Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks
We report on a novel 3-dimensional (3D) network of crosslinked Cytochrome C/Carbon Nanotube (CytC/CNT) on a thiol-modified gold surface which can establish direct electrical communication between the redox center of Cytochrome C and the electrode. Cyclic voltammograms (CVs) results showed a pair of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Scientific Publishers
2017
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| Online Access: | http://umpir.ump.edu.my/id/eprint/15616/ http://umpir.ump.edu.my/id/eprint/15616/1/fist-2017-kafi-Superoxide%20Radical%20Biosensor%20Based1.pdf |
| _version_ | 1848819999115837440 |
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| author | A. K. M., Kafi N. S., Azmi M. M., Yusoff Crossley, Maxwell J. |
| author_facet | A. K. M., Kafi N. S., Azmi M. M., Yusoff Crossley, Maxwell J. |
| author_sort | A. K. M., Kafi |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | We report on a novel 3-dimensional (3D) network of crosslinked Cytochrome C/Carbon Nanotube (CytC/CNT) on a thiol-modified gold surface which can establish direct electrical communication between the redox center of Cytochrome C and the electrode. Cyclic voltammograms (CVs) results showed a pair of well defined redox peaks for Cytochrome C, located at about −0.03 and +0.06 V,
cathodic and anodic respectively. Additionally, the formal potential E0 of adsorbed Cyt c was found to be 15 mV, a value close to that of native Cyt c. Based on 3D Cytochrome c and carbon nanotube network, a sensitive superoxide radical biosensor has been proposed. The biosensor showed high sensitivity and lower detection limit of 0.3 �M of superoxide. |
| first_indexed | 2025-11-15T02:02:28Z |
| format | Article |
| id | ump-15616 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:02:28Z |
| publishDate | 2017 |
| publisher | American Scientific Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-156162018-07-26T03:23:49Z http://umpir.ump.edu.my/id/eprint/15616/ Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks A. K. M., Kafi N. S., Azmi M. M., Yusoff Crossley, Maxwell J. QD Chemistry We report on a novel 3-dimensional (3D) network of crosslinked Cytochrome C/Carbon Nanotube (CytC/CNT) on a thiol-modified gold surface which can establish direct electrical communication between the redox center of Cytochrome C and the electrode. Cyclic voltammograms (CVs) results showed a pair of well defined redox peaks for Cytochrome C, located at about −0.03 and +0.06 V, cathodic and anodic respectively. Additionally, the formal potential E0 of adsorbed Cyt c was found to be 15 mV, a value close to that of native Cyt c. Based on 3D Cytochrome c and carbon nanotube network, a sensitive superoxide radical biosensor has been proposed. The biosensor showed high sensitivity and lower detection limit of 0.3 �M of superoxide. American Scientific Publishers 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/15616/1/fist-2017-kafi-Superoxide%20Radical%20Biosensor%20Based1.pdf A. K. M., Kafi and N. S., Azmi and M. M., Yusoff and Crossley, Maxwell J. (2017) Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks. Journal of Nanoscience and Nanotechnology, 17 (8). pp. 5896-5899. ISSN 1533-4880 (Print); 1533-4899 (Online). (Published) http://www.ingentaconnect.com/content/asp/jnn/2017/00000017/00000008/art00128 doi: 10.1166/jnn.2017.13860 |
| spellingShingle | QD Chemistry A. K. M., Kafi N. S., Azmi M. M., Yusoff Crossley, Maxwell J. Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks |
| title | Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks |
| title_full | Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks |
| title_fullStr | Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks |
| title_full_unstemmed | Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks |
| title_short | Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks |
| title_sort | superoxide radical biosensor based on a 3d enzyme/carbon nanotube conductive networks |
| topic | QD Chemistry |
| url | http://umpir.ump.edu.my/id/eprint/15616/ http://umpir.ump.edu.my/id/eprint/15616/ http://umpir.ump.edu.my/id/eprint/15616/ http://umpir.ump.edu.my/id/eprint/15616/1/fist-2017-kafi-Superoxide%20Radical%20Biosensor%20Based1.pdf |