Bioassay-guided Isolation and Antioxidant Activity of Sulfur-containing Compounds from Clinacanthus nutans
Clinacanthus nutans has been used in traditional herbal medicine for cancer prevention, but the specific bioactive compounds responsible for the observed activities have not been explored. Different polar solvents such as methanol, chloroform, ethyl acetate, and hexane were used for the extraction....
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| Language: | English |
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Wiley
2016
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| Online Access: | http://umpir.ump.edu.my/id/eprint/16242/ http://umpir.ump.edu.my/id/eprint/16242/6/fist-2016-hazrul-bioassay.pdf |
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| author | Hazrulrizawati, Hamid Izzah Hayati, Yahaya M. M., Yusoff Seema, Zareen |
| author_facet | Hazrulrizawati, Hamid Izzah Hayati, Yahaya M. M., Yusoff Seema, Zareen |
| author_sort | Hazrulrizawati, Hamid |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Clinacanthus nutans has been used in traditional herbal medicine for cancer prevention, but the specific bioactive compounds responsible for the observed activities have not been explored. Different polar solvents such as methanol, chloroform, ethyl acetate, and hexane were used for the extraction. The extracts, fractions, and isolated compounds were subjected to DPPH and ferric reducing antioxidant potential (FRAP) assays. Methanol extracts show significant free-radical scavenging activity of 69.09% in DPPH and 56.49% FRAP. Purification of MeOH extracts afforded the fraction FB28 and two new sulfur-containing compounds, named clinamide D and E (1, 2). Compound (1) proved to be more active with an IC50 value for DPPH radical scavenging of 118.27 ± 0.01 µg/mL and reduction of Fe3+–TPTZ complex of 386.24 ± 0.02, higher than that of the standard ascorbic acid. Sulfur-containing compounds isolated from C. nutans is a potential natural antioxidant. |
| first_indexed | 2025-11-15T02:04:50Z |
| format | Article |
| id | ump-16242 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:04:50Z |
| publishDate | 2016 |
| publisher | Wiley |
| recordtype | eprints |
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| spelling | ump-162422018-07-26T07:50:33Z http://umpir.ump.edu.my/id/eprint/16242/ Bioassay-guided Isolation and Antioxidant Activity of Sulfur-containing Compounds from Clinacanthus nutans Hazrulrizawati, Hamid Izzah Hayati, Yahaya M. M., Yusoff Seema, Zareen QD Chemistry Clinacanthus nutans has been used in traditional herbal medicine for cancer prevention, but the specific bioactive compounds responsible for the observed activities have not been explored. Different polar solvents such as methanol, chloroform, ethyl acetate, and hexane were used for the extraction. The extracts, fractions, and isolated compounds were subjected to DPPH and ferric reducing antioxidant potential (FRAP) assays. Methanol extracts show significant free-radical scavenging activity of 69.09% in DPPH and 56.49% FRAP. Purification of MeOH extracts afforded the fraction FB28 and two new sulfur-containing compounds, named clinamide D and E (1, 2). Compound (1) proved to be more active with an IC50 value for DPPH radical scavenging of 118.27 ± 0.01 µg/mL and reduction of Fe3+–TPTZ complex of 386.24 ± 0.02, higher than that of the standard ascorbic acid. Sulfur-containing compounds isolated from C. nutans is a potential natural antioxidant. Wiley 2016-11-16 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/16242/6/fist-2016-hazrul-bioassay.pdf Hazrulrizawati, Hamid and Izzah Hayati, Yahaya and M. M., Yusoff and Seema, Zareen (2016) Bioassay-guided Isolation and Antioxidant Activity of Sulfur-containing Compounds from Clinacanthus nutans. Journal of the Chinese Chemical Society, 63 (12). pp. 1033-1037. ISSN 2192-6549. (Published) http://dx.doi.org/10.1002/jccs.201600755 |
| spellingShingle | QD Chemistry Hazrulrizawati, Hamid Izzah Hayati, Yahaya M. M., Yusoff Seema, Zareen Bioassay-guided Isolation and Antioxidant Activity of Sulfur-containing Compounds from Clinacanthus nutans |
| title | Bioassay-guided Isolation and Antioxidant Activity of Sulfur-containing Compounds from Clinacanthus nutans
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| title_full | Bioassay-guided Isolation and Antioxidant Activity of Sulfur-containing Compounds from Clinacanthus nutans
|
| title_fullStr | Bioassay-guided Isolation and Antioxidant Activity of Sulfur-containing Compounds from Clinacanthus nutans
|
| title_full_unstemmed | Bioassay-guided Isolation and Antioxidant Activity of Sulfur-containing Compounds from Clinacanthus nutans
|
| title_short | Bioassay-guided Isolation and Antioxidant Activity of Sulfur-containing Compounds from Clinacanthus nutans
|
| title_sort | bioassay-guided isolation and antioxidant activity of sulfur-containing compounds from clinacanthus nutans |
| topic | QD Chemistry |
| url | http://umpir.ump.edu.my/id/eprint/16242/ http://umpir.ump.edu.my/id/eprint/16242/ http://umpir.ump.edu.my/id/eprint/16242/6/fist-2016-hazrul-bioassay.pdf |