Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry
The objective of this research was to perform extraction of propolis using water as extraction solvent and analyze the propolis extract by using Thin Layer Chromatography (TLC), High Performance liquid Chromatography (HPLC) and Ultraviolet–Visible (UV-Vis) Spectrophotometry. The effect of propolis t...
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| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/24521/ http://umpir.ump.edu.my/id/eprint/24521/1/Extraction%20of%20propolis%20by%20using%20water%20and%20evaluation%20by%20TLC.pdf |
| _version_ | 1848822053956747264 |
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| author | Tan, Darlene Tzet Lene |
| author_facet | Tan, Darlene Tzet Lene |
| author_sort | Tan, Darlene Tzet Lene |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The objective of this research was to perform extraction of propolis using water as extraction solvent and analyze the propolis extract by using Thin Layer Chromatography (TLC), High Performance liquid Chromatography (HPLC) and Ultraviolet–Visible (UV-Vis) Spectrophotometry. The effect of propolis to solvent ratio was determined by increasing the ratio from 1:5, 1:10 to 1:15 where the extraction yield of extract were 5.95%, 9.05% and 7.16% respectively. Larger ratio increases extract yield, however, extract yield at ratio larger 1:10 will not further increase percentage. Analysis by UV-Vis Spectrophotometry was carried out to determine the total flavone, flavonols and total phenolic contents in Water Extracted Propolis (WEP). Both contents showed a highest concentration in the 1:5 ratio, which were 3.33 mg Quercetin Equivalent (QE)/g and 6.12 mg Gallic Acid Equivalent (GAE)/g respectively. TLC analysis detected caffeic acid components in WEP at |
| first_indexed | 2025-11-15T02:35:08Z |
| format | Undergraduates Project Papers |
| id | ump-24521 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:35:08Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-245212023-11-20T07:56:59Z http://umpir.ump.edu.my/id/eprint/24521/ Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry Tan, Darlene Tzet Lene T Technology (General) The objective of this research was to perform extraction of propolis using water as extraction solvent and analyze the propolis extract by using Thin Layer Chromatography (TLC), High Performance liquid Chromatography (HPLC) and Ultraviolet–Visible (UV-Vis) Spectrophotometry. The effect of propolis to solvent ratio was determined by increasing the ratio from 1:5, 1:10 to 1:15 where the extraction yield of extract were 5.95%, 9.05% and 7.16% respectively. Larger ratio increases extract yield, however, extract yield at ratio larger 1:10 will not further increase percentage. Analysis by UV-Vis Spectrophotometry was carried out to determine the total flavone, flavonols and total phenolic contents in Water Extracted Propolis (WEP). Both contents showed a highest concentration in the 1:5 ratio, which were 3.33 mg Quercetin Equivalent (QE)/g and 6.12 mg Gallic Acid Equivalent (GAE)/g respectively. TLC analysis detected caffeic acid components in WEP at 2017-12 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24521/1/Extraction%20of%20propolis%20by%20using%20water%20and%20evaluation%20by%20TLC.pdf Tan, Darlene Tzet Lene (2017) Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry. Faculty of Engineering Technology, Universiti Malaysia Pahang. http://ecollib.ump.edu.my/36216/ |
| spellingShingle | T Technology (General) Tan, Darlene Tzet Lene Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry |
| title | Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry |
| title_full | Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry |
| title_fullStr | Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry |
| title_full_unstemmed | Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry |
| title_short | Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry |
| title_sort | extraction of propolis by using water and evaluation by tlc, hplc and uv vis spectrophotometry |
| topic | T Technology (General) |
| url | http://umpir.ump.edu.my/id/eprint/24521/ http://umpir.ump.edu.my/id/eprint/24521/ http://umpir.ump.edu.my/id/eprint/24521/1/Extraction%20of%20propolis%20by%20using%20water%20and%20evaluation%20by%20TLC.pdf |