Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat
Bananaandplantain(Musaspp.)arestaplefoodcropsintropicaland subtropicalcountriesandalsoplayakeyroleintheeconomicofmanydeveloping countries.However,thetenyearreview(1996-2005)oftheproductionofthecrop showedthatthelandusageforbananaandplantainhadincreasedbuttheyieldhadbeen reducedduringthesameperiod.I...
| Main Author: | |
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| Format: | Thesis |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f796$002fSD_ILS:796891/one?qu=Assessment+of+molecular+markers+associated+with+resistance http://studentsrepo.um.edu.my/4324/1/Fatimah_Kayat_%2D_PhD_Thesis_2009.pdf |
| _version_ | 1848772618202644480 |
|---|---|
| author | Kayat, Fatimah |
| author_facet | Kayat, Fatimah |
| author_sort | Kayat, Fatimah |
| building | UM Research Repository |
| collection | Online Access |
| description | Bananaandplantain(Musaspp.)arestaplefoodcropsintropicaland
subtropicalcountriesandalsoplayakeyroleintheeconomicofmanydeveloping countries.However,thetenyearreview(1996-2005)oftheproductionofthecrop showedthatthelandusageforbananaandplantainhadincreasedbuttheyieldhadbeen reducedduringthesameperiod.InMalaysia,PanamadiseaseorFusariumwiltcaused
byFusariumoxysporumf.sp.cubenseVCG01213/16alsoknownasTropicalrace4
(TR4)isthemajorandthemostdestructivediseasewhichcausesconsiderablelossto theMalaysianbananaexporttrade.Conventionalbananabreedingforresistanceto
Fusariumwiltishamperedbysterility,triploidy,longgenerationtimeandthelackof
appropriatetestingsites.Biotechnologicaladvancesinvolvinginvitropropagation,
somaticembryogenesisandmolecularmarkersprovidetoolsforgeneticrelationship
studiesandQTLanalysisthuscouldenhancethedevelopmentofcultivarsadaptedto
changingenvironments.
Theuseofgenesfromthewildcroprelativeshavebeenrecognizedinbreeding
programsofmanycrops.Inthisstudy,thestrategyapproachistodevelopnew
populationswithtraitsspecifictothepathogenfromtheindigenouswildbananaMusa
acuminatassp.malaccensis(AA)whichhasbeenshowntohaveveryhighresistanceto
FOCTR4.Maturedseedswereextractedfromfruitbunchesoffourrandomopencross
populationsofwildbananainordertoproduceseedprogeniesraisedthroughzygotic
embryoculture.Embryogerminationwasachievedwithoutanyseedtreatmentinthe
darkfortwoweeksbeforebeingplacedunderlight.Individualseedprogenieswere
subjectedtoseveralin-vitrostagesinordertogainuniformplantletswithreplicatesfor
diseasescreening.DifferentdegreesofresponsetoFusariumoxysporumf.sp.cubense
TR4wereobservedinwildbananaseedprogeniesaswellastheirF1hybridpopulations
whichprovidedapotentiallyusefulgeneticresourceforthedevelopmentofdisease
resistancemarkersthatcouldbeexploitedinamarker-assistedselection.
AhighdegreeofpolymorphisminwildbananaseedprogeniesshownbyRAPD
m
arkersprovidedgoodearlyevidenceforthepotentialusefulnessofpolymorphismandse
gregationstudiesusingmolecularmarkers.AnalysisofRAPD,STMSandAFLPm
arkersinthisstudydidnotshowanycleardifferentiationbetweenresistantandsus
ceptibleindividuals.However,markersgeneratedbythosemethodswereincludedinl
inkagemapdevelopment.Atotalof18markershadbeengeneratedfromRAPDa
nalysiswhile14SSRmarkerswereobservedfromSTMSanalysis.Atotalof4657ba
ndswasgeneratedfrom30EcoR1+3xMseI+3,oneEcoR1+3xMseI+2and43P
stI+2xMseI+3primercombinations.The639markersconsistingof607AFLPs,14S
SRsand17RAPDsmarkersweredeterminedontwomappingpopulations(resistancea
ndsusceptibilitytoFOCTR4).Atotalof471markers(286Pst-markers,155Eco-m
arkers,17RAPDmarkersand13STMSmarkers)wereanalyzedforthesusceptiblem
appingpopulationwhile414markers(249Pst-markers,143Eco-markers,9RAPDm
arkersand13STMSmarkers)wereanalyzedfortheresistantmappingpopulation.L
inkageanalysisofthedataresultedinthegenerationoftwosetsoflinkagemapsc
onsistingof32linkagegroupsfortheresistantmappingpopulationand37linkageg
roupsforthesusceptiblemappingpopulation.Themapshaveprovidedthebasisoff
uturemappingandmarker-assistedbreedingstudiesandstrategiesforthisbanana. |
| first_indexed | 2025-11-14T13:29:22Z |
| format | Thesis |
| id | um-4324 |
| institution | University Malaya |
| institution_category | Local University |
