A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study
The direct electrochemistry of catalytically active cytochrome C (Cyt c) adsorbed together with a 3-dimensional network of chemically synthesized graphene on glassy carbon electrode has been readily obtained in aqueous phosphate buffer. Direct electrical communication between the redox center of Cyt...
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| Language: | English |
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Springer
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/18221/ http://umpir.ump.edu.my/id/eprint/18221/1/fist-2017-kafi-%20A%20Conductive%20Crosslinked%20Graphene1.pdf |
| _version_ | 1848820620875268096 |
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| author | A. K. M., Kafi M. M., Yusoff Choucair, Mohammad Crossley, Maxwell J. |
| author_facet | A. K. M., Kafi M. M., Yusoff Choucair, Mohammad Crossley, Maxwell J. |
| author_sort | A. K. M., Kafi |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The direct electrochemistry of catalytically active cytochrome C (Cyt c) adsorbed together with a 3-dimensional network of chemically synthesized graphene on glassy carbon electrode has been readily obtained in aqueous phosphate buffer. Direct electrical communication between the redox center of Cyt c and the modified graphene-based electrode was established. The modified electrode was employed as a high-performance hydrogen peroxide (H2O2) biosensor. The Cyt c present in modified electrode exhibited a pair of quasi-reversible redox peaks with a midpoint potential of −0.380 and −0.2 V, cathodic and anodic, respectively. Investigations into the electrocatalytic activity of the modified electrode upon hydrogen peroxide exposure revealed a rapid amperometric response (5 s). Under optimized conditions, the linear range of response to H2O2 concentration ranged from 5 × 10−7 to 2 × 10−4 M with a detection limit of 2 × 10−7 M at a signal-to-noise ratio of 3. The stability, reproducibility, and selectivity of the proposed biosensor are discussed in relation to the morphology and composition of the modified electrode. |
| first_indexed | 2025-11-15T02:12:21Z |
| format | Article |
| id | ump-18221 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:12:21Z |
| publishDate | 2017 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-182212018-07-26T03:20:24Z http://umpir.ump.edu.my/id/eprint/18221/ A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study A. K. M., Kafi M. M., Yusoff Choucair, Mohammad Crossley, Maxwell J. Q Science (General) The direct electrochemistry of catalytically active cytochrome C (Cyt c) adsorbed together with a 3-dimensional network of chemically synthesized graphene on glassy carbon electrode has been readily obtained in aqueous phosphate buffer. Direct electrical communication between the redox center of Cyt c and the modified graphene-based electrode was established. The modified electrode was employed as a high-performance hydrogen peroxide (H2O2) biosensor. The Cyt c present in modified electrode exhibited a pair of quasi-reversible redox peaks with a midpoint potential of −0.380 and −0.2 V, cathodic and anodic, respectively. Investigations into the electrocatalytic activity of the modified electrode upon hydrogen peroxide exposure revealed a rapid amperometric response (5 s). Under optimized conditions, the linear range of response to H2O2 concentration ranged from 5 × 10−7 to 2 × 10−4 M with a detection limit of 2 × 10−7 M at a signal-to-noise ratio of 3. The stability, reproducibility, and selectivity of the proposed biosensor are discussed in relation to the morphology and composition of the modified electrode. Springer 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/18221/1/fist-2017-kafi-%20A%20Conductive%20Crosslinked%20Graphene1.pdf A. K. M., Kafi and M. M., Yusoff and Choucair, Mohammad and Crossley, Maxwell J. (2017) A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study. Journal of Solid State Electrochemistry, 21 (10). pp. 2761-2767. ISSN 1433-0768. (Published) https://doi.org/10.1007/s10008-017-3598-z doi: 10.1007/s10008-017-3598-z |
| spellingShingle | Q Science (General) A. K. M., Kafi M. M., Yusoff Choucair, Mohammad Crossley, Maxwell J. A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study |
| title | A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study |
| title_full | A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study |
| title_fullStr | A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study |
| title_full_unstemmed | A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study |
| title_short | A Conductive Crosslinked Graphene/Cytochrome C Networks for The Electrochemical and Biosensing Study |
| title_sort | conductive crosslinked graphene/cytochrome c networks for the electrochemical and biosensing study |
| topic | Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/18221/ http://umpir.ump.edu.my/id/eprint/18221/ http://umpir.ump.edu.my/id/eprint/18221/ http://umpir.ump.edu.my/id/eprint/18221/1/fist-2017-kafi-%20A%20Conductive%20Crosslinked%20Graphene1.pdf |