Production of High Commercial Value Xylooligosaccharides from Meranti Wood Sawdust Using Immobilised Xylanase
The present study explores the utilisation of a new raw material from lignocellulose biomass, Meranti wood sawdust (MWS) for high commercial value xylooligosaccharides (XOS) production using immobilised xylanase. The xylanase was immobilised by a combination of entrapment and covalent binding techni...
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| Format: | Article |
| Language: | English English |
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Springer
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/18342/ http://umpir.ump.edu.my/id/eprint/18342/1/Production%20of%20High%20Commercial%20Value%20Xylooligosaccharides%20from%20Meranti%20Wood%20Sawdust%20Using%20Immobilised%20Xylanase.pdf http://umpir.ump.edu.my/id/eprint/18342/2/Production%20of%20High%20Commercial%20Value%20Xylooligosaccharides%20from%20Meranti%20Wood%20Sawdust%20Using%20Immobilised%20Xylanase%201.pdf |
| _version_ | 1848820649165848576 |
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| author | Siti Sabrina, Mohd Sukri Mimi Sakinah, A. M. |
| author_facet | Siti Sabrina, Mohd Sukri Mimi Sakinah, A. M. |
| author_sort | Siti Sabrina, Mohd Sukri |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The present study explores the utilisation of a new raw material from lignocellulose biomass, Meranti wood sawdust (MWS) for high commercial value xylooligosaccharides (XOS) production using immobilised xylanase. The xylanase was immobilised by a combination of entrapment and covalent binding techniques. The hemicellulosic xylan from MWS was extracted using a standard chlorite delignification method. The production of total and derivatives of XOS from the degradation of the hemicellulosic xylan of MWS were compared to the production from the commercial xylan from Beechwood. The utilisation of the extracted xylan from MWS yielded 0.36 mg/mL of total XOS after 60 h of hydrolysis. During the hydrolysis reaction, the immobilised xylanase released a lower degree of polymerisation (DP) of XOS, mainly X2 and X3, which were the major products of xylan degradation by xylanase enzymes. The production of XOS with a lower DP from MWS demonstrated the biotechnological potential of the MWS in the future. The XOS production retained about 70% of its initial XOS production during the second cycle. This is also the first report on the utilisation of MWS wastes in enzymatic hydrolysis using immobilised xylanase for XOS production. |
| first_indexed | 2025-11-15T02:12:48Z |
| format | Article |
| id | ump-18342 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T02:12:48Z |
| publishDate | 2017 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-183422017-08-10T03:01:43Z http://umpir.ump.edu.my/id/eprint/18342/ Production of High Commercial Value Xylooligosaccharides from Meranti Wood Sawdust Using Immobilised Xylanase Siti Sabrina, Mohd Sukri Mimi Sakinah, A. M. T Technology (General) TP Chemical technology The present study explores the utilisation of a new raw material from lignocellulose biomass, Meranti wood sawdust (MWS) for high commercial value xylooligosaccharides (XOS) production using immobilised xylanase. The xylanase was immobilised by a combination of entrapment and covalent binding techniques. The hemicellulosic xylan from MWS was extracted using a standard chlorite delignification method. The production of total and derivatives of XOS from the degradation of the hemicellulosic xylan of MWS were compared to the production from the commercial xylan from Beechwood. The utilisation of the extracted xylan from MWS yielded 0.36 mg/mL of total XOS after 60 h of hydrolysis. During the hydrolysis reaction, the immobilised xylanase released a lower degree of polymerisation (DP) of XOS, mainly X2 and X3, which were the major products of xylan degradation by xylanase enzymes. The production of XOS with a lower DP from MWS demonstrated the biotechnological potential of the MWS in the future. The XOS production retained about 70% of its initial XOS production during the second cycle. This is also the first report on the utilisation of MWS wastes in enzymatic hydrolysis using immobilised xylanase for XOS production. Springer 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/18342/1/Production%20of%20High%20Commercial%20Value%20Xylooligosaccharides%20from%20Meranti%20Wood%20Sawdust%20Using%20Immobilised%20Xylanase.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/18342/2/Production%20of%20High%20Commercial%20Value%20Xylooligosaccharides%20from%20Meranti%20Wood%20Sawdust%20Using%20Immobilised%20Xylanase%201.pdf Siti Sabrina, Mohd Sukri and Mimi Sakinah, A. M. (2017) Production of High Commercial Value Xylooligosaccharides from Meranti Wood Sawdust Using Immobilised Xylanase. Applied Biochemistry and Biotechnology. pp. 1-13. ISSN 1559-0291. (In Press / Online First) (In Press / Online First) https://link.springer.com/article/10.1007/s12010-017-2542-0 DOI: 10.1007/s12010-017-2542-0 |
| spellingShingle | T Technology (General) TP Chemical technology Siti Sabrina, Mohd Sukri Mimi Sakinah, A. M. Production of High Commercial Value Xylooligosaccharides from Meranti Wood Sawdust Using Immobilised Xylanase |
| title | Production of High Commercial Value Xylooligosaccharides from Meranti Wood Sawdust Using Immobilised Xylanase |
| title_full | Production of High Commercial Value Xylooligosaccharides from Meranti Wood Sawdust Using Immobilised Xylanase |
| title_fullStr | Production of High Commercial Value Xylooligosaccharides from Meranti Wood Sawdust Using Immobilised Xylanase |
| title_full_unstemmed | Production of High Commercial Value Xylooligosaccharides from Meranti Wood Sawdust Using Immobilised Xylanase |
| title_short | Production of High Commercial Value Xylooligosaccharides from Meranti Wood Sawdust Using Immobilised Xylanase |
| title_sort | production of high commercial value xylooligosaccharides from meranti wood sawdust using immobilised xylanase |
| topic | T Technology (General) TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/18342/ http://umpir.ump.edu.my/id/eprint/18342/ http://umpir.ump.edu.my/id/eprint/18342/ http://umpir.ump.edu.my/id/eprint/18342/1/Production%20of%20High%20Commercial%20Value%20Xylooligosaccharides%20from%20Meranti%20Wood%20Sawdust%20Using%20Immobilised%20Xylanase.pdf http://umpir.ump.edu.my/id/eprint/18342/2/Production%20of%20High%20Commercial%20Value%20Xylooligosaccharides%20from%20Meranti%20Wood%20Sawdust%20Using%20Immobilised%20Xylanase%201.pdf |