Morphological characterization, molecular identification and pathotyping of Colletotrichum Species in Peninsular Malaysia / Khor Bee Sym
Nineteen isolates of Colletotrichum spp were obtained from infected fruits, 16 isolates were from Capsicum annuum, and three isolates from non-host of Capsicum annuum, which consist of one isolates from papayas and two isolates from strawberries. All showing anthracnose symptom. Growth rate can b...
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| Format: | Thesis |
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2013
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| Online Access: | http://studentsrepo.um.edu.my/6426/ http://studentsrepo.um.edu.my/6426/1/KHOR_BEE_SYM_SGR070079.pdf |
| Summary: | Nineteen isolates of Colletotrichum spp were obtained from infected fruits, 16
isolates were from Capsicum annuum, and three isolates from non-host of Capsicum
annuum, which consist of one isolates from papayas and two isolates from strawberries.
All showing anthracnose symptom. Growth rate can be the criteria for Colletotrichum
identification. C. gloeosporioides has the fastest growth rate among the 3 species and C.
acutatum has the slowest growth. C. truncatum has the average growth rates. ANOVA
results showed that all of growth rate showed that there are significant different among
the species. C. truncatum has the longest length of conidia (falcate) size and C.
acutatum (fusiform) has the shortest length of conidia size. C. gloeosporioides
(cylindrical) has the widest width compared to C. truncatum.
ITS has identified six isolates as C. truncatum, five isolates of C. scovillei and
five C. gloeosporioides complexes most likely as one Colletotrichum species, one C.
asianum, one C. siamense and two C. queenslandicum. More gene sequencing work
needed to clearly differentiate the species besides ITS region.
Pathotypes studies have identified on wounded mature fruits were one pathotype
on C. scovillei which were highly virulent on the genotype Capsicum annuum (Kulai)
with host reaction of 7, three pathotypes were identified within C. truncatum infecting
(Kulai and bell pepper) with host reaction of 5 to 9, four pathotypes were identified
within C. gloeosporioides complex with only two isolates infecting Kulai and bell
pepper, one isolates of Colletotrichum sp. and two isolates infecting only Kulai. All
isolates from C. annuum used in this study showed no infection on the resistant
genotypes C. chinense. Non-host Colletotrichum species were able to infect only the
genotype Kulai. |
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