Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography
Membrane chromatography has been used widely as an alternative to the conventional packed bed chromatography for protein separation. Membrane chromatography used an adsorptive membrane that carried specific chromatography functionality. In the current study, a membrane chromatography was prepared by...
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| Format: | Article |
| Language: | English |
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Asian J. Chem
2016
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| Online Access: | http://umpir.ump.edu.my/id/eprint/12010/ http://umpir.ump.edu.my/id/eprint/12010/1/Comparison%20of%20Chemical%20and%20UV%20Photo-Grafting%20Modification%20on%20Polyamide.pdf |
| _version_ | 1848819127493328896 |
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| author | Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Nurul Izwanie, Rasli |
| author_facet | Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Nurul Izwanie, Rasli |
| author_sort | Syed Mohd Saufi, Tuan Chik |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Membrane chromatography has been used widely as an alternative to the conventional packed bed chromatography for protein separation. Membrane chromatography used an adsorptive membrane that carried specific chromatography functionality. In the current study, a membrane chromatography was prepared by modification of commercial polyamide microfiltration membrane with acrylic acid monomer. Two modification methods were compared which are UV photo grafting and chemical grafting via redox reaction. Modification parameters studied were initiator concentration (1-50 mM), monomer concentration (0.2-5 M) and reaction time (5-60 min). The highest lysozyme binding capacity achieved was 0.175 mg of lysozyme/cm2 membrane for the membrane prepared via UV photo grafting using 10 mM of
photo-initiator, 0.1 M of acrylic acid and 15 min of reaction time. |
| first_indexed | 2025-11-15T01:48:37Z |
| format | Article |
| id | ump-12010 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:48:37Z |
| publishDate | 2016 |
| publisher | Asian J. Chem |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-120102018-02-07T07:31:07Z http://umpir.ump.edu.my/id/eprint/12010/ Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Nurul Izwanie, Rasli TP Chemical technology Membrane chromatography has been used widely as an alternative to the conventional packed bed chromatography for protein separation. Membrane chromatography used an adsorptive membrane that carried specific chromatography functionality. In the current study, a membrane chromatography was prepared by modification of commercial polyamide microfiltration membrane with acrylic acid monomer. Two modification methods were compared which are UV photo grafting and chemical grafting via redox reaction. Modification parameters studied were initiator concentration (1-50 mM), monomer concentration (0.2-5 M) and reaction time (5-60 min). The highest lysozyme binding capacity achieved was 0.175 mg of lysozyme/cm2 membrane for the membrane prepared via UV photo grafting using 10 mM of photo-initiator, 0.1 M of acrylic acid and 15 min of reaction time. Asian J. Chem 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12010/1/Comparison%20of%20Chemical%20and%20UV%20Photo-Grafting%20Modification%20on%20Polyamide.pdf Syed Mohd Saufi, Tuan Chik and Mazrul Nizam, Abu Seman and Nurul Izwanie, Rasli (2016) Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography. Asian Journal of Chemistry , 28 (2). pp. 395-398. ISSN 0970-7077. (Published) http://dx.doi.org/10.14233/ajchem.2016.19377 DOI: 10.14233/ajchem.2013.19377 |
| spellingShingle | TP Chemical technology Syed Mohd Saufi, Tuan Chik Mazrul Nizam, Abu Seman Nurul Izwanie, Rasli Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography |
| title | Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography |
| title_full | Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography |
| title_fullStr | Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography |
| title_full_unstemmed | Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography |
| title_short | Comparison of Chemical and UV Photo-Grafting Modification on Polyamide Microfiltration Membrane for the Preparation of Membrane Chromatography |
| title_sort | comparison of chemical and uv photo-grafting modification on polyamide microfiltration membrane for the preparation of membrane chromatography |
| topic | TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/12010/ http://umpir.ump.edu.my/id/eprint/12010/ http://umpir.ump.edu.my/id/eprint/12010/ http://umpir.ump.edu.my/id/eprint/12010/1/Comparison%20of%20Chemical%20and%20UV%20Photo-Grafting%20Modification%20on%20Polyamide.pdf |