Optimization of protein yields in water extracts of Jackiopsis ornata roots by response surface methodology using microwave assisted extraction (MAE)

Optimization of protein yields in water extracts that were obtained from Jackiopsis ornataroots by Microwave assisted extraction (MAE) was established by a Circumscribed Central Composite Design (CCCD). MAE proved to be an optimum choice for the protein extraction. The highest yield (20.425±108) was...

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Main Authors: Elhag, Husam Eldin Elhag Abugabr, Nailaa, Aishath, Azilah, Ajit, W. F. W., Ishak, A. Z., Sulaiman, Badhrulhisham, Abdul Aziz, Prasad, D. M. Reddy
Format: Conference or Workshop Item
Language:English
Published: AIP Publishing 2019
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/24315/
http://umpir.ump.edu.my/id/eprint/24315/7/Optimization%20of%20protein%20yields%20in%20water%20extracts1.pdf
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author Elhag, Husam Eldin Elhag Abugabr
Nailaa, Aishath
Azilah, Ajit
W. F. W., Ishak
A. Z., Sulaiman
Badhrulhisham, Abdul Aziz
Prasad, D. M. Reddy
author_facet Elhag, Husam Eldin Elhag Abugabr
Nailaa, Aishath
Azilah, Ajit
W. F. W., Ishak
A. Z., Sulaiman
Badhrulhisham, Abdul Aziz
Prasad, D. M. Reddy
author_sort Elhag, Husam Eldin Elhag Abugabr
building UMP Institutional Repository
collection Online Access
description Optimization of protein yields in water extracts that were obtained from Jackiopsis ornataroots by Microwave assisted extraction (MAE) was established by a Circumscribed Central Composite Design (CCCD). MAE proved to be an optimum choice for the protein extraction. The highest yield (20.425±108) was obtained with the parameters liquid to solid ratio (S= 30:1), particle size (P=0.022±0.002mm (radius), temperature (T= 65°C); microwave power (W= 300W) and extraction time (t= 20 minutes). Statistical analysis illustrated the adequacy of the generated full quadratic equation with ad high values of the coefficient of determination (R2), adjusted coefficient of determination R2adj and predicted coefficient p of determination R2pred The p value of the Lack of Fit test illustrated the adequacy of the model. The optimization conditions of extracting the protein was predicted to be with the parameters S: 26, P: 0.0284; T: 65 °C; P: 303 W and t: 20 min. The high efficiency of MAE to obtain J. ornataprotein extract encouraged further application on pilot and industrial scale.
first_indexed 2025-11-15T02:34:16Z
format Conference or Workshop Item
id ump-24315
institution Universiti Malaysia Pahang
institution_category Local University
language English
last_indexed 2025-11-15T02:34:16Z
publishDate 2019
publisher AIP Publishing
recordtype eprints
repository_type Digital Repository
spelling ump-243152020-06-26T03:19:54Z http://umpir.ump.edu.my/id/eprint/24315/ Optimization of protein yields in water extracts of Jackiopsis ornata roots by response surface methodology using microwave assisted extraction (MAE) Elhag, Husam Eldin Elhag Abugabr Nailaa, Aishath Azilah, Ajit W. F. W., Ishak A. Z., Sulaiman Badhrulhisham, Abdul Aziz Prasad, D. M. Reddy TP Chemical technology Optimization of protein yields in water extracts that were obtained from Jackiopsis ornataroots by Microwave assisted extraction (MAE) was established by a Circumscribed Central Composite Design (CCCD). MAE proved to be an optimum choice for the protein extraction. The highest yield (20.425±108) was obtained with the parameters liquid to solid ratio (S= 30:1), particle size (P=0.022±0.002mm (radius), temperature (T= 65°C); microwave power (W= 300W) and extraction time (t= 20 minutes). Statistical analysis illustrated the adequacy of the generated full quadratic equation with ad high values of the coefficient of determination (R2), adjusted coefficient of determination R2adj and predicted coefficient p of determination R2pred The p value of the Lack of Fit test illustrated the adequacy of the model. The optimization conditions of extracting the protein was predicted to be with the parameters S: 26, P: 0.0284; T: 65 °C; P: 303 W and t: 20 min. The high efficiency of MAE to obtain J. ornataprotein extract encouraged further application on pilot and industrial scale. AIP Publishing 2019 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24315/7/Optimization%20of%20protein%20yields%20in%20water%20extracts1.pdf Elhag, Husam Eldin Elhag Abugabr and Nailaa, Aishath and Azilah, Ajit and W. F. W., Ishak and A. Z., Sulaiman and Badhrulhisham, Abdul Aziz and Prasad, D. M. Reddy (2019) Optimization of protein yields in water extracts of Jackiopsis ornata roots by response surface methodology using microwave assisted extraction (MAE). In: AIP Conference Proceedings: Materials Characterization Using X-Rays and Related Techniques , 18-19 August 2018 , Kota Bharu, Kelantan, Malaysia. pp. 1-10., 2068 (020055). ISBN 978-0-7354-1796-0 (Published) https://aip.scitation.org/doi/abs/10.1063/1.5089354
spellingShingle TP Chemical technology
Elhag, Husam Eldin Elhag Abugabr
Nailaa, Aishath
Azilah, Ajit
W. F. W., Ishak
A. Z., Sulaiman
Badhrulhisham, Abdul Aziz
Prasad, D. M. Reddy
Optimization of protein yields in water extracts of Jackiopsis ornata roots by response surface methodology using microwave assisted extraction (MAE)
title Optimization of protein yields in water extracts of Jackiopsis ornata roots by response surface methodology using microwave assisted extraction (MAE)
title_full Optimization of protein yields in water extracts of Jackiopsis ornata roots by response surface methodology using microwave assisted extraction (MAE)
title_fullStr Optimization of protein yields in water extracts of Jackiopsis ornata roots by response surface methodology using microwave assisted extraction (MAE)
title_full_unstemmed Optimization of protein yields in water extracts of Jackiopsis ornata roots by response surface methodology using microwave assisted extraction (MAE)
title_short Optimization of protein yields in water extracts of Jackiopsis ornata roots by response surface methodology using microwave assisted extraction (MAE)
title_sort optimization of protein yields in water extracts of jackiopsis ornata roots by response surface methodology using microwave assisted extraction (mae)
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/24315/
http://umpir.ump.edu.my/id/eprint/24315/
http://umpir.ump.edu.my/id/eprint/24315/7/Optimization%20of%20protein%20yields%20in%20water%20extracts1.pdf