Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3)
Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into th...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Universiti Malaysia Pahang
2016
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/15042/ http://umpir.ump.edu.my/id/eprint/15042/1/P011%20pg71-76.pdf |
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| author | Wan Siti Atikah, Wan Omar Ahmad Ziad, Sulaiman Azilah, Ajit Yusuf, Chisti Mohd Hairul, Ab Rahim Adam Leow, Thean Chor |
| author_facet | Wan Siti Atikah, Wan Omar Ahmad Ziad, Sulaiman Azilah, Ajit Yusuf, Chisti Mohd Hairul, Ab Rahim Adam Leow, Thean Chor |
| author_sort | Wan Siti Atikah, Wan Omar |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation. |
| first_indexed | 2025-11-15T02:00:13Z |
| format | Conference or Workshop Item |
| id | ump-15042 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T02:00:13Z |
| publishDate | 2016 |
| publisher | Universiti Malaysia Pahang |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-150422020-06-09T03:15:59Z http://umpir.ump.edu.my/id/eprint/15042/ Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) Wan Siti Atikah, Wan Omar Ahmad Ziad, Sulaiman Azilah, Ajit Yusuf, Chisti Mohd Hairul, Ab Rahim Adam Leow, Thean Chor TP Chemical technology Recombinant microorganism is a genetically modified microorganism which specifically designed for the expression of target protein production or bioreaction process. As different expression host may contribute to superior results, the transformation of a plasmid carrying the gene of interest into the same strain of competent cells would assist in research assessment. This study focused on the transformation of recombinant lipase from two different sources into Escherichia coli competent cells, BL21 (DE3). The recombinant cells, pUC8.2-14 (American Type Culture Collection (ATCC)®68046™) was harbouring Rhizopus (delemar) oryzae lipase and E. coli Top 10 pTrcHis was harbouring Staphylococcus hyicus lipase, respectively. The transformation is involved preparation of competent cells, polymerase chain reaction (PCR) and DNA sequencing. The DNA sequences obtained was inserted into Basic Local Alignment Search Tool (BLAST), under the databases of National Centre for Biotechnology Information (NCBI), USA. The findings show the respective plasmids were successfully carried by the E. coli BL21 (DE3) for further investigation. Universiti Malaysia Pahang 2016 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/15042/1/P011%20pg71-76.pdf Wan Siti Atikah, Wan Omar and Ahmad Ziad, Sulaiman and Azilah, Ajit and Yusuf, Chisti and Mohd Hairul, Ab Rahim and Adam Leow, Thean Chor (2016) Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3). In: Proceedings of The National Conference for Postgraduate Research (NCON-PGR 2016) , 24-25 September 2016 , Universiti Malaysia Pahang (UMP), Pekan, Pahang. pp. 71-76.. (Published) http://ee.ump.edu.my/ncon/wp-content/uploads/2016/10/Proceeding-NCON-PGR-2016.zip |
| spellingShingle | TP Chemical technology Wan Siti Atikah, Wan Omar Ahmad Ziad, Sulaiman Azilah, Ajit Yusuf, Chisti Mohd Hairul, Ab Rahim Adam Leow, Thean Chor Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title | Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title_full | Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title_fullStr | Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title_full_unstemmed | Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title_short | Transformation of pTrcHis and pUC8.2-14 into Escherichia coli BL21 (DE3) |
| title_sort | transformation of ptrchis and puc8.2-14 into escherichia coli bl21 (de3) |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/15042/ http://umpir.ump.edu.my/id/eprint/15042/ http://umpir.ump.edu.my/id/eprint/15042/1/P011%20pg71-76.pdf |