Pathogenesis of mice-adapted dengue virus in adult mice infection model / Tam Kim Kee
The present study aims to study the pathogenesis of mice-adapted dengue type 2 virus (DENV-2MA) in an adult mice model towards the development of a potential animal model for dengue. This was performed by first adapting DENY- 2 in suckling mice brain, determining the virus viraemia phase and viru...
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| Format: | Thesis |
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2006
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| Online Access: | http://studentsrepo.um.edu.my/7305/ http://studentsrepo.um.edu.my/7305/1/Phathogenesis_of_Mice%2Dadapted_Dengue.pdf |
| _version_ | 1848773358912536576 |
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| author | Tam, Kim Kee |
| author_facet | Tam, Kim Kee |
| author_sort | Tam, Kim Kee |
| building | UM Research Repository |
| collection | Online Access |
| description | The present study aims to study the pathogenesis of mice-adapted dengue
type 2 virus (DENV-2MA) in an adult mice model towards the development of a
potential animal model for dengue. This was performed by first adapting DENY-
2 in suckling mice brain, determining the virus viraemia phase and virus tropism
in the infected adult mice, and evaluating specific immune response of the
infected mice against the DENY-2MA and un"d" apted dengue virus type 2 (DENY-
\
2uA)· For the adaptation of DENV-2 in suckling mice brain, DENY-2 was
inoculated into 1 day old suckling mice brain and harvested at day 7 postinfection
(p.i). This was repeated three times prior to inoculation of the
DENV-2MA into adult mice. Adult BALB/c mice (4 to 5 weeks) were infected
intraperitoneally with 1.0 x 103 foci forming unit (Ifu) D ... NV-2MA or D NV-2u
to study the virus replication in adult mice. Our finding showed that no iraemia
phase was detected in these BALB/c mice. DENV-2MA, however, was r co ered
from the infected mice brain and spleen. Study on the DENV-2MA and D NV-
2uA infected mice immune response showed that both DENY-2MA and D NV-2u
were able to elicit primary immune response and anarnne tic re pon e in mice.
The mice group that were inoculated with DENY-2MA and challenged with
DENV-2uA gave the highest IgG level in comparison to other mice group . Our
results revealed that DENV-2MA replicates better and elicits better immun
response in adult BALB/c mice when compared with D NV-2u . The D NV-
2MA, hence, makes a more uitable candidate for the tud of D NV infe tion in
II
adult mice and ought to be used for future development of an animal model for DENY infection. |
| first_indexed | 2025-11-14T13:41:09Z |
| format | Thesis |
| id | um-7305 |
| institution | University Malaya |
| institution_category | Local University |
| last_indexed | 2025-11-14T13:41:09Z |
| publishDate | 2006 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | um-73052021-01-13T23:50:13Z Pathogenesis of mice-adapted dengue virus in adult mice infection model / Tam Kim Kee Tam, Kim Kee R Medicine (General) The present study aims to study the pathogenesis of mice-adapted dengue type 2 virus (DENV-2MA) in an adult mice model towards the development of a potential animal model for dengue. This was performed by first adapting DENY- 2 in suckling mice brain, determining the virus viraemia phase and virus tropism in the infected adult mice, and evaluating specific immune response of the infected mice against the DENY-2MA and un"d" apted dengue virus type 2 (DENY- \ 2uA)· For the adaptation of DENV-2 in suckling mice brain, DENY-2 was inoculated into 1 day old suckling mice brain and harvested at day 7 postinfection (p.i). This was repeated three times prior to inoculation of the DENV-2MA into adult mice. Adult BALB/c mice (4 to 5 weeks) were infected intraperitoneally with 1.0 x 103 foci forming unit (Ifu) D ... NV-2MA or D NV-2u to study the virus replication in adult mice. Our finding showed that no iraemia phase was detected in these BALB/c mice. DENV-2MA, however, was r co ered from the infected mice brain and spleen. Study on the DENV-2MA and D NV- 2uA infected mice immune response showed that both DENY-2MA and D NV-2u were able to elicit primary immune response and anarnne tic re pon e in mice. The mice group that were inoculated with DENY-2MA and challenged with DENV-2uA gave the highest IgG level in comparison to other mice group . Our results revealed that DENV-2MA replicates better and elicits better immun response in adult BALB/c mice when compared with D NV-2u . The D NV- 2MA, hence, makes a more uitable candidate for the tud of D NV infe tion in II adult mice and ought to be used for future development of an animal model for DENY infection. 2006 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/7305/1/Phathogenesis_of_Mice%2Dadapted_Dengue.pdf Tam, Kim Kee (2006) Pathogenesis of mice-adapted dengue virus in adult mice infection model / Tam Kim Kee. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/7305/ |
| spellingShingle | R Medicine (General) Tam, Kim Kee Pathogenesis of mice-adapted dengue virus in adult mice infection model / Tam Kim Kee |
| title | Pathogenesis of mice-adapted dengue virus in adult mice infection model / Tam Kim Kee |
| title_full | Pathogenesis of mice-adapted dengue virus in adult mice infection model / Tam Kim Kee |
| title_fullStr | Pathogenesis of mice-adapted dengue virus in adult mice infection model / Tam Kim Kee |
| title_full_unstemmed | Pathogenesis of mice-adapted dengue virus in adult mice infection model / Tam Kim Kee |
| title_short | Pathogenesis of mice-adapted dengue virus in adult mice infection model / Tam Kim Kee |
| title_sort | pathogenesis of mice-adapted dengue virus in adult mice infection model / tam kim kee |
| topic | R Medicine (General) |
| url | http://studentsrepo.um.edu.my/7305/ http://studentsrepo.um.edu.my/7305/1/Phathogenesis_of_Mice%2Dadapted_Dengue.pdf |