Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria
Objectives: To make preliminary screening study for the presence of ESBL producing bacteria, in food and drinks sold in Gombe metropolis. 30 samples of food and drinks were collected and analyzed. Method: Bacterial isolation was performed on nutrient agar and identification started from gram stainin...
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| Format: | Article |
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Informatics Publishing Limited
2016
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| Online Access: | http://umpir.ump.edu.my/id/eprint/21408/ http://umpir.ump.edu.my/id/eprint/21408/1/Assessment%20of%20Foods%20and%20Drinks%20for%20the%20Presence.pdf |
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| author | Shu’aibu, I. Hadiza, J. A. Yusha’u, M. Kabiru, M. Y. Ahmad, Mani Malam Lawal, G. Adamu, M. T. Khairiyya, M. |
| author_facet | Shu’aibu, I. Hadiza, J. A. Yusha’u, M. Kabiru, M. Y. Ahmad, Mani Malam Lawal, G. Adamu, M. T. Khairiyya, M. |
| author_sort | Shu’aibu, I. |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Objectives: To make preliminary screening study for the presence of ESBL producing bacteria, in food and drinks sold in Gombe metropolis. 30 samples of food and drinks were collected and analyzed. Method: Bacterial isolation was performed on nutrient agar and identification started from gram staining; the gram negative isolates obtained, among which ESBL producers were found and further subjected to standard biochemical tests and confirmed to be enterobacteriaceae. They were then screened for ESBLs in accordance with Clinical and Laboratory Standard Institute (CLSI) breakpoint, and the suspected ESBLs bacteria were confirmed using double disc synergy testing (DDST) with standard Augmentin (Amoxycillin/ Clavulanic Acid, 30μg; Oxoid England, CT0223B), Ceftriaxone (CRO 30μg; Oxoid England, CT0417B) and Ceftazidime (CAZ 30μg; Oxoid England, CT0412B). Findings: The fourteen 14(100%) Gram negative bacteria isolated were found to include Pseudomonasaeruginosa 2(14.29%) and members of the family Enterobacteriaceae (enteric gram negative rods): Citrobacterfreundii 2(14.29%), Enterobacterspp. 1(7.14%), Escherichiacoli 4(28.57%), Klebsiellapneumoniae 3(21.43%), Proteusvulgaris 1(7.14%) and Salmonellatyphi 1(7.14%). When screened for ESBL production based on CLSI breakpoint, only 6 (49.99%) were positive and included C.freundii 1(7.14%), E.coli 2(14.29%), K.pneumoniae 1(7.14%), S. typhi 1(7.14%) and 1(7.14%) for Ps. aeruginosa. On subjecting them to DDST, only 2(33.34%) out of them were found to be ESBLs positive by an increase in inhibition zone of 5mm toward the centre disc, Augmentin. They include E. coli 1(16.67%) and S. typhi 1(16.67%). Application: In a nutshell, foods and drinks sold therein could serve as reservoirs for ESBLs producing bacteria. |
| first_indexed | 2025-11-15T02:23:49Z |
| format | Article |
| id | ump-21408 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:23:49Z |
| publishDate | 2016 |
| publisher | Informatics Publishing Limited |
| recordtype | eprints |
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| spelling | ump-214082018-06-27T08:12:51Z http://umpir.ump.edu.my/id/eprint/21408/ Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria Shu’aibu, I. Hadiza, J. A. Yusha’u, M. Kabiru, M. Y. Ahmad, Mani Malam Lawal, G. Adamu, M. T. Khairiyya, M. TP Chemical technology Objectives: To make preliminary screening study for the presence of ESBL producing bacteria, in food and drinks sold in Gombe metropolis. 30 samples of food and drinks were collected and analyzed. Method: Bacterial isolation was performed on nutrient agar and identification started from gram staining; the gram negative isolates obtained, among which ESBL producers were found and further subjected to standard biochemical tests and confirmed to be enterobacteriaceae. They were then screened for ESBLs in accordance with Clinical and Laboratory Standard Institute (CLSI) breakpoint, and the suspected ESBLs bacteria were confirmed using double disc synergy testing (DDST) with standard Augmentin (Amoxycillin/ Clavulanic Acid, 30μg; Oxoid England, CT0223B), Ceftriaxone (CRO 30μg; Oxoid England, CT0417B) and Ceftazidime (CAZ 30μg; Oxoid England, CT0412B). Findings: The fourteen 14(100%) Gram negative bacteria isolated were found to include Pseudomonasaeruginosa 2(14.29%) and members of the family Enterobacteriaceae (enteric gram negative rods): Citrobacterfreundii 2(14.29%), Enterobacterspp. 1(7.14%), Escherichiacoli 4(28.57%), Klebsiellapneumoniae 3(21.43%), Proteusvulgaris 1(7.14%) and Salmonellatyphi 1(7.14%). When screened for ESBL production based on CLSI breakpoint, only 6 (49.99%) were positive and included C.freundii 1(7.14%), E.coli 2(14.29%), K.pneumoniae 1(7.14%), S. typhi 1(7.14%) and 1(7.14%) for Ps. aeruginosa. On subjecting them to DDST, only 2(33.34%) out of them were found to be ESBLs positive by an increase in inhibition zone of 5mm toward the centre disc, Augmentin. They include E. coli 1(16.67%) and S. typhi 1(16.67%). Application: In a nutshell, foods and drinks sold therein could serve as reservoirs for ESBLs producing bacteria. Informatics Publishing Limited 2016 Article PeerReviewed text en cc_by http://umpir.ump.edu.my/id/eprint/21408/1/Assessment%20of%20Foods%20and%20Drinks%20for%20the%20Presence.pdf Shu’aibu, I. and Hadiza, J. A. and Yusha’u, M. and Kabiru, M. Y. and Ahmad, Mani Malam and Lawal, G. and Adamu, M. T. and Khairiyya, M. (2016) Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria. Indian Journal of Science and Technology, 9 (48). pp. 1-9. ISSN 0974-6846 (Print); 0974-5645 (Online). (Published) http://dx.doi.org/10.17485/ijst%2F2016%2Fv9i48%2F109624 DOI: 10.17485/ijst/2016/v9i48/109624 |
| spellingShingle | TP Chemical technology Shu’aibu, I. Hadiza, J. A. Yusha’u, M. Kabiru, M. Y. Ahmad, Mani Malam Lawal, G. Adamu, M. T. Khairiyya, M. Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria |
| title | Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria |
| title_full | Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria |
| title_fullStr | Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria |
| title_full_unstemmed | Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria |
| title_short | Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria |
| title_sort | assessment of foods and drinks for the presence of extended spectrum beta lactamase (esbl) producing bacteria in gombe metropolis, nigeria |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/21408/ http://umpir.ump.edu.my/id/eprint/21408/ http://umpir.ump.edu.my/id/eprint/21408/ http://umpir.ump.edu.my/id/eprint/21408/1/Assessment%20of%20Foods%20and%20Drinks%20for%20the%20Presence.pdf |