Effect of freeze and thaw cycle and incubation period on the solubilisation of inclusion body protein

Effect of freeze and thaw cycle and incubation period on the solubilisation of inclusion body protein

Overexpression of recombinant protein in bacteria result in the formation of inactive protein. These inactive proteins associate forming insoluble protein aggregates which is referring to inclusion bodies (IBs). Generally, IBs are pure and the aggregated protein inside it has native-like secondary s...

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Bibliographic Details
Main Author: Nursyahira, Mohd Rafi
Format: Undergraduates Project Papers
Language:English
Published: 2017
Subjects:
QD Chemistry
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/22315/
http://umpir.ump.edu.my/id/eprint/22315/1/Effect%20of%20freeze%20and%20thaw%20cycle%20and%20incubation%20period%20on%20the%20solubilisation%20of%20inclusion%20body%20protein.pdf

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