Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors
In order to maximize enzymatic xylan depolymerization while simultaneously purifying the resulting monosaccharide (xylose), different ultrafiltration (UF) membrane reactor configurations were evaluated. Initial results showed that the two hydrolytic enzymes required for complete depolymerization of...
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Elsevier Ltd
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/16536/ http://umpir.ump.edu.my/id/eprint/16536/1/Impact%20of%20the%20fouling%20mechanism%20on%20enzymatic%20depolymerization%20of%20xylan%20in%20different%20configurations%20of%20membrane%20reactors.pdf |
| _version_ | 1848820215387783168 |
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| author | Mohd Shafiq, Mohd Sueb Luo, Jianquan Meyer, Anne S. Jørgensen, Henning Pinelo, Manuel |
| author_facet | Mohd Shafiq, Mohd Sueb Luo, Jianquan Meyer, Anne S. Jørgensen, Henning Pinelo, Manuel |
| author_sort | Mohd Shafiq, Mohd Sueb |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | In order to maximize enzymatic xylan depolymerization while simultaneously purifying the resulting monosaccharide (xylose), different ultrafiltration (UF) membrane reactor configurations were evaluated. Initial results showed that the two hydrolytic enzymes required for complete depolymerization of xylan, endo-1,4-β-xylanase and β-xylosidase, promoted different types of fouling, which had a direct impact on the extent of xylan hydrolysis achieved during reaction. Endo-1,4-β-xylanase generated DP 1–6 xylo-oligomers. These products contributed to partial pore blocking of the 1 kDa polysulfone membrane and caused irreversible flux loss (∼20%). The presence of β-xylosidase could not prevent deposition of xylan and xylooligomers on the UF membrane surface. Mulder’s modelling of the filtration parameters affirmed that this xylan and xylooligosaccharide deposition formed a cake layer on the membrane which hindered enzymatic attack in addition to fouling. Reaction with both enzymes followed by UF was found to be the optimal configuration, providing at least 40% higher xylan hydrolysis than the cascade configuration (involving sequential reaction with each of the enzymes separately) and the simultaneous reaction-filtration with both enzymes, respectively. This study thus confirmed that the reactor configuration has a crucial impact on the performance of both the reaction and the separation process of xylose during enzymatic xylan degradation, and that the type of fouling mechanism varies in response to the type of enzyme treatment. |
| first_indexed | 2025-11-15T02:05:55Z |
| format | Article |
| id | ump-16536 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:05:55Z |
| publishDate | 2017 |
| publisher | Elsevier Ltd |
| recordtype | eprints |
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| spelling | ump-165362017-04-25T07:28:25Z http://umpir.ump.edu.my/id/eprint/16536/ Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors Mohd Shafiq, Mohd Sueb Luo, Jianquan Meyer, Anne S. Jørgensen, Henning Pinelo, Manuel TP Chemical technology In order to maximize enzymatic xylan depolymerization while simultaneously purifying the resulting monosaccharide (xylose), different ultrafiltration (UF) membrane reactor configurations were evaluated. Initial results showed that the two hydrolytic enzymes required for complete depolymerization of xylan, endo-1,4-β-xylanase and β-xylosidase, promoted different types of fouling, which had a direct impact on the extent of xylan hydrolysis achieved during reaction. Endo-1,4-β-xylanase generated DP 1–6 xylo-oligomers. These products contributed to partial pore blocking of the 1 kDa polysulfone membrane and caused irreversible flux loss (∼20%). The presence of β-xylosidase could not prevent deposition of xylan and xylooligomers on the UF membrane surface. Mulder’s modelling of the filtration parameters affirmed that this xylan and xylooligosaccharide deposition formed a cake layer on the membrane which hindered enzymatic attack in addition to fouling. Reaction with both enzymes followed by UF was found to be the optimal configuration, providing at least 40% higher xylan hydrolysis than the cascade configuration (involving sequential reaction with each of the enzymes separately) and the simultaneous reaction-filtration with both enzymes, respectively. This study thus confirmed that the reactor configuration has a crucial impact on the performance of both the reaction and the separation process of xylose during enzymatic xylan degradation, and that the type of fouling mechanism varies in response to the type of enzyme treatment. Elsevier Ltd 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16536/1/Impact%20of%20the%20fouling%20mechanism%20on%20enzymatic%20depolymerization%20of%20xylan%20in%20different%20configurations%20of%20membrane%20reactors.pdf Mohd Shafiq, Mohd Sueb and Luo, Jianquan and Meyer, Anne S. and Jørgensen, Henning and Pinelo, Manuel (2017) Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors. Separation and Purification Technology, 178. pp. 154-162. ISSN 1383-5866. (Published) http://doi.org/10.1016/j.seppur.2017.01.038 DOI: 10.1016/j.seppur.2017.01.038 |
| spellingShingle | TP Chemical technology Mohd Shafiq, Mohd Sueb Luo, Jianquan Meyer, Anne S. Jørgensen, Henning Pinelo, Manuel Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors |
| title | Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors |
| title_full | Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors |
| title_fullStr | Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors |
| title_full_unstemmed | Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors |
| title_short | Impact of the Fouling Mechanism on Enzymatic Depolymerization of Xylan in Different Configurations of Membrane Reactors |
| title_sort | impact of the fouling mechanism on enzymatic depolymerization of xylan in different configurations of membrane reactors |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/16536/ http://umpir.ump.edu.my/id/eprint/16536/ http://umpir.ump.edu.my/id/eprint/16536/ http://umpir.ump.edu.my/id/eprint/16536/1/Impact%20of%20the%20fouling%20mechanism%20on%20enzymatic%20depolymerization%20of%20xylan%20in%20different%20configurations%20of%20membrane%20reactors.pdf |