Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis
Monoclonal antibodies (mAbs) are unique and specific drug molecules targeting the treatment of various diseases such as arthritis, immune disorders, infectious diseases, and cancer etc. Different methods such as antibody coupled affinity chromatography, hydrophobic interaction chromatography, etc.,...
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| Language: | English |
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Hibiscus Publisher
2015
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| Online Access: | http://umpir.ump.edu.my/id/eprint/11150/ http://umpir.ump.edu.my/id/eprint/11150/1/Optimization%20of%20Monoclonal%20Antibodies%20Purification%20Expressed%20in%20H-192%20cells%20using%20Preparative%20Native-Polyacrylamide%20Gel%20Electrophoresis.pdf |
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| author | Santhana, Krishnan Zularisam, A. W. Chua, Gek Kee Chew, Few Ne Mimi Sakinah, A. M. |
| author_facet | Santhana, Krishnan Zularisam, A. W. Chua, Gek Kee Chew, Few Ne Mimi Sakinah, A. M. |
| author_sort | Santhana, Krishnan |
| building | UMP Institutional Repository |
| collection | Online Access |
| description | Monoclonal antibodies (mAbs) are unique and specific drug molecules targeting the treatment of various diseases such as arthritis, immune disorders, infectious diseases, and cancer etc. Different methods such as antibody coupled affinity chromatography, hydrophobic interaction chromatography, etc., can be applied to purify mAbs from various sources. This article provides a simple, cost effective, preparative native-polyacrylamide gel electrophoresis (n-PAGE) technique to purify mAbs expressed in H-192 cells (Hybridoma murine cell lines) against an antigen i.e. 17-alpha-hydroxyprogesterone (17-OHP), which further can have diagnostic application to detect Congenital Adrenal Hyperplasia (CAH). Furthermore, different parameters such as concentration and volume of the feedstock (medium containing antibodies), pore size of gel, height of resolving gel etc. were optimized to obtain the maximum purity and yield of mAbs. |
| first_indexed | 2025-11-15T01:45:36Z |
| format | Article |
| id | ump-11150 |
| institution | Universiti Malaysia Pahang |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:45:36Z |
| publishDate | 2015 |
| publisher | Hibiscus Publisher |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | ump-111502018-01-17T02:29:50Z http://umpir.ump.edu.my/id/eprint/11150/ Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis Santhana, Krishnan Zularisam, A. W. Chua, Gek Kee Chew, Few Ne Mimi Sakinah, A. M. T Technology (General) Monoclonal antibodies (mAbs) are unique and specific drug molecules targeting the treatment of various diseases such as arthritis, immune disorders, infectious diseases, and cancer etc. Different methods such as antibody coupled affinity chromatography, hydrophobic interaction chromatography, etc., can be applied to purify mAbs from various sources. This article provides a simple, cost effective, preparative native-polyacrylamide gel electrophoresis (n-PAGE) technique to purify mAbs expressed in H-192 cells (Hybridoma murine cell lines) against an antigen i.e. 17-alpha-hydroxyprogesterone (17-OHP), which further can have diagnostic application to detect Congenital Adrenal Hyperplasia (CAH). Furthermore, different parameters such as concentration and volume of the feedstock (medium containing antibodies), pore size of gel, height of resolving gel etc. were optimized to obtain the maximum purity and yield of mAbs. Hibiscus Publisher 2015 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/11150/1/Optimization%20of%20Monoclonal%20Antibodies%20Purification%20Expressed%20in%20H-192%20cells%20using%20Preparative%20Native-Polyacrylamide%20Gel%20Electrophoresis.pdf Santhana, Krishnan and Zularisam, A. W. and Chua, Gek Kee and Chew, Few Ne and Mimi Sakinah, A. M. (2015) Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis. Journal Of Biochemistry, Microbiology and Biotechnology , 3 (1). pp. 21-25. ISSN 2289-5779. (Published) http://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/231 |
| spellingShingle | T Technology (General) Santhana, Krishnan Zularisam, A. W. Chua, Gek Kee Chew, Few Ne Mimi Sakinah, A. M. Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis |
| title | Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis |
| title_full | Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis |
| title_fullStr | Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis |
| title_full_unstemmed | Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis |
| title_short | Optimization of Monoclonal Antibodies Purification Expressed in H-192 cells using Preparative Native-Polyacrylamide Gel Electrophoresis |
| title_sort | optimization of monoclonal antibodies purification expressed in h-192 cells using preparative native-polyacrylamide gel electrophoresis |
| topic | T Technology (General) |
| url | http://umpir.ump.edu.my/id/eprint/11150/ http://umpir.ump.edu.my/id/eprint/11150/ http://umpir.ump.edu.my/id/eprint/11150/1/Optimization%20of%20Monoclonal%20Antibodies%20Purification%20Expressed%20in%20H-192%20cells%20using%20Preparative%20Native-Polyacrylamide%20Gel%20Electrophoresis.pdf |