Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility
Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent...
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/21739/ http://umpir.ump.edu.my/id/eprint/21739/1/Statistical%20optimization%20of%20gelatin%20immobilisation.pdf |
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| author | Nurhusna, Samsudin Yumi Zuhanis Has Yun, Hashim Mohd Azmir, Arifin Maizirwan, Mel Hamzah, Mohd. Salleh Norshariza, Nordin Sopyan, Iis Dzun Noraini, Jimat |
| author_facet | Nurhusna, Samsudin Yumi Zuhanis Has Yun, Hashim Mohd Azmir, Arifin Maizirwan, Mel Hamzah, Mohd. Salleh Norshariza, Nordin Sopyan, Iis Dzun Noraini, Jimat |
| author_sort | Nurhusna, Samsudin |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of 16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105 cells/ml) and UV/O3 treated PCL microcarrier (4.25×105 cells/ml). Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier. |
| first_indexed | 2025-11-15T02:25:03Z |
| format | Article |
| id | ump-21739 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:25:03Z |
| publishDate | 2017 |
| publisher | Penerbit UTM Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-217392024-01-10T07:27:30Z http://umpir.ump.edu.my/id/eprint/21739/ Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility Nurhusna, Samsudin Yumi Zuhanis Has Yun, Hashim Mohd Azmir, Arifin Maizirwan, Mel Hamzah, Mohd. Salleh Norshariza, Nordin Sopyan, Iis Dzun Noraini, Jimat T Technology (General) Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of 16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105 cells/ml) and UV/O3 treated PCL microcarrier (4.25×105 cells/ml). Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier. Penerbit UTM Press 2017-09 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/21739/1/Statistical%20optimization%20of%20gelatin%20immobilisation.pdf Nurhusna, Samsudin and Yumi Zuhanis Has Yun, Hashim and Mohd Azmir, Arifin and Maizirwan, Mel and Hamzah, Mohd. Salleh and Norshariza, Nordin and Sopyan, Iis and Dzun Noraini, Jimat (2017) Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility. Jurnal Teknologi (Sciences & Engineering), 79 (6). pp. 167-175. ISSN 2180–3722. (Published) http://dx.doi.org/10.11113/jt.v79.10417 http://dx.doi.org/10.11113/jt.v79.10417 |
| spellingShingle | T Technology (General) Nurhusna, Samsudin Yumi Zuhanis Has Yun, Hashim Mohd Azmir, Arifin Maizirwan, Mel Hamzah, Mohd. Salleh Norshariza, Nordin Sopyan, Iis Dzun Noraini, Jimat Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title | Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title_full | Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title_fullStr | Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title_full_unstemmed | Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title_short | Statistical optimization of gelatin immobilisation on modified surface PCL microcarrier to improve PCL microcarrier compatibility |
| title_sort | statistical optimization of gelatin immobilisation on modified surface pcl microcarrier to improve pcl microcarrier compatibility |
| topic | T Technology (General) |
| url | http://umpir.ump.edu.my/id/eprint/21739/ http://umpir.ump.edu.my/id/eprint/21739/ http://umpir.ump.edu.my/id/eprint/21739/ http://umpir.ump.edu.my/id/eprint/21739/1/Statistical%20optimization%20of%20gelatin%20immobilisation.pdf |