Ultrasonic Assisted Extraction of Phenolic and Flavonoid Content from Orthosiphon Stamineus Leaves

This paper presents a combined effect of ultrasonic assisted extraction and solvents of varying polarities on phenolic compound extraction from O. stamineus. The polyphenol content in the plant extracts was analysed using Singleton’s method and aluminium chloride colorimetric assay. The results sugg...

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Main Authors: Sook, Fun Pang, M. M., Yusoff, Luqman Chuah, Abdullah, Jolius, Gimbun
Format: Article
Language:English
Published: Asia Pacific Publishing 2015
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/18447/
http://umpir.ump.edu.my/id/eprint/18447/1/70%20STP%201%2010-13.pdf
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author Sook, Fun Pang
M. M., Yusoff
Luqman Chuah, Abdullah
Jolius, Gimbun
author_facet Sook, Fun Pang
M. M., Yusoff
Luqman Chuah, Abdullah
Jolius, Gimbun
author_sort Sook, Fun Pang
building UMP Institutional Repository
collection Online Access
description This paper presents a combined effect of ultrasonic assisted extraction and solvents of varying polarities on phenolic compound extraction from O. stamineus. The polyphenol content in the plant extracts was analysed using Singleton’s method and aluminium chloride colorimetric assay. The results suggest that the polyphenol extraction from O. stamineus is affected by the solvent type. The highest phenolic content of 168.8 mg GAE/g DW was obtained from ultrasonic assisted extraction (UAE) using 70% aqueous methanol and 70% aqueous propanol solvent. The highest total flavonoid content of 185.3 mg QE/g DW was obtained using 70% aqueous propanol. The phenolic acid and flavonoid yield increased with extraction time, however, extraction beyond 120 min or at a temperature higher than 60°C induced degradation and hence reducing extraction yield.
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institution Universiti Malaysia Pahang
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publishDate 2015
publisher Asia Pacific Publishing
recordtype eprints
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spelling ump-184472018-07-27T08:26:41Z http://umpir.ump.edu.my/id/eprint/18447/ Ultrasonic Assisted Extraction of Phenolic and Flavonoid Content from Orthosiphon Stamineus Leaves Sook, Fun Pang M. M., Yusoff Luqman Chuah, Abdullah Jolius, Gimbun TP Chemical technology This paper presents a combined effect of ultrasonic assisted extraction and solvents of varying polarities on phenolic compound extraction from O. stamineus. The polyphenol content in the plant extracts was analysed using Singleton’s method and aluminium chloride colorimetric assay. The results suggest that the polyphenol extraction from O. stamineus is affected by the solvent type. The highest phenolic content of 168.8 mg GAE/g DW was obtained from ultrasonic assisted extraction (UAE) using 70% aqueous methanol and 70% aqueous propanol solvent. The highest total flavonoid content of 185.3 mg QE/g DW was obtained using 70% aqueous propanol. The phenolic acid and flavonoid yield increased with extraction time, however, extraction beyond 120 min or at a temperature higher than 60°C induced degradation and hence reducing extraction yield. Asia Pacific Publishing 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/18447/1/70%20STP%201%2010-13.pdf Sook, Fun Pang and M. M., Yusoff and Luqman Chuah, Abdullah and Jolius, Gimbun (2015) Ultrasonic Assisted Extraction of Phenolic and Flavonoid Content from Orthosiphon Stamineus Leaves. Science Technology Progress, 1. pp. 10-13. ISSN 2454-5147. (Published) https://www.researchgate.net/publication/280426274_Ultrasonic_assisted_extraction_of_phenolic_and_flavonoid_content_from_Orthosiphon_stamineus_leaves
spellingShingle TP Chemical technology
Sook, Fun Pang
M. M., Yusoff
Luqman Chuah, Abdullah
Jolius, Gimbun
Ultrasonic Assisted Extraction of Phenolic and Flavonoid Content from Orthosiphon Stamineus Leaves
title Ultrasonic Assisted Extraction of Phenolic and Flavonoid Content from Orthosiphon Stamineus Leaves
title_full Ultrasonic Assisted Extraction of Phenolic and Flavonoid Content from Orthosiphon Stamineus Leaves
title_fullStr Ultrasonic Assisted Extraction of Phenolic and Flavonoid Content from Orthosiphon Stamineus Leaves
title_full_unstemmed Ultrasonic Assisted Extraction of Phenolic and Flavonoid Content from Orthosiphon Stamineus Leaves
title_short Ultrasonic Assisted Extraction of Phenolic and Flavonoid Content from Orthosiphon Stamineus Leaves
title_sort ultrasonic assisted extraction of phenolic and flavonoid content from orthosiphon stamineus leaves
topic TP Chemical technology
url http://umpir.ump.edu.my/id/eprint/18447/
http://umpir.ump.edu.my/id/eprint/18447/
http://umpir.ump.edu.my/id/eprint/18447/1/70%20STP%201%2010-13.pdf