Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx
This work reports on a novel glucose biosensor based on co-immobilization of glucose oxidase (GOx) and horseradish peroxidase with polymerized multiporous nanofiber (MPNFs) of SnO2 onto glassy carbon electrode with chitosan. Multiporous nanofibers of SnO2 were synthesized by electrospinning method f...
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| Format: | Article |
| Language: | English |
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American Scientific Publishers
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/23537/ http://umpir.ump.edu.my/id/eprint/23537/1/Application%20of%20polymerized%20multiporous%20nanofiber%20of%20SnO2%20for%20designing.pdf |
| _version_ | 1848821828074602496 |
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| author | A. K. M., Kafi Rajan, Jose M. M., Yusoff Samiul, Alim |
| author_facet | A. K. M., Kafi Rajan, Jose M. M., Yusoff Samiul, Alim |
| author_sort | A. K. M., Kafi |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | This work reports on a novel glucose biosensor based on co-immobilization of glucose oxidase (GOx) and horseradish peroxidase with polymerized multiporous nanofiber (MPNFs) of SnO2 onto glassy carbon electrode with chitosan. Multiporous nanofibers of SnO2 were synthesized by electrospinning method from the tin precursor which possesses high surface area good electrical conductivity, and the nanofibers were polymerized with polyaniline (PANI). GOx and HRP were then co-immobilized with the nanofibers on the surface of the glassy carbon electrode by using chitosan. The polymerized nanofibers play a significant role in facilitating the direct electron transfer between the electroactive center of the immobilized enzyme and the electrode surface. The morphology of the nanofiber and polymerized nanofiber has been evaluated by field emission scanning electron microscopy (FESEM). Cyclic Voltammetry and amperometry were employed to study and optimize the performance of the fabricated biosensor. The PANI/SnO2-NF/GOx-HRP/Ch/GC biosensor displayed a linear amperometric response towards the glucose concentration range from 5 to 100 μM with a detection limit of 1.8 μM (S/N = 3). Also, the anti-interference study and real sample analysis was investigated. Furthermore, the biosensor reported in this work exhibited excellent stability, reproducibility, and repeatability |
| first_indexed | 2025-11-15T02:31:32Z |
| format | Article |
| id | ump-23537 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:31:32Z |
| publishDate | 2019 |
| publisher | American Scientific Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-235372019-01-29T01:26:37Z http://umpir.ump.edu.my/id/eprint/23537/ Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx A. K. M., Kafi Rajan, Jose M. M., Yusoff Samiul, Alim T Technology (General) This work reports on a novel glucose biosensor based on co-immobilization of glucose oxidase (GOx) and horseradish peroxidase with polymerized multiporous nanofiber (MPNFs) of SnO2 onto glassy carbon electrode with chitosan. Multiporous nanofibers of SnO2 were synthesized by electrospinning method from the tin precursor which possesses high surface area good electrical conductivity, and the nanofibers were polymerized with polyaniline (PANI). GOx and HRP were then co-immobilized with the nanofibers on the surface of the glassy carbon electrode by using chitosan. The polymerized nanofibers play a significant role in facilitating the direct electron transfer between the electroactive center of the immobilized enzyme and the electrode surface. The morphology of the nanofiber and polymerized nanofiber has been evaluated by field emission scanning electron microscopy (FESEM). Cyclic Voltammetry and amperometry were employed to study and optimize the performance of the fabricated biosensor. The PANI/SnO2-NF/GOx-HRP/Ch/GC biosensor displayed a linear amperometric response towards the glucose concentration range from 5 to 100 μM with a detection limit of 1.8 μM (S/N = 3). Also, the anti-interference study and real sample analysis was investigated. Furthermore, the biosensor reported in this work exhibited excellent stability, reproducibility, and repeatability American Scientific Publishers 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23537/1/Application%20of%20polymerized%20multiporous%20nanofiber%20of%20SnO2%20for%20designing.pdf A. K. M., Kafi and Rajan, Jose and M. M., Yusoff and Samiul, Alim (2019) Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx. International Journal of Biological Macromolecules, 123. pp. 1028-1034. (Published) http://www.elsevier.com/locate/ijbiomac https://doi.org/10.1166/jnn.2019.15465 |
| spellingShingle | T Technology (General) A. K. M., Kafi Rajan, Jose M. M., Yusoff Samiul, Alim Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx |
| title | Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx |
| title_full | Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx |
| title_fullStr | Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx |
| title_full_unstemmed | Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx |
| title_short | Application of polymerized multiporous nanofiber of SnO2 for designing a bienzyme glucose biosensor based on HRP/GOx |
| title_sort | application of polymerized multiporous nanofiber of sno2 for designing a bienzyme glucose biosensor based on hrp/gox |
| topic | T Technology (General) |
| url | http://umpir.ump.edu.my/id/eprint/23537/ http://umpir.ump.edu.my/id/eprint/23537/ http://umpir.ump.edu.my/id/eprint/23537/ http://umpir.ump.edu.my/id/eprint/23537/1/Application%20of%20polymerized%20multiporous%20nanofiber%20of%20SnO2%20for%20designing.pdf |