Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane
Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into cyclodextrin (CD). However, the instability of the enzyme during the reaction process results in the low production of CD. Immobilization process has been pro...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
Elsevier Ltd
2018
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/23883/ http://umpir.ump.edu.my/id/eprint/23883/1/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf http://umpir.ump.edu.my/id/eprint/23883/7/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase.pdf |
| _version_ | 1848821911103995904 |
|---|---|
| author | Natassha, Jamil Rohaida, Che Man Suhaily, Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman |
| author_facet | Natassha, Jamil Rohaida, Che Man Suhaily, Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman |
| author_sort | Natassha, Jamil |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into cyclodextrin (CD). However, the instability of the enzyme during the reaction process results in the low production of CD. Immobilization process has been proven as a promising method to improve the enzyme stability in order to achieve the high production of CD. In this study, the CGTase from Bacillus licheniformis was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane by adsorption. The effects of the enzyme concentration [0.6-1.4% (v/v)] and temperature (20-40 °C) on the immobilization yield were investigated. The highest immobilization yield of 80.0% was observed when 10% (v/v) of enzyme concentration was used. The optimal immobilization temperature obtained was at 25 °C with 95.6%
of immobilization yield. Hence, the high immobilization yield contributed to the high production of CD which may be beneficial for the industrial purposes. |
| first_indexed | 2025-11-15T02:32:52Z |
| format | Conference or Workshop Item |
| id | ump-23883 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T02:32:52Z |
| publishDate | 2018 |
| publisher | Elsevier Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-238832019-05-17T04:00:43Z http://umpir.ump.edu.my/id/eprint/23883/ Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane Natassha, Jamil Rohaida, Che Man Suhaily, Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Q Science (General) Cyclodextrin glucanotransferase (CGTase) is a multifunctional industrial enzyme, which undergoes cyclization reaction to converts starch into cyclodextrin (CD). However, the instability of the enzyme during the reaction process results in the low production of CD. Immobilization process has been proven as a promising method to improve the enzyme stability in order to achieve the high production of CD. In this study, the CGTase from Bacillus licheniformis was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane by adsorption. The effects of the enzyme concentration [0.6-1.4% (v/v)] and temperature (20-40 °C) on the immobilization yield were investigated. The highest immobilization yield of 80.0% was observed when 10% (v/v) of enzyme concentration was used. The optimal immobilization temperature obtained was at 25 °C with 95.6% of immobilization yield. Hence, the high immobilization yield contributed to the high production of CD which may be beneficial for the industrial purposes. Elsevier Ltd 2018 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23883/1/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf pdf en http://umpir.ump.edu.my/id/eprint/23883/7/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase.pdf Natassha, Jamil and Rohaida, Che Man and Suhaily, Suhaimi and Shalyda, Md Shaarani and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman (2018) Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane. In: The 3rd International Conference on Green Chemical Engineering and Technology (3rd GCET) , 7-8 November 2017 , Melaka, Malaysia. pp. 22036-22042., 5 (10). ISSN 2214-7853 (Published) https://doi.org/10.1016/j.matpr.2018.07.065 10.1016/j.matpr.2018.07.065 |
| spellingShingle | Q Science (General) Natassha, Jamil Rohaida, Che Man Suhaily, Suhaimi Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane |
| title | Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane |
| title_full | Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane |
| title_fullStr | Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane |
| title_full_unstemmed | Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane |
| title_short | Effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (CGTase) on hollow fiber membrane |
| title_sort | effect of enzyme concentration and temperature on the immobilization of cyclodextrin glucanotransferase (cgtase) on hollow fiber membrane |
| topic | Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/23883/ http://umpir.ump.edu.my/id/eprint/23883/ http://umpir.ump.edu.my/id/eprint/23883/ http://umpir.ump.edu.my/id/eprint/23883/1/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29%20on%20hollow%20fiber%20membrane.pdf http://umpir.ump.edu.my/id/eprint/23883/7/Effect%20of%20enzyme%20concentration%20and%20temperature%20on%20the%20immobilization%20of%20cyclodextrin%20glucanotransferase.pdf |