Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation
This study aims to produce succinic acid from glycerol residue through anaerobic fermentation process. The optimum condition of pH, mass substrate, and temperature was determined using response surface methodology (RSM). The concentration of glycerol and succinic acid were determined us...
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| Format: | Article |
| Language: | English |
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Universiti Malaysia Pahang
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/19138/ http://umpir.ump.edu.my/id/eprint/19138/1/fkksa-2017-mimi-Optimization%20Of%20Glycerol%20Recovery%20From%20Pretreatment.pdf |
| _version_ | 1848820840170258432 |
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| author | Nik Nor Aziati, Abdul Aziz Mimi Sakinah, A. M. |
| author_facet | Nik Nor Aziati, Abdul Aziz Mimi Sakinah, A. M. |
| author_sort | Nik Nor Aziati, Abdul Aziz |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | This study aims to produce succinic acid from glycerol residue through anaerobic fermentation process. The optimum condition of pH, mass substrate, and temperature was determined using response surface methodology (RSM). The concentration of glycerol and succinic acid were determined using HPLC analysis. The FTIR spectrometry was applied to examine the adsorption of organic element. Optimization of fermentation condition using RSM yielded glycerol of 159.312 g/L at pH 2, 35 °C, and 110.36 g of substrate. Succinic acid was the product of fermentation by Escherichia coli type k-12 under 19.67 g/L initial glycerol concentration, 200 rpm rotational speed, and 37 °C. The fermentation with treated glycerol generated 0.66983 g/L of succinic acid, compared to commercial glycerol which generated 0.73337 g/L of succinic acid. Overall, this research show that glycerol waste can be used as the carbon source to produce succinic acid by implementing fermentation of Escherichia coli type k-12. From the fermentation process, succinic acid was not the major product. Further study in genetic modification of Escherichia coli is highly suggested to produce succinic acid as the major product, as well as to improve the fermentation process. |
| first_indexed | 2025-11-15T02:15:50Z |
| format | Article |
| id | ump-19138 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:15:50Z |
| publishDate | 2017 |
| publisher | Universiti Malaysia Pahang |
| recordtype | eprints |
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| spelling | ump-191382017-11-21T03:41:46Z http://umpir.ump.edu.my/id/eprint/19138/ Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation Nik Nor Aziati, Abdul Aziz Mimi Sakinah, A. M. TP Chemical technology This study aims to produce succinic acid from glycerol residue through anaerobic fermentation process. The optimum condition of pH, mass substrate, and temperature was determined using response surface methodology (RSM). The concentration of glycerol and succinic acid were determined using HPLC analysis. The FTIR spectrometry was applied to examine the adsorption of organic element. Optimization of fermentation condition using RSM yielded glycerol of 159.312 g/L at pH 2, 35 °C, and 110.36 g of substrate. Succinic acid was the product of fermentation by Escherichia coli type k-12 under 19.67 g/L initial glycerol concentration, 200 rpm rotational speed, and 37 °C. The fermentation with treated glycerol generated 0.66983 g/L of succinic acid, compared to commercial glycerol which generated 0.73337 g/L of succinic acid. Overall, this research show that glycerol waste can be used as the carbon source to produce succinic acid by implementing fermentation of Escherichia coli type k-12. From the fermentation process, succinic acid was not the major product. Further study in genetic modification of Escherichia coli is highly suggested to produce succinic acid as the major product, as well as to improve the fermentation process. Universiti Malaysia Pahang 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/19138/1/fkksa-2017-mimi-Optimization%20Of%20Glycerol%20Recovery%20From%20Pretreatment.pdf Nik Nor Aziati, Abdul Aziz and Mimi Sakinah, A. M. (2017) Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation. Journal of Chemical Engineering and Industrial Biotechnology (JCEIB), 2. pp. 27-36. ISSN 0126-8139. (Published) http://jceib.ump.edu.my/index.php/en/download/volume2-2017/41-optimization-of-glycerol-recovery-from-pretreatment-process-to-produce-succinic-acid-via-anaerobic-fermentation-page-27-36/file DOI: https://doi.org/10.15282/JCEIB-V2-01.29/9/2017/2.2 |
| spellingShingle | TP Chemical technology Nik Nor Aziati, Abdul Aziz Mimi Sakinah, A. M. Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation |
| title | Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation |
| title_full | Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation |
| title_fullStr | Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation |
| title_full_unstemmed | Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation |
| title_short | Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation |
| title_sort | optimization of glycerol recovery from pretreatment process to produce succinic acid via anaerobic fermentation |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/19138/ http://umpir.ump.edu.my/id/eprint/19138/ http://umpir.ump.edu.my/id/eprint/19138/ http://umpir.ump.edu.my/id/eprint/19138/1/fkksa-2017-mimi-Optimization%20Of%20Glycerol%20Recovery%20From%20Pretreatment.pdf |