Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures
The present study aimed to investigate the effect of different culture conditions on biomass content and flavonoid production of the elite C. asiatica accession UPM03. When 0.1 g inoculum 25 mL-1 of cell was grown in Murashige & Skoog (MS) medium supplemented with 2 mg 2,4-D l-1 and 1 mg kinetin...
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| Format: | Article |
| Language: | English |
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eSAT Publishing House Pvt. Ltd.
2013
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| Online Access: | http://umpir.ump.edu.my/id/eprint/12672/ http://umpir.ump.edu.my/id/eprint/12672/1/IJRET_110210037%202013.pdf |
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| author | Tan, Suat Hian Maziah, Mahmood Ariff, Arbakariya |
| author_facet | Tan, Suat Hian Maziah, Mahmood Ariff, Arbakariya |
| author_sort | Tan, Suat Hian |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | The present study aimed to investigate the effect of different culture conditions on biomass content and flavonoid production of the elite C. asiatica accession UPM03. When 0.1 g inoculum 25 mL-1 of cell was grown in Murashige & Skoog (MS) medium supplemented with 2 mg 2,4-D l-1 and 1 mg kinetin l-1, flavonoids were not significantly produced in cells or released into the culture medium. Production of flavonoid was correlated with the aggregation size and inoculum density. At aggregate size of 250-500 μm with 0.3 g inoculum density 25 mL-1, it gave the highest luteolin content with 35.45 μg g-1 DW. After investigating the effect of culture conditions, i.e. pH, inoculum density, light irradiation and plant growth regulator, we found that with the optimized condition (i.e.250-500 μm aggregate size, 0.5 g wet cell 25 mL-1 supplemented with 3 mg L-1 2,4-D and 1 mg L-1 kinetin at pH 5.7 under 16hphotoperiod) the luteolin content was 11-fold higher than the cell suspension cultured at an inoculum size of 0.3 g wet cell 25 mL-1 with 250-500 μm aggregate size. |
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| format | Article |
| id | ump-12672 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:51:14Z |
| publishDate | 2013 |
| publisher | eSAT Publishing House Pvt. Ltd. |
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| spelling | ump-126722018-06-29T08:45:31Z http://umpir.ump.edu.my/id/eprint/12672/ Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures Tan, Suat Hian Maziah, Mahmood Ariff, Arbakariya SB Plant culture The present study aimed to investigate the effect of different culture conditions on biomass content and flavonoid production of the elite C. asiatica accession UPM03. When 0.1 g inoculum 25 mL-1 of cell was grown in Murashige & Skoog (MS) medium supplemented with 2 mg 2,4-D l-1 and 1 mg kinetin l-1, flavonoids were not significantly produced in cells or released into the culture medium. Production of flavonoid was correlated with the aggregation size and inoculum density. At aggregate size of 250-500 μm with 0.3 g inoculum density 25 mL-1, it gave the highest luteolin content with 35.45 μg g-1 DW. After investigating the effect of culture conditions, i.e. pH, inoculum density, light irradiation and plant growth regulator, we found that with the optimized condition (i.e.250-500 μm aggregate size, 0.5 g wet cell 25 mL-1 supplemented with 3 mg L-1 2,4-D and 1 mg L-1 kinetin at pH 5.7 under 16hphotoperiod) the luteolin content was 11-fold higher than the cell suspension cultured at an inoculum size of 0.3 g wet cell 25 mL-1 with 250-500 μm aggregate size. eSAT Publishing House Pvt. Ltd. 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12672/1/IJRET_110210037%202013.pdf Tan, Suat Hian and Maziah, Mahmood and Ariff, Arbakariya (2013) Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures. International Journal of Research in Engineering and Technology (IJRET), 2 (8). pp. 244-253. ISSN 2321-7308 (print); 2319-1163 (online). (Published) http://esatjournals.net/ijret/2013v02/i10/IJRET20130210037.pdf |
| spellingShingle | SB Plant culture Tan, Suat Hian Maziah, Mahmood Ariff, Arbakariya Synergism Effect Between Inoculum Size and Aggregate Size on Flavonoid Production In Centella Asiatica (L.) Urban (Pegaga) Cell Suspension Cultures |
| title | Synergism Effect Between Inoculum Size and Aggregate
Size on Flavonoid Production In Centella Asiatica (L.)
Urban (Pegaga) Cell Suspension Cultures |
| title_full | Synergism Effect Between Inoculum Size and Aggregate
Size on Flavonoid Production In Centella Asiatica (L.)
Urban (Pegaga) Cell Suspension Cultures |
| title_fullStr | Synergism Effect Between Inoculum Size and Aggregate
Size on Flavonoid Production In Centella Asiatica (L.)
Urban (Pegaga) Cell Suspension Cultures |
| title_full_unstemmed | Synergism Effect Between Inoculum Size and Aggregate
Size on Flavonoid Production In Centella Asiatica (L.)
Urban (Pegaga) Cell Suspension Cultures |
| title_short | Synergism Effect Between Inoculum Size and Aggregate
Size on Flavonoid Production In Centella Asiatica (L.)
Urban (Pegaga) Cell Suspension Cultures |
| title_sort | synergism effect between inoculum size and aggregate
size on flavonoid production in centella asiatica (l.)
urban (pegaga) cell suspension cultures |
| topic | SB Plant culture |
| url | http://umpir.ump.edu.my/id/eprint/12672/ http://umpir.ump.edu.my/id/eprint/12672/ http://umpir.ump.edu.my/id/eprint/12672/1/IJRET_110210037%202013.pdf |