optimize the number of magnetic markers and polymer beads used. IEEE 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25077/1/Using%20Magnetic%20Field%20Gradients%20to%20Shorten%20the%20Antigen1.pdf Tsukada, Keiji and Tsunashima, Kenta and Jinno, Katsuya and Hiramatsu, Bunta and Takeuchi, Shun and Fujimoto, Kayo and Kenji, Sakai and Kiwa, Toshihiko and Mohd Mawardi, Saari (2019) Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay. IEEE Transactions on Magnetics, 55 (7). pp. 1-5. ISSN 0018-9464. (Published) https://doi.org/10.1109/TMAG.2019.2894904 https://doi.org/10.1109/TMAG.2019.2894904 |
| spellingShingle | Q Science (General) R Medicine (General) Tsukada, Keiji Tsunashima, Kenta Jinno, Katsuya Hiramatsu, Bunta Takeuchi, Shun Fujimoto, Kayo Kenji, Sakai Kiwa, Toshihiko Mohd Mawardi, Saari Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay |
| title | Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay |
| title_full | Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay |
| title_fullStr | Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay |
| title_full_unstemmed | Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay |
| title_short | Using Magnetic Field Gradients to Shorten the Antigen-Antibody Reaction Time for a Magnetic Immunoassay |
| title_sort | using magnetic field gradients to shorten the antigen-antibody reaction time for a magnetic immunoassay |
| topic | Q Science (General) R Medicine (General) |
| url | http://umpir.ump.edu.my/id/eprint/25077/ http://umpir.ump.edu.my/id/eprint/25077/ http://umpir.ump.edu.my/id/eprint/25077/ http://umpir.ump.edu.my/id/eprint/25077/1/Using%20Magnetic%20Field%20Gradients%20to%20Shorten%20the%20Antigen1.pdf |