In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves
The present study aimed to study the anti-oxidant potential of isolated flavonoids from the ethanol extract (70%v/v) of Cassia tora leaves. Three flavonoids were isolated viz Luteolin-7-O-β-glucopyranoside (I), quercetin-3-O-β-d-glucuronide (II) and Formononetin-7-O-β-D-Glucoside (III) from the etha...
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| Format: | Article |
| Language: | English English |
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Penerbit Universiti Malaysia Pahang
2016
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| Online Access: | http://umpir.ump.edu.my/id/eprint/13806/ http://umpir.ump.edu.my/id/eprint/13806/1/In-vitro%20antioxidant%20potential%20of%20the%20flavonoid%20glycosides%20from%20Cassia%20tora%20linn.%20Leaves.pdf http://umpir.ump.edu.my/id/eprint/13806/7/ftech-2016-masilamani-Invitro%20antioxidant%20potential1.pdf |
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| author | Vijayalakshmi, A. Jayakumari, S. Masilamani, K. |
| author_facet | Vijayalakshmi, A. Jayakumari, S. Masilamani, K. |
| author_sort | Vijayalakshmi, A. |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The present study aimed to study the anti-oxidant potential of isolated flavonoids from the ethanol extract (70%v/v) of Cassia tora leaves. Three flavonoids were isolated viz Luteolin-7-O-β-glucopyranoside (I), quercetin-3-O-β-d-glucuronide (II) and Formononetin-7-O-β-D-Glucoside (III) from the ethanol extract (70%v/v) of Cassia tora leaves in the previous study. A comprehensive study on the phytochemical contents - total phenolic content and flavonoid content was accessed using Folin-Denis and AlCl3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and nitric oxide scavenging methods. The total phenolic content and flavonoid content was found to be 18.60 % w/w and 9.5% w/w respectively. The IC50 values of ethanol extract against hydroxyl, DPPH and nitric oxide radical were found to be 270 µg/mL, 190 µg/mL and 130 µg/mL respectively. The radical
scavenging activity of the isolated flavonoids decreased in the following order: quercetin (IC50 values 15, 14, 18
µg/mL) >formononetin (IC50 values 19, 21, 14 µg/mL) > luteolin (IC50 values 20, 23, 18 µg/mL) respectively.
Index terms - Cassia tora, flavonoids, HPTLC, antioxidant |
| first_indexed | 2025-11-15T01:55:34Z |
| format | Article |
| id | ump-13806 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T01:55:34Z |
| publishDate | 2016 |
| publisher | Penerbit Universiti Malaysia Pahang |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-138062018-02-01T06:47:13Z http://umpir.ump.edu.my/id/eprint/13806/ In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves Vijayalakshmi, A. Jayakumari, S. Masilamani, K. T Technology (General) The present study aimed to study the anti-oxidant potential of isolated flavonoids from the ethanol extract (70%v/v) of Cassia tora leaves. Three flavonoids were isolated viz Luteolin-7-O-β-glucopyranoside (I), quercetin-3-O-β-d-glucuronide (II) and Formononetin-7-O-β-D-Glucoside (III) from the ethanol extract (70%v/v) of Cassia tora leaves in the previous study. A comprehensive study on the phytochemical contents - total phenolic content and flavonoid content was accessed using Folin-Denis and AlCl3 method respectively. The antioxidant potential of the samples was evaluated using inhibition of hydroxyl radical, 2,2-diphenyl-1- picrylhydrazyl (DPPH), and nitric oxide scavenging methods. The total phenolic content and flavonoid content was found to be 18.60 % w/w and 9.5% w/w respectively. The IC50 values of ethanol extract against hydroxyl, DPPH and nitric oxide radical were found to be 270 µg/mL, 190 µg/mL and 130 µg/mL respectively. The radical scavenging activity of the isolated flavonoids decreased in the following order: quercetin (IC50 values 15, 14, 18 µg/mL) >formononetin (IC50 values 19, 21, 14 µg/mL) > luteolin (IC50 values 20, 23, 18 µg/mL) respectively. Index terms - Cassia tora, flavonoids, HPTLC, antioxidant Penerbit Universiti Malaysia Pahang 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/13806/1/In-vitro%20antioxidant%20potential%20of%20the%20flavonoid%20glycosides%20from%20Cassia%20tora%20linn.%20Leaves.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/13806/7/ftech-2016-masilamani-Invitro%20antioxidant%20potential1.pdf Vijayalakshmi, A. and Jayakumari, S. and Masilamani, K. (2016) In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves. International Journal of Engineering Technology And Sciences (IJETS), 5 (1). pp. 19-26. ISSN 2289-697X. (Published) http://ijets.ump.edu.my/images/archive/Vol5/IJETS3.pdf DOI: 10.15282/ijets.5.2016.1.3.1042 |
| spellingShingle | T Technology (General) Vijayalakshmi, A. Jayakumari, S. Masilamani, K. In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves |
| title | In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves |
| title_full | In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves |
| title_fullStr | In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves |
| title_full_unstemmed | In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves |
| title_short | In-vitro Antioxidant Potential of the Flavonoid Glycosides from Cassia tora linn. Leaves |
| title_sort | in-vitro antioxidant potential of the flavonoid glycosides from cassia tora linn. leaves |
| topic | T Technology (General) |
| url | http://umpir.ump.edu.my/id/eprint/13806/ http://umpir.ump.edu.my/id/eprint/13806/ http://umpir.ump.edu.my/id/eprint/13806/ http://umpir.ump.edu.my/id/eprint/13806/1/In-vitro%20antioxidant%20potential%20of%20the%20flavonoid%20glycosides%20from%20Cassia%20tora%20linn.%20Leaves.pdf http://umpir.ump.edu.my/id/eprint/13806/7/ftech-2016-masilamani-Invitro%20antioxidant%20potential1.pdf |