Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
In this work, we have described a new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This was achieved by the electropolymerization...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2015
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| Online Access: | http://umpir.ump.edu.my/id/eprint/11548/ http://umpir.ump.edu.my/id/eprint/11548/7/akm%20kafi-fabrication%20of%203-dimensional.pdf |
| _version_ | 1848819038161993728 |
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| author | A. K. M., Kafi M. M., Yusoff S. N., Nina |
| author_facet | A. K. M., Kafi M. M., Yusoff S. N., Nina |
| author_sort | A. K. M., Kafi |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | In this work, we have described a new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This was achieved by the electropolymerization of aniline-functionalized carbon nanotubes (CNTs) and 4-aminothiophenol -modified-HRP on a 4-aminothiophenol monolayer-modified Au electrode. The synthesized 3D HRP/CNT networks were characterized with cyclic voltammetry and amperometry, resulting the establishment direct electron transfer between the redox active unit of HRP and the Au surface. Electrochemical measurements reveal that the immobilized HRP exhibits high biological activity and stability and a quasi reversible redox peak of the redox center of HRP was observed at about −0.355 and −0.275 V vs. Ag/AgCl. The electron transfer rate constant, KS and electron transfer co-efficientwere found to be 0.57 s-1 and 0.42, respectively. Based on the electrocatalytic process by direct electrochemistry of HRP, a biosensor for detecting H2O2 was developed. The developed biosensor exhibits excellent electrocatalytic activity for the reduction of H2O2. The proposed biosensor modified with HRP/CNT 3D network displays a broader linear range and a lower detection limit for H2O2 determination. The linear range is from 1.0×10−7 to 1.2×10−4M with a detection limit of 2.2.0×10−8M at 3σ. Moreover, this biosensor exhibits very high sensitivity, good reproducibility and long-time stability. In summary, ease of fabrication, a low cost, fast response and high sensitivity are the main advantages of the new biosensor proposed in this study. These obvious advantages would really help for the real analytical applicability of the proposed biosensor. |
| first_indexed | 2025-11-15T01:47:12Z |
| format | Conference or Workshop Item |
| id | ump-11548 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:47:12Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-115482018-07-26T03:30:34Z http://umpir.ump.edu.my/id/eprint/11548/ Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials A. K. M., Kafi M. M., Yusoff S. N., Nina Q Science (General) In this work, we have described a new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This was achieved by the electropolymerization of aniline-functionalized carbon nanotubes (CNTs) and 4-aminothiophenol -modified-HRP on a 4-aminothiophenol monolayer-modified Au electrode. The synthesized 3D HRP/CNT networks were characterized with cyclic voltammetry and amperometry, resulting the establishment direct electron transfer between the redox active unit of HRP and the Au surface. Electrochemical measurements reveal that the immobilized HRP exhibits high biological activity and stability and a quasi reversible redox peak of the redox center of HRP was observed at about −0.355 and −0.275 V vs. Ag/AgCl. The electron transfer rate constant, KS and electron transfer co-efficientwere found to be 0.57 s-1 and 0.42, respectively. Based on the electrocatalytic process by direct electrochemistry of HRP, a biosensor for detecting H2O2 was developed. The developed biosensor exhibits excellent electrocatalytic activity for the reduction of H2O2. The proposed biosensor modified with HRP/CNT 3D network displays a broader linear range and a lower detection limit for H2O2 determination. The linear range is from 1.0×10−7 to 1.2×10−4M with a detection limit of 2.2.0×10−8M at 3σ. Moreover, this biosensor exhibits very high sensitivity, good reproducibility and long-time stability. In summary, ease of fabrication, a low cost, fast response and high sensitivity are the main advantages of the new biosensor proposed in this study. These obvious advantages would really help for the real analytical applicability of the proposed biosensor. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/11548/7/akm%20kafi-fabrication%20of%203-dimensional.pdf A. K. M., Kafi and M. M., Yusoff and S. N., Nina (2015) Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials. In: The 3rd International Conference on Advanced Electromaterials , 17-20 November 2015 , Jeju, Korea. p. 1.. (Unpublished) (Unpublished) |
| spellingShingle | Q Science (General) A. K. M., Kafi M. M., Yusoff S. N., Nina Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials |
| title | Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
|
| title_full | Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
|
| title_fullStr | Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
|
| title_full_unstemmed | Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
|
| title_short | Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
|
| title_sort | fabrication of 3-dimensional cross-linked redox protein/nanomaterials |
| topic | Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/11548/ http://umpir.ump.edu.my/id/eprint/11548/7/akm%20kafi-fabrication%20of%203-dimensional.pdf |