Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations
Continuous removal of ethanol during production by the anaerobic bacterium Zymomonas mobilis mitigated ethanol inhibition. Ethanol inhibited its own production at a concentration of >20 g L−1. Ethanol productivity was enhanced by continuous in situ extraction with water-immiscible organic solvent...
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Elsevier Ltd
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/16450/ |
| _version_ | 1848820196193599488 |
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| author | Azilah, Ajit Ahmad Ziad, Sulaiman Chisti, Yusuf |
| author_facet | Azilah, Ajit Ahmad Ziad, Sulaiman Chisti, Yusuf |
| author_sort | Azilah, Ajit |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Continuous removal of ethanol during production by the anaerobic bacterium Zymomonas mobilis mitigated ethanol inhibition. Ethanol inhibited its own production at a concentration of >20 g L−1. Ethanol productivity was enhanced by continuous in situ extraction with water-immiscible organic solvents. Several solvents were evaluated for biocompatibility with Z. mobilis and the ability to extract ethanol from a batch fermentation. In situ batch extractive fermentations were carried out in a 2-L stirred bioreactor (1:1 volume ratio of solvent and fermentation broth) with an optimal glucose concentration of 150 g L−1, 35 °C, and an agitation speed of 150 rpm. Oleyl alcohol, iso-octadecanol and 2-octyl-1-dodecanol were the three solvents that were most compatible with Z. mobilis. With these solvents, the cell viability relative to control (no solvent) was 1.48 ± 0.40, 1.03 ± 0.18 and 1.05 ± 0.07, respectively, after two days of exposure. All solvents tested improved the final biomass concentration, the glucose consumption and ethanol production relative to control, but iso-octadecanol was clearly the most effective solvent. Using iso-octadecanol, the maximum ethanol concentration of 75 ± 3 g L−1 was attained. This was nearly 1.25-fold that of the control fermentation. For this solvent, the ethanol yield on glucose was 0.485 ± 0.005 g g−1 compared to a yield of 0.468 ± 0.005 g g−1 for the control culture. |
| first_indexed | 2025-11-15T02:05:36Z |
| format | Article |
| id | ump-16450 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| last_indexed | 2025-11-15T02:05:36Z |
| publishDate | 2017 |
| publisher | Elsevier Ltd |
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| spelling | ump-164502018-01-30T02:58:34Z http://umpir.ump.edu.my/id/eprint/16450/ Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations Azilah, Ajit Ahmad Ziad, Sulaiman Chisti, Yusuf TP Chemical technology Continuous removal of ethanol during production by the anaerobic bacterium Zymomonas mobilis mitigated ethanol inhibition. Ethanol inhibited its own production at a concentration of >20 g L−1. Ethanol productivity was enhanced by continuous in situ extraction with water-immiscible organic solvents. Several solvents were evaluated for biocompatibility with Z. mobilis and the ability to extract ethanol from a batch fermentation. In situ batch extractive fermentations were carried out in a 2-L stirred bioreactor (1:1 volume ratio of solvent and fermentation broth) with an optimal glucose concentration of 150 g L−1, 35 °C, and an agitation speed of 150 rpm. Oleyl alcohol, iso-octadecanol and 2-octyl-1-dodecanol were the three solvents that were most compatible with Z. mobilis. With these solvents, the cell viability relative to control (no solvent) was 1.48 ± 0.40, 1.03 ± 0.18 and 1.05 ± 0.07, respectively, after two days of exposure. All solvents tested improved the final biomass concentration, the glucose consumption and ethanol production relative to control, but iso-octadecanol was clearly the most effective solvent. Using iso-octadecanol, the maximum ethanol concentration of 75 ± 3 g L−1 was attained. This was nearly 1.25-fold that of the control fermentation. For this solvent, the ethanol yield on glucose was 0.485 ± 0.005 g g−1 compared to a yield of 0.468 ± 0.005 g g−1 for the control culture. Elsevier Ltd 2017 Article PeerReviewed Azilah, Ajit and Ahmad Ziad, Sulaiman and Chisti, Yusuf (2017) Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations. Food and Bioproducts Processing, 102. pp. 123-135. ISSN 0960-3085. (Published) http://dx.doi.org/10.1016/j.fbp.2016.12.006 DOI: 10.1016/j.fbp.2016.12.006 |
| spellingShingle | TP Chemical technology Azilah, Ajit Ahmad Ziad, Sulaiman Chisti, Yusuf Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations |
| title | Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations |
| title_full | Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations |
| title_fullStr | Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations |
| title_full_unstemmed | Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations |
| title_short | Production of Bioethanol by Zymomonas Mobilis in High-gravity Extractive Fermentations |
| title_sort | production of bioethanol by zymomonas mobilis in high-gravity extractive fermentations |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/16450/ http://umpir.ump.edu.my/id/eprint/16450/ http://umpir.ump.edu.my/id/eprint/16450/ |