Kinetics and improvement of biodiesel oxidative stability by a natural antioxidant from Brucea Javanica seeds / Khalilullah
The study was conducted to determine antioxidant activity of Brucea javanica seed and test its potential to be used as natural antioxidant for biodiesel. 2, 2-diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing antioxidant Power (FRAP) and Metal Chelating Assay are the methodologies used to test an...
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| Format: | Thesis |
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2015
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| Online Access: | http://studentsrepo.um.edu.my/6509/ http://studentsrepo.um.edu.my/6509/4/khalilullah.pdf |
| Summary: | The study was conducted to determine antioxidant activity of Brucea javanica seed and test
its potential to be used as natural antioxidant for biodiesel. 2, 2-diphenyl-1-picrylhydrazyl
(DPPH), Ferric Reducing antioxidant Power (FRAP) and Metal Chelating Assay are the
methodologies used to test antioxidant activity. The results indicated that higher flavonoids
and phenolic contents could be detected in ethyl acetate and methanol extracts as compared to
hexane extract. Ethyl acetate extract of B.javanica seed showed highest DPPH inhibition
activity up to 90% with (IC50= 31.2 g/ml). On the other hand, methanol extract had a highest
FRAP activity (0.180 ±0.03 mmol Fe2+ /g extract) with 71% inhibition. In the metal chelating
assay, ethyl acetate extract indicated the highest chelating activity with 59% inhibition
(IC50=299 g/ml). In addition, the bioactive compounds were also analysed using LCMS and
GCMS for confirmation. The chemical compounds found in B. javanica seed extract were
brevifolin, ellagic acid, gallic acid, quinic acid, strictinin, O-methyl ellagic acid,
protocatechuic acid and ellagic acid isomers from LCMS analyses. 35 chemical have been
identified in GCMS analyses. In order to confirm anti-oxidation effect of B. javanica seed
extract, rancimat and thermal oxidation tests were performed at 110oC and 80oC respectively.
For rancimat test, Gallic acid showed the highest oxidative stability of biodiesel up to 69
hours induction period (IP). However, on the other end, ethyl acetate crude extract at 1000
ppm suggested better stability up to 6h in thermal oxidation test. While, kinetics evaluation
showed a high degree of correlation coefficient (R2 =0.9) confirming that the degradation of
antioxidant in improving oxidative stability of biodiesel follows first order kinetics. |
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