Comparison of Different Cell Disruption Methods And Cell Extractant Buffers For Recombinant Bromelain Expressed in E.COLI BL21-A1

Comparison of Different Cell Disruption Methods And Cell Extractant Buffers For Recombinant Bromelain Expressed in E.COLI BL21-A1

The often-encountered problem such as protein degradation has driven various methods of cell lysis in obtaining recombinant protein post fermentation. In this paper, we compare methods such as homogenization, sonication, sonication with lysozyme and chemical lysis using B-PER reagent with lysozyme t...

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Bibliographic Details
Main Authors: Zatul Iffah, Mohd Arshad, Azura, Amid, Muhd. Ezza Faiez, Othman
Format: Article
Language:English
English
Published: Penerbit Universiti Teknologi Malaysia 2015
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/11859/
http://umpir.ump.edu.my/id/eprint/11859/1/Comparison%20Of%20Different%20Cell%20Disruption%20Methods%20And%20Cell%20Extractant%20Buffers%20For%20Recombinant%20Bromelain%20Expressed%20in%20E.COLI%20BL21-A1.pdf
http://umpir.ump.edu.my/id/eprint/11859/7/Comparison%20of%20Different%20Cell%20Disruption%20Methods%20and%20Cell%20Extractant%20Buffers%20for%20Recombinant%20Bromelain%20Expressed%20in%20E-coli%20BL21-A1.pdf

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