| last_indexed | 2025-11-14T13:29:22Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | um-43242014-09-27T03:39:32Z Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat Kayat, Fatimah Q Science (General) QH Natural history Bananaandplantain(Musaspp.)arestaplefoodcropsintropicaland subtropicalcountriesandalsoplayakeyroleintheeconomicofmanydeveloping countries.However,thetenyearreview(1996-2005)oftheproductionofthecrop showedthatthelandusageforbananaandplantainhadincreasedbuttheyieldhadbeen reducedduringthesameperiod.InMalaysia,PanamadiseaseorFusariumwiltcaused byFusariumoxysporumf.sp.cubenseVCG01213/16alsoknownasTropicalrace4 (TR4)isthemajorandthemostdestructivediseasewhichcausesconsiderablelossto theMalaysianbananaexporttrade.Conventionalbananabreedingforresistanceto Fusariumwiltishamperedbysterility,triploidy,longgenerationtimeandthelackof appropriatetestingsites.Biotechnologicaladvancesinvolvinginvitropropagation, somaticembryogenesisandmolecularmarkersprovidetoolsforgeneticrelationship studiesandQTLanalysisthuscouldenhancethedevelopmentofcultivarsadaptedto changingenvironments. Theuseofgenesfromthewildcroprelativeshavebeenrecognizedinbreeding programsofmanycrops.Inthisstudy,thestrategyapproachistodevelopnew populationswithtraitsspecifictothepathogenfromtheindigenouswildbananaMusa acuminatassp.malaccensis(AA)whichhasbeenshowntohaveveryhighresistanceto FOCTR4.Maturedseedswereextractedfromfruitbunchesoffourrandomopencross populationsofwildbananainordertoproduceseedprogeniesraisedthroughzygotic embryoculture.Embryogerminationwasachievedwithoutanyseedtreatmentinthe darkfortwoweeksbeforebeingplacedunderlight.Individualseedprogenieswere subjectedtoseveralin-vitrostagesinordertogainuniformplantletswithreplicatesfor diseasescreening.DifferentdegreesofresponsetoFusariumoxysporumf.sp.cubense TR4wereobservedinwildbananaseedprogeniesaswellastheirF1hybridpopulations whichprovidedapotentiallyusefulgeneticresourceforthedevelopmentofdisease resistancemarkersthatcouldbeexploitedinamarker-assistedselection. AhighdegreeofpolymorphisminwildbananaseedprogeniesshownbyRAPD m arkersprovidedgoodearlyevidenceforthepotentialusefulnessofpolymorphismandse gregationstudiesusingmolecularmarkers.AnalysisofRAPD,STMSandAFLPm arkersinthisstudydidnotshowanycleardifferentiationbetweenresistantandsus ceptibleindividuals.However,markersgeneratedbythosemethodswereincludedinl inkagemapdevelopment.Atotalof18markershadbeengeneratedfromRAPDa nalysiswhile14SSRmarkerswereobservedfromSTMSanalysis.Atotalof4657ba ndswasgeneratedfrom30EcoR1+3xMseI+3,oneEcoR1+3xMseI+2and43P stI+2xMseI+3primercombinations.The639markersconsistingof607AFLPs,14S SRsand17RAPDsmarkersweredeterminedontwomappingpopulations(resistancea ndsusceptibilitytoFOCTR4).Atotalof471markers(286Pst-markers,155Eco-m arkers,17RAPDmarkersand13STMSmarkers)wereanalyzedforthesusceptiblem appingpopulationwhile414markers(249Pst-markers,143Eco-markers,9RAPDm arkersand13STMSmarkers)wereanalyzedfortheresistantmappingpopulation.L inkageanalysisofthedataresultedinthegenerationoftwosetsoflinkagemapsc onsistingof32linkagegroupsfortheresistantmappingpopulationand37linkageg roupsforthesusceptiblemappingpopulation.Themapshaveprovidedthebasisoff uturemappingandmarker-assistedbreedingstudiesandstrategiesforthisbanana. 2009 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/4324/1/Fatimah_Kayat_%2D_PhD_Thesis_2009.pdf http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f796$002fSD_ILS:796891/one?qu=Assessment+of+molecular+markers+associated+with+resistance Kayat, Fatimah (2009) Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat. PhD thesis, University of Malaya. http://studentsrepo.um.edu.my/4324/ |
| spellingShingle | Q Science (General) QH Natural history Kayat, Fatimah Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat |
| title | Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat |
| title_full | Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat |
| title_fullStr | Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat |
| title_full_unstemmed | Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat |
| title_short | Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat |
| title_sort | assessment of molecular markers associated with resistance or susceptibility to fusarium wilt tr4 in musa acuminata ssp. malaccensis / fatimah binti kayat |
| topic | Q Science (General) QH Natural history |
| url | http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f796$002fSD_ILS:796891/one?qu=Assessment+of+molecular+markers+associated+with+resistance http://pendeta.um.edu.my/client/default/search/detailnonmodal/ent:$002f$002fSD_ILS$002f796$002fSD_ILS:796891/one?qu=Assessment+of+molecular+markers+associated+with+resistance http://studentsrepo.um.edu.my/4324/1/Fatimah_Kayat_%2D_PhD_Thesis_2009.pdf |